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Carnegie Chem

Chemistry review sheet Specimen Collection + Quality Control

What 4 things does an accurate chemical analysis depend on? proper collection, preservation, processing, method of analysis
What is the ideal time for lab measurements to be performed? one hour
Why are specimens preserved? retained long enough to repeat and for reflex testing
What is the difference between plasma and serum serum, liquid portion with fibrinogen and clotting factors removed
What sample is most often used for checmical analysis? serum
What are the advantages of a fasting blood sample? reflects metabolic baseline
What methods are used to preserve specimens?
What is thixotropix gel used for? to separate the serum from the clot or the plasma from the cells, prevents shift of substances from the cells into the liquid
Which vacutainer is used to collect serum and why? gold and red gray and red; no anticoagulant in tube
How is a gold top tube processed? invert 5 times, allow to clot upright at least 30, no more than 60 minutes at RT, clot activator = 30 min, red glass = 60 minutes; balance centrifuge; centrifuge 10-15 minutes
Define Hemolyzed serum serum or plasma clear red
Define Icteric/ jaundiced, hyperbilirubinemia serum or plasma brownish yellow, pt might have gallstones or hepatitis
Define Lipemic serum or plasma milky white
Define Accuracy the true or actual total value
Define Precision the ability to get the same answer again (repeatability)
Define Normal Value average result of healthy individuals 95%
Define Batch or run specimens analyzed at the same time
Define Calibrator used to set machine values
Define Control must fall within an acceptable range before patient values are reported
What four things does the control check for? reagents, instrument, operator technique, environment, temp + humidity
What are important facts about controls? there are unopened + opened expiration dates: discard 1st date; results are published by lot #; QC for waived tests per kit manufacturer: per lot #, per shipment; keep records for 2 years
What steps should you follow if a control falls outside of the acceptable range?
Define Duplicate determination testing one of the samples twice; checks precision
Define proficiency survey
What is a fasting blood sample No food or beverages other than water and meds for at least 12 hours
Created by: ma2b