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What is the maximum volume of blood that can be collected from a 110-lb donor, including samples for processing? 525 ml
How often can a blood donor donate whole blood? every 8 weeks
When RBCs are stored, there is a "shift to left". This means: Hemoglobin oxygen affinity increases, owing to a decrease in 2,3 DPG
The majority of platelets transfused in the United States today are: Apheresis platelets
What anticoagulant preservative provides a storage time of 35 days at 1 degree Celsius to 6 degrees Celsius for units of whole blood and prepared RBCs if an additive solution is added? CPDA-1 Citrate Phosphate Dextrose Adenine 1
What are the current storage time and temperature for platelet concentrates and apheresis platelet components? 5 days at 20-24 degrees Celsius
What is the minimum number of platelets required in a platelet concentrate prepared from whole blood by centrifugation? (90% of sampled units) 5.5x10 to the 10th power
RBCs can be frozen for 10 years
What is the minimum number of platelets required in apheresis component (90% of the sampled units) 3x10 to the power of 11
Whole blood and RBC units are stored at what temperature? 1-6 degrees Celsius
Additive solutions are approved for storage of RBCs for how many days? 42 days
One criterion used by the FDA for approval of new preservation solutions and storage containers is an average of 24-hour post-transfusion RBC survival of more than: 75%
What is the lowest allowable pH for a platelet component outdate? 6.2
Frozen and thawed RBCs processed in an open system can be stored for how many days/hours? 24 hours
What is the hemoglobin source for hemoglobin-based oxygen carriers in advanced clinical setting? Bovine and Human hemoglobin
What occurs during storage of RBCs? pH decreases
Nucleic acid amplification testing is used to test donor blood for? HCV HIV West Nile virus
It is not an FDA-approved test for quality control of platelets? Gram Stain
Prestorage pooled platelets can be stored for: 5 days
What is most common cause of bacterial contamination of platelet products? Entry of skin plugs into the collection bag
What best describes mitosis? Genetic material is duplicated, equally divided between two daughter cells
When a recessive trait is expressed, it means that: Two genes carrying the trait were present.
In a pedigree, the "index case" is another name for: Propositus
What are the four nitrogenous bases of DNA? Adenine Cytosine Guanine Thymine
What phenotype(s) could not result from the mating of a Jk(a+b+) female and a Jk(a-b+) male? Jk(a-b-)
Exon refers to: The coding region of a gene.
PCR technology can be used to: Amplify small amounts of DNA
Transcription can be defined as: Synthesis of RNA using DNA as template.
When a male possesses a phenotypic trait that he passes to all his daughters and none of his sons, the trait is said to be: X-linked dominant
When a female possesses a phenotypic trait that she passes to all of her sons and none of her daughters, the trait is said to be: X-linked recessive
DNA is replicated to: Semiconservatively from DNA
RNA is processed: after RNA is copied from protein on ribosomes
Translation of proteins from RNA takes place: On the ribosomes in the cytoplasm of the cell
Meiosis is necessary to: Generate new DNA sequences in daughter cells
Proteins and peptides are composed of: Linear arrangements of amino acids
Patient cells with Anti-A 4+ Anti-B 4+ A1 cells NEGATIVE B cells NEGATIVE What is the patient's blood type? AB
The major immunoglobulin class of anti-B in a group A individual is: IgM
What are the possible ABO phenotypes of the offspring from the mating of a group A to group B individual? O,A,B
What ABH substance(s) would be found in the saliva of group B secretor? H and A
What ABO blood group contains the least amount of H substance? A1B
An example of a technical error that can result in an ABO discrepancy is: Cell suspension is too heavy
What best describes Lewis antibodies? IgM, naturally occuring, do not cause HDFN
The Le gene codes for a specific glycosyltransferase that transfers a fucose to the N-acetylglucosamine on: Type 1 precursor chain
What substances would be found in the saliva of a group B secretor who also has Lele genes? H,B,LeB
Transformation to Lewis B phenotype after birth may be as follows: Le(a-b-) to Le(a+b-) to Le(a+b+) to Le(a-b+)
In what way do the Lewis antigens change during pregnancy? Lewis A and B decreases
A type 1 chain has: The terminal galactose in a 1-3 linkage to subterminal N-acetylglucosamine
What best describes Lewis antigens? The antigens are integral membrane proteins.
What genotype would explain RBCs typed as group A Le (a+b-)? A/A LeLe hh sese
What best describes MN antigens and antibodies? Well developed at birth, susceptible to enzymes, generally saline reactive
What autoantibody specificity is found in patients with Paroxysmal Cold Hemoglubinuria? Anti-P
What is the most common antibody seen in the bloodbank after ABO and Rh antibodies? anti-K
What blood group system is associated with resistance to P.vivax malaria? Duffy
The null K0 RBC can be artificially prepared by which treatments? DTT and glycine-acid EDTA
What antibody does not fit with the others with respect to optimum phase of reactivity? Anti-P1
What Duffy phenotype is prevalent in blacks but virtually nonexistent in whites? Fy(a-b-)
Antibody detection cells will not routinely detect which antibody specificity? anti-KpA
Antibodies to antigens in which of the following blood groups are known for showing dosage? Kidd
What antibody is most commonly associated with delayed hemolytic transfusion reactions? Anti-JkA
Anti-U will not react with which RBCs? M+N-S-s-
A patient with a Mycoplasma Pneumoniae infection will most likely to develop a cold autoantibody with specificity to which antigen? I
What antigen is destroyed by enzymes? FyA
The antibody to this high-prevalence antigen demonstrates mixed-field agglutination that appears shiny and refractile under the microscope: SdA
What is associated with causing severe immediate HTRs? Anti-Vel
What antibodies are more likely to be found in a black patient? Anti-CrA Anti-Ata Anti-Hy
A weakly reactive antibody with a titer of 128 is neutralized by plasma. What could be the specificity? Anti-Ch
An antibody reacted with untreated RBCs and DTT-treated RBCs but not with ficin-treated RBCs Anti-KpB
Antibodies that are clinically insignificant because they have not been associated with causing increased destruction of RBCs, HDFN, or HTRs.
Created by: Glyness