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Module D VI
Technical Escort - Biological Operations
| Question | Answer |
|---|---|
| Brucellosis routes of infection | Ingestion by raw milk, inhalation from pens, stables and slaughter houses, direct contact which is rare and direct inoculation from tick bites |
| Brucellosis abortus reservoir | Cattle |
| Brucellosis melitensis reservoir | Goats and sheep |
| Brucellosis suis reservoir | Swine |
| Brucellosis canis reservoir | Dogs |
| Brucellosis case fatality | 2% |
| What three steps may need to be repeated to achieve isolation during bacteria production? | Inoculation of agar plates, incubation and verification |
| What three conditions do incubators control? | Temperature, humidity and aerobic vs anaerobic |
| What two pieces of equipment confirm verification? | Hand held assays or microscopes |
| Isolation definition | Culture from unwanted organisms, requires nutrients to survive such as carbohydrates, protein/amino acid source, salts, vitamins and minerals |
| Fermentation definition | Process by which the living cell is able to obtain energy through the breakdown of glucose and other simple sugar molecules |
| Lag phase definition of bacteria growth | Copies of DNA are replicated |
| Exponential or log phase definition of bacteria growth | Bacteria multiply rapidly through binary fission |
| Stationary phase definition of bacteria growth | Bacteria growth begins to slow down |
| Death phase definition of bacteria growth | Bacteria are unable to reproduce |
| What are three ways to harvest from bacteria? | Filtration, sedimentation/decantation and centrifugation |
| What do toxins derive from? | Fungi, algae, plants and bacteria |
| What are three forms of disease for clostridium botulinum? | Food borne, wound botulism and infant botulism |
| Food borne botulism definition | Ingestion of performed toxin in foods that have not been canned or preserved properly |
| Wound botulism definition | Systemic spread of toxin produced by organisms inhabiting wounds like traumas, surgery, subcutaneous heroin injection and sinusitis from intranasal cocaine abuse |
Created by:
JeromeTalley
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