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molecular techniques

QuestionAnswer
Restriction enzymes are bacterial proteins that cleave DNA at specific sequences.
GAATTC is a palindrome and a restriction site
List the importance of restriction enzymes in the treatment of type I & II diabetes. The human insulin gene is removed from human DNA and is subcloned into a bacterial plasmid. The bacteria then produce human insulin which can be used to treat type I & II diabetes.
DNA is negatively charged. True
DNA is separated on gel electrophoresis based on: molecular weight.
Southern Blot is used to identify DNA.
Northern Blot is used to identify RNA.
Western Blot is used to identify protein.
PCR stands for polymerase chain reaction and is a technique used to amplify DNA.
Taq polymerase is derived from the bacteria Thermus Aquaticus commonly found in hot springs.
A gene knockout is a technique used by researchers to knockout a particular gene of interest.
A karyotype is a technique used to identify DNA chromosomal abnormalities. For example, trisomies, monosomies, and various other abnormalities can be detected by karyotypes.
Cloning is a technique used to create an exact copy of oneself.
RNAi inhibitor RNAs
RNAi is used to inhibit protein expression by inducing the degradation of mRNA.
Dicer is the enzyme responsible for cleavage of RNAi into siRNA.
RISC stands for RNA induced silencing complex and is the enzyme responsible for cleaving the endogenous mRNA.
List the diseases currently being investigated for RNAi treatment. Macular Degeneration, Hypercholesterolemia, RSV, Hepatitis C, and various others are potential diseases being investigated as potential RNAi targets.
Created by: alyssaleah
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