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Microbiology Study of microscopic organisms
Bacteria / two named Systems First /Genus first (always capitalized ) Second / name of the species (not capitalized
Characteristics of bacteria Posses individual characteristics Used for criteria for recognition includes Structure / morphology / staining
Basic bacteria cell structure Every cell contains DNA Which carries the genetic information It's a single celled organizum that pocesses A cell membrane Cell wall Cell nucleus
Cell wall Known as a capsule Which can make them resistant to certain antibiotics And protect them from attack by WBC
Spores Extremely hard to kill Are resistant to heat /freezing/radiation and certain chemicals
Monophology of bacteria Most commonly caused caused disease in humans are bacteria and viruse
Bacteria is caterorized according to There morphology / shape / and reaction to gram stain
Microscopic organism Bacteria / viruses/ parasites/ fungi
Culture A group of microbes growing on nutrient-rich media
Agar Gelatin - like substance that may contain additives and nutrients that will support the growth and multiplication of microorganism
Aerobic Class of bacteria that requires oxygen to grow
Inoculation Applying a microorganism onto a culture media
Opportunistic infections Infection that developes in compromised patients and those taking certain medications such as immunosuppressive drugs
Pathogenic Disease producing
Pathogen Disease causing microorganism
Pure culture Culture that contains only one organism
Sensitivity testing Technique that evaluates which antibiotic will destroy a particular pathogen
Taxonomy Classification of living organisms into the proper category using a specific set of laws and principles
Bacilli Rod - shaped bacteria May be spore forming Can have a straight, rounded, or pointed end Can be motile ( moveable ) with flagella
Cocci Round - shaped bacteria Can occur in pairs ( diplococci ) Chains ( streptococci ) Clusters ( staphylococci )
Culture medium Liquid or solid material in which bacteria are grown
Normal flora Microorganism Normally present in different parts of the human body which pose no health threats under ordinary circumstances
Incubation Place a culture in an apparatus that provides optimum conditions for growth and multiplication of microorganisms
Fungi Group of microorganisms that includes yeast and mold
Parasite Organism that lives within upon or at the expense of the host
Gram positive Term used to describe bacteria that stain purple during the gram staining process
Anaerobic Class of bacteria that do not require oxygen to grow
Bacteria Single-celled microbes that lack a nucleus ( the most prevalent of all microorganism )
Colonies Visible growth of microorganism that appear on a culture medium
Reportable organisms Salmonella / Shigella / Sexually transmitted disease
Basic bacterial cell structure Cell membrane / cell wall/ nucleus
Cell division Takes in nutrients through cell division
Spirilla Spiral shaped Like a cork screw Most are motile. ( can move)
Mono Single ( 1. Fragment)
Diplo Two. Bacteria occurring in pairs
Strepto Bacteria occurring in chains
Staphylo Bacteria. Occurring in clusters
Stains Basic or acidic
Stains / simple Illustrates the structure of the cells
Stains / differential Produces variable results based on the composition of the cell wall
Gram stain Differential. By there color reaction to various strains Stained with primary purple stain Flushed with a mordant or fixative Treated with decolorizer
Gram stain Gram positive - bacteria remains purple Gram negative - appear pink in color
Aerobic Bacteria that requires oxygen for growth
Anaerobic Bacteria that grows only in the absence of oxygen
Growth media Used to grow microorganism Can be liquid broth Semisolid Or agar
Enriched growth media Contains additives to encourage growth of some bacteria But inhibits others
Selective growth media Contains additives to promote growth of one bacteria only and inhibits other
Differential or indicator media Contains substances that will alter the appearance of the colonies
Streptococcus pyogenes Strep throat
Kirby - Bauer To find antibiotic Sensitivity testing (R-resistant) / don't use (I - intermediate) kill some (S- sensitivity/ susceptible ) the best one
Microscopic techniques Fixed state - a spear is prepared from the specimen or culture Living state - organism is suspended in liquid and examined, prepared by wet mount or hanging drop proceedure
Wet mount Place a drop of bacterial suspension on a slide Cover drop with cover slip cover slip edges are covered with petroleum jelly to form a seal
Hanging drop Place specimen on a cover slip rim with petroleum jelly Place a clean glass slide with a well over the cover slip Invert the slide so the drop hangs from the cover slip
Virolgy Viruses are the most common cause of infectious diseases in humans Aka flu , measles, mumps, common cold
Viruses Can only multiple after entering another living cell Viruses are the smallest microorganisms ( viewed under an electronic microscope)
Methods used to identify viruses Cell culture Direct detection zero diagnosis
Parasitology Parasites are organisms that live in , on , or at the expense of the host
Vaginosis A wet mount is valuable when trying to diagnose the cause of Vaginosis
Bacitracin Test Used for identifying group A beta strep
Beta hemolysis Is a wide clear zone around the colonies on a blood agar plate
Salmonella Get from food
Shigella Air Bourne
STD's Sex
Staphylococcus pyogenes Necrotizing fasciitis / sore throat
Straphyococcus aureus Organism is known to cause wound infection
Created by: Tbella



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