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Clonación

Ingeniería Genética

QuestionAnswer
Gene Knockout, producción de proteínas recombinantes, terapia génica Propósitos de la clonación
Electroporación, fusión con liposomas que contienen DNA, microinyección en el núcleo, uso de virus Formas de introducir DNA en células animales
Promotor, secuencia de kozac, terminador Elementos del cassette de expresión
Recombinación homóloga. Marcadores de selección : glancicovir y la inactivación de la neomicina (G418) Inserción dirigida
Transfección de ES cells e introducción en el blastocisto, Microinyección de DNA en un ovocito fertilizado, transformación genética mediada por semen, transformación genética mediante transformación de células somáticas y transferencia nuclear Métodos de producción de transgénicos
Método de totipotencia, método de huevo fertilizado Uso de células madres embrionarias
Created by: 1439805097
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