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UND 363 alc. blu 2.5

UND 363 Alcian Blue Ph 2.5

QuestionAnswer
What gives the blue color of alcian blue copper
Is alcian Blue an acidic or basic dye basic dye
At 2.5 ph what are stained with alcian blue carboxylated and sulfated acid mucosubstances
At PH 1.0 what are stained with alcian blue ONLY sulfated acid mucosubstances are stained
What is used in conjunction with alcian blue to differientated epithelial and connective mucin hyaluronic acid (which preceeds the alcian blue staining in the process)
what mucin will alcian blue not stain NEUTRAL mucins
What is the purpose of alcian blue 2.5 Demonstrates acid mucopolysaccharides (ie connective tissue mucins *PAS Negative*)
what is the principle behind alcian blue 2.5 forms salt link w/ the acid group of acid mucopolysaccharides
what are the preferred fixatives for A.B. 2.5 10% NBF or Bouins
what is the preferred thickness and control specs 4-5microns, use colon, sm. intestine, appendix
what is the proper microscope for A.B. 2.5 light
what are the three major reagents for A.B. 2.5 acetic acid 3%, alcian blue, nuclear fast red
Acetic acid 3% is used twice in A.B. 2.5 why? pre alcian blue to protect PH, post alcian blue to prevent non-specific staining
what will alcian blue and nuclear fast red do in the A.B. 2.5 tech. Alcian blue stains the mucin, nuclear fast red will counterstain
what are the expected results with A.B 2.5 weakly acidic sulfated mucosubstances, hyaluronic acid and sialomucins will stain dk. blue, Background (nucleus and cytoplasm) will be pink to red
what will incomplete hydration (during deparrafin/hydration steps cause) hydration is important because some alcianophilic structures hydrate slowyly, if not completely hydrated they will stain weakly.
Created by: mustangvxd
 

 



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