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Genetics Exam II (3)

HU: Frankel

QuestionAnswer (1)Answer (2)
translation taking nucleic acids and translating them to amino acids N/A
6 things needed for initiation 1) mRNA 2) ribosomes 3) initiator tRNA 4) initiation factors (IF-1, IF-2, IF-3) 5) magnesium ions 6)GTP N/A
"E-site" exit site where tRNA's are released
"A-site" aminoacyl binding site where next tRNA will attach
4 things needed for elongation 1) charged tRNAs 2) elongation factors(EF-Tu, EF-Ts, EF-G) 3) GTPs 4) peptidyl transferase N/A
What does GTP do in elongation? gives the energy required to transfer the charged tRNA N/A
2 things needed in termination 1) release factors (RF-1, RF-2, RF-3) 2) GTP N/A
What codons do RF-1 recognize? UAG or UAA N/A
What codons do RF-2 recognize? UGA or UAA N/A
aminoacyl-tRNA synthetase required for activation of amino acids N/A
EF-G catalyzes translocation N/A
EF-Tu & GTP places tRNA in the "A site" N/A
post-transcription occurs where? eukaryotes in the nucleus
translocation messenger moves down the ribosome (shifts one codon) N/A
peptidyl transferase catalyzing peptide bond N/A
Suedberg units sedimentation coefficients big#- big molecules small#- small molecules
30s divides into 23s, 16s, 5s (in prokaryotes) not additive
spacial DNA eukaryotic rRNA has this due to exons (not transcribed) N/A
45s divides into 28s, 18s, 5.8s (in eukaryotes) 5s comes off of different gene sequence
"P-site" peptidyl binding site where tRNA's anticodons attach to the codons of the mRNA