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Transplantation Imm.
Transplantation Immunology
Question | Answer |
---|---|
Rejection occurs due to ? | * immune recognition and response to foreign histocompatibility antigens |
most important antigens of rejection ? | MHC..... * but there are others, so can still reject if all MHCs all line up perfectly |
What is the normal role of HLA antigens in the immune response? | * Present antigenic peptides to T cells |
HLA class 1 = ? | * HLA - A, B, C.... * also expresses B2-microglobulin.... * All are in MHC-I |
HLA class 2 = ? | * All in MHC-II.... * are HLA - DR (a/b), DP (a/b), and DQ (a/b) |
How many different HLA molecules are on the cell? | * you have both on each cell |
expression of alleles of HLA ? | * get codominance with Haplotype inheritance of MHC antigens.... * each person has 2 different halotypes for each HLA bc of two parents, but are codominantly expressed |
Hyperacute Rejection ? | * happens in minutes-hrs and is from anti-donor ABs and complement |
Acute Rejection ? | * days-weeks and is from cell mediated and humoral .... * primary activation of immune system and T-Cells recognize foreign HLA antigens.... * if Cd4+ is seen, then complement and Ab also mediates this |
Chronic Rejection ? | * time frame is months-years and MOA is unclear |
One of the top drugs for anti-rejection ? | * cyclosporine - but have to ween off c it can cause kidney issues |
Testing for Donor-Recipient Compatibility in Transplantation ? | * 1. ABO Blood typing -- 2. HLA identification (tissue typing) -- 3. Antibody Screening (reactive with HLA) -- 4. Genotyping with PCR and other molecular techniques -- 5. Crossmatching – see if recipient has preformed Abs against donor |
Why have we been using more pig transplants ? | * lack of human cadaver parts |
Hematopoietic Stem Cell Transplantation (HSCT) use and marker for them in the blood ? | * Used to treat malignancy, immunodeficiency, bone marrow defects, autoimmunity ..... * CD34 marker |
Graft-versus-host disease ? | * complication of allogeneic HSCT is graft-versus-host disease (GvHD) ..... * GvHD is caused by mature donor T cells contaminating the HSC inoculum, which can recognize HLA |
How to help GVHD ? | * help eliminate as many as those mature T-cells as possible, bc once activated, it is pretty much impossible to stop |
Acute GVHD = ? | * about 3 weeks |
Chronic GVHD = ? | * months |
Helps GVHD a lot ? | * matching of the HLA antigens |