Laboratory Science Test and Answers for MLT ASCP BOC MICRO

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1.

35) When performing a Kovac indole test, the substrate must contain: a. indole b. tryptophan c. ornithine d. paradimethylaninobenzaldehyde

2.

5) The steam autoclave method of sterilization: a. uses 15 lbs. of pressure for 15 minutes b. utilizes dry heat for 20 minutes c. produces a minimum temp of 100 C d. requires a source of ethylene oxide

3.

15) The procedure that assures the most accurate detection of mecA-mediated oxacillin resistance in routine broth microdilution susceptibility testing against S aureus is: a. addition of 4% NaCl b. incubation at 37 d. use of cefoxitin for testing

4.

155) The stock cultures needed for quality control testing of motility are: a. Salmonella typhimurium / Escherichia coli b. Escherichia coli / Pseudomonas aeruginosa d. Klebsiella pneumoniae / Escherichia coli

5.

156) The stock cultures needed for quality control testing of oxidase production are: a. Escherichia coli / Klebsiella pneumoniae b. Salmonella typhimurium / Escherichia coli c. Escherichia coli / Pseudomonas aeruginosa d. Proteus mirabilis / Escheric

6.

341) Differentiation of Mycobacterium avium from Mycobacterium intracellulare can be accomplished by: a. nitrate reduction test b. Tween hydrolysis test c. resistance to 10 ug thiophene-2-carboxylic acid hydrazide (TCH) d. DNA probe

7.

89) Which of the following combinations of organisms would be appropriate as controls to test the functions listed?

8.

60) The optimal wound specimen for cultures of anaerobic organisms should be:

9.

61) A 21 yr. old patient presents with pharyngitis. A throat swab is collected and submitted for anaerobic culture. This specimen should be:

10.

58) When processing throat swabs for a group A streptococcus culture, the medium of choice is: a. sheep blood agar b. rabbit blood agar c. human blood agar d. horse blood agar

11.

115) Streptococcus pneumoniae can be differentiated best from the viridans group of streptococci by: a. Gram stain b. the type of hemolysis c. colonial morphology d. bile solubility

12.

51) Vibrio parahaemolyticus can be isolated best from feces on: a. eosin methylene blue agar b. Hektoen Shigella agar c. Salmonella Shigella agar d. thiosulfate citrate bile salts agar

13.

26) Which of the following specimen requests is acceptable? a. feces submitted for anaerobic culture b. Foley catheter tip submitted for aerobic culture c. rectal swab submitted for direct smear for gonococci d. urine for culture of acid-fast bacilli

14.

163) A small, gram-negative bacillus is isolated from an eye culture. It grows only on chocolate agar and is oxidase-variable. The most likely organism is: a. Acinetobacter lwoffii b. Haemophilus influenzae c. Stenotrophomonas maltophilia

15.

91) The Gram stain from a blood culture shows gram-positive cocci in chains. No growth occurs on blood agar plates incubated both aerobically and anaerobically. Additional testing should be done to detect the presence of:

16.

117) A beta-hemolytic, catalase-positive, gram-positive coccus is coagulase negative by the slide coagulase test. Which of the following is the most appropriate action in identification of this organism?

17.

1) Proper media for culture of a urethral discharge from a man include: a. sheep blood and phenylethyl alcohol agars b. eosin methylene blue and sheep blood agars d. chocolate agar and modified Thayer Martin agar

18.

9) A penicillin resistant Neisseria gonorrhoeae produces: a. alpha-hemolysin b. beta-lactamase c. enterotoxin d. coagulase

19.

11) The most sensitive substrate for the detection of beta-lactamases is: a. penicillin b. ampicillin c. cefoxitin d. nitrocefin

20.

139) Which of the following organisms can grow in the small bowel and cause diarrhea in children, travelers' diarrhea, or a severe cholera-like syndrome through the production of enterotoxins? a. Yersinia enterocolitica b. Escherichia coli c. Salmonell

A.

a. sheep blood agar a (Sheep blood agar is preferred because clear-cut patterns of hemolysis are obtained.

B.

d. bile solubility d (Bile solubility testing of alpha-hemolytic streptococci differentiates S pneumoniae [soluble] from other alpha-hemolytic streptococci such as viridans streptococci [insoluble].)

C.

b (Growth only on chocolate agar is typical for Haemophilus influenzae, which is a gram-negative coccobacillus that causes upper respiratory infections.)

D.

b. tryptophan b (The indole test is used for determining an organism's ability to produce indole from deamination of tryptophan by tryptophanase.)

E.

d. urine for culture of acid-fast bacilli d (Urine is an appropriate specimen for the detection of renal tuberculosis. Since feces contain anaerobic organisms as part of the indigenous flora, it is an unacceptable specimen for anaerobic culture.

F.

d. thiosulfate citrate bile salts agar d (TCBS is a highly selective and differential medium for the recovery of most Vibrio species including V parahaemolyticus.

G.

b. Escherichia coli b (E coli can produce several different types of toxins that result in different gastroenteritis manifestations.)

H.

d. Klebsiella pneumoniae / Escherichia coli d (Of the organisms listed only Klebsiella pneumoniae is nonmotile.)

I.

d. chocolate agar and modified Thayer Martin agar

J.

c. Escherichia coli / Pseudomonas aeruginosa c (Of the organisms listed only Pseudomonas aeruginosa is oxidase positive.)

K.

d. use of cefoxitin for testing d (Cefoxitin is used as a surrogate for mecA-mediated oxacillin resistance in S aureus.

L.

d. nitrocefin (The chromogenic cephalosporin test using nitrocefin is the most sensitive and specific test for detection of beta lactamase

M.

d. do a tube coagulase test to confirm the slide test d (Staphylococcus aureus are usually beta-hemolytic. Some strains may not produce bound coagulase detected by the slide coagulase test. A tube coagulase is performed to detect free coagulase and sho

N.

. DNA probe (Mycobacterium avium and M intracellulare have the same biochemical pattern, but can be differentiated by the use of DNA probes.)

O.

d. indole - negative Escherichia coli and Proteus mirabilis d (Of the combinations listed, the use of E coli and Proteus mirabilis will produce a positive and negative result for indole, respectively

P.

b. beta-lactamase he production of beta-lactamase [penicillinase] breaks open the beta lactam ring of penicillin, destroying its activity. Thus, N. gonorrhoeae that produce beta lactamase are resistant to penicillin.)

Q.

b. rejected as unacceptable Anaerobic bacteria do not cause pharyngitis. The most common cause of pharyngitis is Streptococcus pyogenes.

R.

c. Abiotrophia defectiva c (Nutritionally deficient streptococci such as Abiotrophia do not grow on sheep blood agar without the addition of cysteine or proximity to S aureus colonies.)

S.

a. uses 15 lbs. of pressure for 15 minutes a (The traditional gravity displacement steam sterilization cycle is 121 C for 15 minutes at 15 pounds per square inch. Ethylene oxide is an alternative sterilization method.

T.

c. a syringe filled with pus, obtained before administration of antibiotics

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MLT ASCP BOC MICRO Test