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Genetics
Test 2
| Question | Answer |
|---|---|
| mRNA | encodes proteins, is translated by ribosomes |
| rRNA | multiple in lg ribosomal subunit, catalyze formation of peptide bonds |
| Ribozyme | RNA with catalytic activity |
| tRNA | contains anticodons that base pair w codons and decode into amino acids, brings amino acids to ribosomes |
| core promoter | directly recruits and binds general transcription factors and RNA polymerase |
| core promoter same or different in all genes? | same |
| basal transciption apparatus | RNA polymerase and general transcription factors |
| basal transcription apparatus required for transcription in all genes? | yes |
| transcription regulatory proteins | bind regulatory elements to repress/activate transcription |
| actions of transcriptional regulatory proteins | acetylation, deacetylation, methylation, demethylation, bromodomain, chromodomain, PHD finger, WD40 repeat |
| are transcription regulatory proteins same for all RNA polymerases? | no, specific |
| are transcription regulatory proteins same for all cell types? | no, specific |
| are transcription regulatory proteins same for all genes? | no, specific |
| enhancers | DNA regulatory elements that act at a large distance |
| reporter gene | gene with obvious phenotype that is used to study regulation of less obvious gene expresssion |
| DNA regulatory element | DNA sequence that increases/dereases gene expression |
| Types of mutations | insertion, deletion, substitution |
| Effects of mutations | frameshift, silent mutation, nonsense mutation, missense mutation |
| Stop codons | UGA, UAA, UAG |
| What is the ribosome recruited by in eukaryotes? | 5' cap (modified guanine) |
| What is the ribosome recruited by in prokaryotes? | Shine-Dalgarno sequence |
| Order of pieces of eukaryotic mRNA sequence | 5' cap, 5' UTR, start codon, ORF, stop codon, 3' UTR, poly(A) tail |
| Ways prokaryotes can't regulate gene expression | chromatin structure, mRNA processing |
| What causes a change in RNA/protein molecules? | change in regulatory DNA |
| What happens when there is a mutation at a splice site? | the intron doesn't get spliced |