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Carnegie Chem
Chemistry review sheet Specimen Collection + Quality Control
| Question | Answer |
|---|---|
| What 4 things does an accurate chemical analysis depend on? | proper collection, preservation, processing, method of analysis |
| What is the ideal time for lab measurements to be performed? | one hour |
| Why are specimens preserved? | retained long enough to repeat and for reflex testing |
| What is the difference between plasma and serum | serum, liquid portion with fibrinogen and clotting factors removed |
| What sample is most often used for checmical analysis? | serum |
| What are the advantages of a fasting blood sample? | reflects metabolic baseline |
| What methods are used to preserve specimens? | |
| What is thixotropix gel used for? | to separate the serum from the clot or the plasma from the cells, prevents shift of substances from the cells into the liquid |
| Which vacutainer is used to collect serum and why? | gold and red gray and red; no anticoagulant in tube |
| How is a gold top tube processed? | invert 5 times, allow to clot upright at least 30, no more than 60 minutes at RT, clot activator = 30 min, red glass = 60 minutes; balance centrifuge; centrifuge 10-15 minutes |
| Define Hemolyzed serum | serum or plasma clear red |
| Define Icteric/ jaundiced, hyperbilirubinemia | serum or plasma brownish yellow, pt might have gallstones or hepatitis |
| Define Lipemic | serum or plasma milky white |
| Define Accuracy | the true or actual total value |
| Define Precision | the ability to get the same answer again (repeatability) |
| Define Normal Value | average result of healthy individuals 95% |
| Define Batch or run | specimens analyzed at the same time |
| Define Calibrator | used to set machine values |
| Define Control | must fall within an acceptable range before patient values are reported |
| What four things does the control check for? | reagents, instrument, operator technique, environment, temp + humidity |
| What are important facts about controls? | there are unopened + opened expiration dates: discard 1st date; results are published by lot #; QC for waived tests per kit manufacturer: per lot #, per shipment; keep records for 2 years |
| What steps should you follow if a control falls outside of the acceptable range? | |
| Define Duplicate determination | testing one of the samples twice; checks precision |
| Define proficiency survey | |
| What is a fasting blood sample | No food or beverages other than water and meds for at least 12 hours |
Created by:
ma2b
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