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Lab Exercise 2

Hemacytometer Counting blood cells on a grid under a microscope with diluted blood. Normal RBC found 4.5-5.0 X 10^6 per mm3
Polycythemia increase in number of RBCs
Anemia decreased oxygen-carrying capacity of blood
Hematocrit or Packed Cell Volume used to determine if anemia present. Centrifuging whole blood separating formed elements and platelets and plasma. Normal ranges males 41-52%, females 37-47%
Hemoglobin RBC protein for oxygen transport
Hemoglobin Concentration depends on hydration status of the patient. Used Tallquist Method. Normal range males 14-18 g/dl (grams per 100mL) females 12-16g/dl
Tallquist Method for hemoglobin concentration. place a drop of blood on special absorbent paper, once dried, compare with colors from the Tallquist card.
Mean Corpuscular Volume (MCV) average volume of an individual RBC. Normal range 80-100 fL (femtoliters = 10^-15)
Normocytic, Macrocytic, Microcytic normal sized cells, big cells, little cells
Mean Corpuscular Hemoglobin (MCH) average mass of hemoglobin in a typical RBC. Normal values 27-31 picograms per cell
Calculate MCV MCV = (Hematocrit)/[RBC} [RBC] = 5X10^6 RBC/microliter
Calculate MCH MCH = [hemoglobin(g/dl) X 10dl/L]/[RBCcount per liter]
Iron Deficiency Anemia RBCs are microcytic with low hemoglobin per cell
Vitamin B12 Deficiency RBCs are macrocytic with normal hemoglobin
ABO Blood Type A= A antigens on RBC, Anti-B antigens in plasma B = B antigens on RBC, Anti-A antigens in plasma AB = AB antigens on RBC, no anti- antigens in plasma O no antigens on RBC or in plasma
Sickle-cell Anemia genetic disease that results in the production of modified hemoglobin molecules, hemoglobin S.
Of the four types of tissue (muscle, epithelial, connective, and nervous), blood falls into which category? connective
An increase in the total # of RBCs is [blank], while a decrease is referred to as [blank]. polycythemia and anemia
What is the correlation between the total number of RBCs and the oxygen -carrying capacity of blood? As total number of RBCs increases, there is an increased oxygen-carrying capacity
After you have counted a representative sample of RBCs, what next step should you take to obtain the RBCs per mm3? multiply by the dilution factor
An abnormally high hematocrit level may be caused by either polycythemia or [blank]. dehydration
What role does hemoglobin play in metabolism? oxygen transport
Name the method used to determine hemoglobin concentration in whole blood by placing a drop of blood on a piece of paper and comparing it to standard color swatches Tallquist Method
An RBC with a larger than normal volume is referred to a macrocytic, while an RBC with a smaller than normal volume is microcytic: true or false True
Calculate mean corpuscular volume divide hematocrit (as a decimal) by the concentration of RBCs per liter
How does calculating the hemoglobin concentration differ from calculating the mean corpuscular hemoglobin? MCH refers to average mass of hemoglobin in a single RBC, where hemoglobin concentration refers to mass of hemoglobin in whole blood
A patient presents to the ED with lightheadedness after a recent injury involving blood loss. The pt has a known diagnosis of chronic anemia. What is one lab test you could order to assess her current condition? Total red blood cell count, hematocrit, or hemoglobin concentration
What abnormalities would you expect to see in MVC/MCH in a patient with iron deficiency anemia? microcytic RBCs and low hemoglobin concentrations per cell
What abnormalities would you expect to see in MVC/MCH in a patient with Vitamin B12 deficiency? Macrocytic RBCs with normal hemoglobin concentrations per cell
An individual with Type B blood would have [blank] antigens on the surface of the RBCs and make anti-[blank] antibodies B and A
An individual with Type AB blood would have [blank] antigens on the surface of the RBCs and make anti-[blank] antibodies A+B and none
All those individuals with Rh- blood carry anti-Rh antibodies in the blood: true or false false
What type of hemoglobin is found in the RBCs of sickle cell anemia patients, which cause their cells to take on a crescent shape when deoxygenated? Hemoglobin S
What is the name for the counting chamber used in class during the total RBC count activity? hemocytometer
After centrifuging a sample of whole blood, what material appears at the top of the capillary tube? plasma
An abnormally low hematocrit level always means the patient is anemic: true or false false, could be overhydration
Which blood types in a woman would be concerning if she was carrying her second pregnancy? a negative blood type
The term [blank] refers to an overall increase in the total number of white blood cells, while [blank] refers to an overall decrease leukocytosis and leukopenia
Which leukocyte contains a bilobed nucleus and red-orange cytoplasmic granules? Eosinphils
Created by: connorquinby