molecular techniques Word Scramble
|
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
| Question | Answer |
| Restriction enzymes | are bacterial proteins that cleave DNA at specific sequences. |
| GAATTC | is a palindrome and a restriction site |
| List the importance of restriction enzymes in the treatment of type I & II diabetes. | The human insulin gene is removed from human DNA and is subcloned into a bacterial plasmid. The bacteria then produce human insulin which can be used to treat type I & II diabetes. |
| DNA is negatively charged. | True |
| DNA is separated on gel electrophoresis based on: | molecular weight. |
| Southern Blot | is used to identify DNA. |
| Northern Blot | is used to identify RNA. |
| Western Blot | is used to identify protein. |
| PCR | stands for polymerase chain reaction and is a technique used to amplify DNA. |
| Taq polymerase | is derived from the bacteria Thermus Aquaticus commonly found in hot springs. |
| A gene knockout | is a technique used by researchers to knockout a particular gene of interest. |
| A karyotype | is a technique used to identify DNA chromosomal abnormalities. For example, trisomies, monosomies, and various other abnormalities can be detected by karyotypes. |
| Cloning | is a technique used to create an exact copy of oneself. |
| RNAi | inhibitor RNAs |
| RNAi is used to | inhibit protein expression by inducing the degradation of mRNA. |
| Dicer | is the enzyme responsible for cleavage of RNAi into siRNA. |
| RISC | stands for RNA induced silencing complex and is the enzyme responsible for cleaving the endogenous mRNA. |
| List the diseases currently being investigated for RNAi treatment. | Macular Degeneration, Hypercholesterolemia, RSV, Hepatitis C, and various others are potential diseases being investigated as potential RNAi targets. |
Created by:
alyssaleah
Popular Biochemistry sets