molecular techniques
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| Restriction enzymes | are bacterial proteins that cleave DNA at specific sequences.
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| GAATTC | is a palindrome and a restriction site
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| List the importance of restriction enzymes in the treatment of type I & II diabetes. | The human insulin gene is removed from human DNA and is subcloned into a bacterial plasmid.
The bacteria then produce human insulin which can be used to treat type I & II diabetes.
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| DNA is negatively charged. | True
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| DNA is separated on gel electrophoresis based on: | molecular weight.
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| Southern Blot | is used to identify DNA.
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| Northern Blot | is used to identify RNA.
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| Western Blot | is used to identify protein.
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| PCR | stands for polymerase chain reaction and is a technique used to amplify DNA.
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| Taq polymerase | is derived from the bacteria Thermus Aquaticus commonly found in hot springs.
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| A gene knockout | is a technique used by researchers to knockout a particular gene of interest.
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| A karyotype | is a technique used to identify DNA chromosomal abnormalities. For example, trisomies, monosomies, and various other abnormalities can be detected by karyotypes.
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| Cloning | is a technique used to create an exact copy of oneself.
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| RNAi | inhibitor RNAs
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| RNAi is used to | inhibit protein expression by inducing the degradation of mRNA.
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| Dicer | is the enzyme responsible for cleavage of RNAi into siRNA.
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| RISC | stands for RNA induced silencing complex and is the enzyme responsible for cleaving the endogenous mRNA.
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| List the diseases currently being investigated for RNAi treatment. | Macular Degeneration, Hypercholesterolemia, RSV, Hepatitis C, and various others are potential diseases being investigated as potential RNAi targets.
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Created by:
alyssaleah
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