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Module D IX

Technical Escort - Biological Operations

What does a single red line on the control line mean on a HHA? Negative assay
What does two red lines on the control and test line mean? Positive assay
What does a line on the test line and not on the control line mean? Inconclusive assay
What does no lines on the assay mean? Faulty assay
What should you do with all BSK and desiccant packs? Take digital photos
Lack of sensitivity definition A threshold concentration that below this concentration the assay will not be able to detect presence
Matrix effect definition Often encountered when assaying environmental samples in HHAs
Cross-reactivity definition When an antibody binds to the species it was designed for bit it also binds specifically to close relatives of that species
Hook effect definition When too much antigen is added to the HHA which then results in a false negative
Serial dilution definition Used to prevent the hook effect when analyzing suspected toxins, label 3 tubes for 1:1, 1:10 and 1:100
How long do HHAs last at room temperature? and at 4-10 degrees Celsius? 12 months and 24 months
Polymerase chain reaction definition Fast and inexpensive technique used to amplify and copy small segments of DNA
Denaturing definition Sample is heated so the DNA denatures or separates into two pieces of single stranded DNA
Annealing definition Sample is then cooled down to allow for an enzyme called "Taq polymerase" to connect primers so that nucleotides are co connect to separated DNA
Extension definition "Taq polymerase" then synthesizes-builds two new strands of DNA
RAPID features Ruggedized Advanced Pathogen Identification Device, portable real-time PCR system
RAPID detectabilility Anthrax, ricin, smallpox, plague, tularemia, botulism and ebola
RAZOR features Stand alone, battery powered PCR instrument, requires proprietary reagent pouches, compact, lightweight, easy to use, interpret and analyzes up to 16 different agents
Capabilities of PCR Produces billions of copies in short time, efficient method to identify agent, very specific and equipment automates process
Limitation of PCR Agent's DNA sequence must be known, specialized equipment, reagents and training required
Created by: JeromeTalley