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Module D IX
Technical Escort - Biological Operations
| Question | Answer |
|---|---|
| What does a single red line on the control line mean on a HHA? | Negative assay |
| What does two red lines on the control and test line mean? | Positive assay |
| What does a line on the test line and not on the control line mean? | Inconclusive assay |
| What does no lines on the assay mean? | Faulty assay |
| What should you do with all BSK and desiccant packs? | Take digital photos |
| Lack of sensitivity definition | A threshold concentration that below this concentration the assay will not be able to detect presence |
| Matrix effect definition | Often encountered when assaying environmental samples in HHAs |
| Cross-reactivity definition | When an antibody binds to the species it was designed for bit it also binds specifically to close relatives of that species |
| Hook effect definition | When too much antigen is added to the HHA which then results in a false negative |
| Serial dilution definition | Used to prevent the hook effect when analyzing suspected toxins, label 3 tubes for 1:1, 1:10 and 1:100 |
| How long do HHAs last at room temperature? and at 4-10 degrees Celsius? | 12 months and 24 months |
| Polymerase chain reaction definition | Fast and inexpensive technique used to amplify and copy small segments of DNA |
| Denaturing definition | Sample is heated so the DNA denatures or separates into two pieces of single stranded DNA |
| Annealing definition | Sample is then cooled down to allow for an enzyme called "Taq polymerase" to connect primers so that nucleotides are co connect to separated DNA |
| Extension definition | "Taq polymerase" then synthesizes-builds two new strands of DNA |
| RAPID features | Ruggedized Advanced Pathogen Identification Device, portable real-time PCR system |
| RAPID detectabilility | Anthrax, ricin, smallpox, plague, tularemia, botulism and ebola |
| RAZOR features | Stand alone, battery powered PCR instrument, requires proprietary reagent pouches, compact, lightweight, easy to use, interpret and analyzes up to 16 different agents |
| Capabilities of PCR | Produces billions of copies in short time, efficient method to identify agent, very specific and equipment automates process |
| Limitation of PCR | Agent's DNA sequence must be known, specialized equipment, reagents and training required |
Created by:
JeromeTalley
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