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Micro Identification

11/21 micro PBL exam

Group B Streptococci Gram-positive cocci, catalase-negative, facultative anaerobe, Beta-hemolytic identified by CSF or urine gram stain and CSF, urine or blood culture
Streptococcus pneumoniae Gram-positive cocci, catalase-negative, facultative anaerobe, alpha-hemolytic, does not grow in culture in presence of optochin or bile, positive Quellung test
Bacillus anthracis Gram-positive spore-forming rod, aerobe, PCR of nasal swab
Clostridium botulinum Gram-positive, spore-forming rod, anaerobe, culture in anaerobic conditions
Clostridium tetani Gram-positive, spore-forming rod, anaerobe, drumstick on gram stain with endospore at on end of rod
Listeria monocytogens Gram-positive, non-spore forming rod, facultative anaerobe, can grow at temperatures as low as 0 degrees Celsius
Neisseria meningitidis Gram-negative diplococci, facultative anaerobe, maltose and glucose fermenter, grown on chocolate agar, growth on Thayer Martin VCN
Escherichia coli Gram-Negative rod, Beta-hemolytic, Indole-positive, lactose fermenter, culture CSF can grow at 45.5 degrees Celsius, deep purple on EMB and pink-purple on MacConkey
Haemophilus Influenzae Type B Gram-negative rod, chocolate agar, ELISA studies, positive Quellung test
Yersinia Pestis Gram-negative rod, facultative anaerobe, gram stain will show bipolar staining
Francisella tularensis Gram-negative rod, obligate aerobe, requires cysteine, measure IgG antibody titer
Brucella Gram-negative rod, obligate aerobe, culture bone marrow (best), blood, liver or lymph nodes
Pasteurella multocida Gram-negative rod, facultative anaerobe
Coxiella burnetii Gram-negative obligate intracellular parasite, can grow at pH of 4.5 within phagolysosomes, has endospore form, compliment fixation test for rise in antibody
Leptospira interrogans Gram-negative spirochete, aerobic, periplasmic flagella, culture blood or CSF, ELISA of urine, PCR of serum, CSF and urine
Coccidioides immitis dimorphic fungi, systemic, spores at 25 degrees C, yeast at 37 degrees C, biopsy affected tissue, silver stain or KOH prep, Sabouraud's agar
Cryptococcus neoformans yeast only fungi, polysaccharide capsule, India Ink stain or CSF shows encapsulated yeast, antigen test of CSF
Herpes simplex virus Double-standed linear DNA virus, enveloped, Icosahedral symmetry, Tzanck prep reveals multinucleated giant cells and intranuclear inclusion bodies, direct fluorescent antibodies
Bunyaviridae Negative single-stranded RNA, 3 segments, helical symmetry, arthropod vector
Enteroviridae Positive single-stranded RNA, naked icosahedral symmetry, replication in cytoplasm
Rhabdoviridae Negative single-stranded RNA, nonsegmented, Helical nucleocapsid, replication in cytoplasm, negri bodies in CNS
Arenaviridae Negative single-stranded RNA, 2 segments, Helical symmetry, enveloped, replication in cytoplasm, examine blood for virus-specific antibody titer
Naegleria fowleri Amoeba, motile amoeba in CSF with white blood cells
Acanthamoeba Ameoba stage, cyst in brain, CSF reveals cysts and trophozoites
Toxoplasma gondii infectious oocyst, trophozoites, obligate intracellular parasite, high IgM and IgG titers, CT scan shows contrast-enhancing mass in brain
Plasmodium falciparum Blood smear reveals trophozoites, schizonts, gametocytes
Trichinella spiralis cysts in skeletal muscle, increased muscle enzymes in circulating blood, eosinophilia
Strongyloides stercoralis 2 mm long, fecal exam for larvae, eosinophilia
Taenia solium scolex: head with hooks, proglottids, cysticercus, adult can grow 2-8 meters, fecal exam for eggs and proglottids, CT scan can reveal calcified cysticerci, eosinophilia
Echinococcus granulosus hydatid cysts, CT scan shows cysts in liver or lung
Created by: schroerk