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Chemistry Specimen
Chemistry Shecimen & Controls Wooksheet
| Question | Answer |
|---|---|
| What four things does an accurate chemical analysis depend on? | Proper collection / preservation / Processing / Method of analysis (Machine) |
| What is the ideal time for lab measurements to be performed? | 1 hr after drawing blood - contents change after that |
| What is the three reasons that specimens are preserved? | 1. So that substance to be measured is not altered; 2. Samples should be retained long enough to repeat if necessary (Reflex) 3. Specimen collected in a timed sequence should be stored until they can be checked |
| What two things must you remember about controls? | Verify unopened & opened Experation dates; Control results published by lot number |
| Where do controls come from? | Large pools of serum or plasma |
| What sample is most often used for chemical analysis? | Serum (Red, Gold, Yellow Tubes) |
| Define fasting | No food or drink for 12 hours. |
| What is the difference between serum and plasma? | Plasma circulating blood contains fibrinogen clotting agents / Serum liquid portion w/ fibrinogen and clotting factures removed. |
| Which portion of blood sits on top of the clot? | Serum |
| Which part of the blood contains fibrinogen? | Plasma |
| What does FPG stand for? | Fasting Plasma Glucose |
| What is glycolysis? | Blood that as set too long and used up glucose |
| What tests require fasting? | Triglycerides (Fats), Glucose, Lipid Panel |
| Why is fasting beneficial? | Provides metabolic baseline / Eliminates lipemia (Lipids in the blood) |
| What is the actual name of the silicone barrier that separates serum & plasma? | Thyrotrophic gel |
| What does thyrotrophic gel do? | Prevent shift of substances /Prevents glycolysis |
| What vacutainer is used to collect serum and why? | Gold or red / Contains no anticoagulant |
| What is step one to processing a serum (Gold) tube? | Invert 5 x; do not centrifuge, yet |
| What is step two to processing a serum (Gold)tube? | Let sit no less then 30 mins - no more the 60 mins to form clot |
| What is step three to processing a serum (Gold)tube? | Centerfuge at least 15 mins within 1 hr of drawing |
| What is step four to processing a serum (Gold)tube? | Transfer to a labeled plastic bottle and write serum on it |
| What is step five to processing a serum (Gold)tube? | Refrigerate or freeze |
| Define hemolyzed serum | Is red instead of yellow |
| Define lipemic | Serum is milking white from fat (result of drawing too soon after eating.) |
| Define accuracy | True or actual valve (Controls check accuracy) |
| Define precision | Repeatablity - (Ablility to get the same answer twice.) |
| Define normal value | Average result of healthy individuals / (+/- 2 SD - standard deviation 95%) |
| Define Batch | Specimen (samples) analyzed at the same time |
| Define standard (also called calibrator) | Used to set machine values |
| Define control | Man made specimens used to verify machine accurate function. Must be done before pt. |
| Define proficiency survey | specimens sent to your lab to verify lab can get same readings |
| What four things does the control check for? | Reagents, machine function, operator technique, environment (Temp & humidity) |
Created by:
spawmary
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