UND 363 alc. blu 2.5 Word Scramble
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| Question | Answer |
| What gives the blue color of alcian blue | copper |
| Is alcian Blue an acidic or basic dye | basic dye |
| At 2.5 ph what are stained with alcian blue | carboxylated and sulfated acid mucosubstances |
| At PH 1.0 what are stained with alcian blue | ONLY sulfated acid mucosubstances are stained |
| What is used in conjunction with alcian blue to differientated epithelial and connective mucin | hyaluronic acid (which preceeds the alcian blue staining in the process) |
| what mucin will alcian blue not stain | NEUTRAL mucins |
| What is the purpose of alcian blue 2.5 | Demonstrates acid mucopolysaccharides (ie connective tissue mucins *PAS Negative*) |
| what is the principle behind alcian blue 2.5 | forms salt link w/ the acid group of acid mucopolysaccharides |
| what are the preferred fixatives for A.B. 2.5 | 10% NBF or Bouins |
| what is the preferred thickness and control specs | 4-5microns, use colon, sm. intestine, appendix |
| what is the proper microscope for A.B. 2.5 | light |
| what are the three major reagents for A.B. 2.5 | acetic acid 3%, alcian blue, nuclear fast red |
| Acetic acid 3% is used twice in A.B. 2.5 why? | pre alcian blue to protect PH, post alcian blue to prevent non-specific staining |
| what will alcian blue and nuclear fast red do in the A.B. 2.5 tech. | Alcian blue stains the mucin, nuclear fast red will counterstain |
| what are the expected results with A.B 2.5 | weakly acidic sulfated mucosubstances, hyaluronic acid and sialomucins will stain dk. blue, Background (nucleus and cytoplasm) will be pink to red |
| what will incomplete hydration (during deparrafin/hydration steps cause) | hydration is important because some alcianophilic structures hydrate slowyly, if not completely hydrated they will stain weakly. |
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mustangvxd
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