SPECIAL STAINS
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| show | Kinyoun acid-fast stain
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| show | Kinyoun acid-fast stain
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| show | Carbol-fuchsin
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| show | Carbol-fuchsin method
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| Although 10% NBF is preferred, others m/b used, avoid carnoy | show 🗑
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| 4 to 5 microns | show 🗑
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| show | Kinyoun acid-fast stain
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| use millipore water in water bath | show 🗑
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| negative control from same day work load must be run | show 🗑
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| show | Kinyoun acid-fast stain
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| overcountestaining w/methelyne blue will amsk any organisms present | show 🗑
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| if section is overstained take it back to the acid-alcohol to remove methylene blue, wash with water and repeat counterstaining step | show 🗑
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| If section is allowed to dry after the carbol fuchsin, a compound that resist decolorization will be formed. repeat attempts to remove compound results in complete decolorization | show 🗑
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| show | Kinyoun acid-fast stain
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| demonstrate acid mucopolysaccharides | show 🗑
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| alcian blue is a copper phthalocyanin basic dye that is water soluble and colored blue bcuz ofits copper content | show 🗑
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| show | Alcian blue
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| when used in 3% acetic acid soluton (pH 2.5), alcian blue stains both sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycogen) | show 🗑
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| show | Alcian blue pH 2.5
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| show | Alcian blue pH 2.5
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| show | Alcian blue pH 2.5
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| show | Kinyoun acid-fast stain
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| Background - Light blue | show 🗑
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| show | Alcian blue pH 2.5
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| background - pink to red | show 🗑
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| show | Alcian blue pH 1.0
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| show | Alcian blue pH 1.0
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| acid mucopolysaccharrides and sialomucins that are carboxylated only will not be stained | show 🗑
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| Fixation 10% NBF or Bouin | show 🗑
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| show | Alcian blue pH 1.0
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| show | Alcian blue pH 1.0
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| show | Alcian blue pH 1.0
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| Background - pink to red | show 🗑
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| differentiate epithelia and connective tissue mucins | show 🗑
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| show | Alcian blue w/hyaluronidase
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| Fixation 10% NBF | show 🗑
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| 4 to 5 microns | show 🗑
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| show | Alcian blue w/hyaluronidase
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| control - umbilical cord (with and without). small bowel, appendix or colon for second control to demonstrate epithelial mucins | show 🗑
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| w/o digestion, acid mucopolysaccharides and sialomucins - deep blue | show 🗑
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| show | Alcian blue w/hyaluronidase
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| differentiate between neutral and acidic mucosubstances | show 🗑
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| show | Alcian blue PAS hematoxylin
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| show | Alcian blue PAS hematoxylin
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| 4 to 5 microns | show 🗑
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| show | Alcian blue PAS hematoxylin
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| show | Alcian blue PAS hematoxylin
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| Certain substances will be colored by both PAS and alcian blue - purple | show 🗑
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| Fixation 10% NBF, carnoy or alcoholic formalin, (AVOID CHROMATE FIXATIVES) | show 🗑
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| 4 to 5 microns | show 🗑
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| show | Muller-mowry colloidal iron
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| if strong background staining is noted, fresh solutions should be prepared and stain should be repeated. | show 🗑
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| show | Alkaline congo red
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| Green birefringence following congo red stain, is considered most specific technique for demonstration of amyloid | show 🗑
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| show | Alkaline congo red
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| derives from benzidine, can react w/cellulose | show 🗑
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| show | Amyloid
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| show | Alkaline congo red
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| show | Congo red
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| show | Alkaline congo red
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| show | Alkaline congo red
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| cut 8 to 10 microns | show 🗑
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| if sections that are not between 8 to 10 microns, may not show green birefringence | show 🗑
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| control - section w/amyloid | show 🗑
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| don not keep too may control sections cut, as the staining intensity has been reported to decrease with age of the section | show 🗑
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| massive presumably long-standing deposits give less intense histochemical reaction than small, newly formed deposits | show 🗑
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| Amyloid - deep pink to red | show 🗑
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| elastic tissue - pale pink | show 🗑
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| show | Alkaline congo red
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| following congo red, bright apple-green birefringence exhibited under polarizing light is specific for amyloid | show 🗑
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| false-positive birefringence is caused by excess dye retained in the tissue | show 🗑
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| sodium chloride and high alcohol content present in dye, tend to depress dye ionization and acid-base type staining, results in a stained sectin w/a clean background | show 🗑
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| saturation of solutions is very important, follow instructions carefully | show 🗑
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| is an intrinsic property of the amyloid fibril-congo red complex and is a function of the parallel alignment of dye molecules and amyloid fibrils | show 🗑
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| section too thin, show red faint color | show 🗑
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| show | Alkaline congo red
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| rapid screening for amyloid | show 🗑
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| show | Crystal violet
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| amyloid will induce only weak metachromasia w/thionine and toluidine blue | show 🗑
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| mixtures of basic dye | show 🗑
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| show | Crystal violet
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| show | Crystal violet
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| Fixative - 10% NBF or Alcohol | show 🗑
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| show | Crystal violet
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| show | Crystal violet
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| show | Crystal violet
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| show | Crystal violet
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| Bleeding) diffusion into surrounding mounting medium, of basic aniline dyes tends to occur w/aqueous mounting media | show 🗑
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| show | Crystal violet
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| show | Crystal violet
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| good method for amyloid not as specific as congo red w/polirization | show 🗑
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| show | Thioflavine T
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| show | Thioflavine T
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| show | Thioflavine T
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| Control - section containing amyloid | show 🗑
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| Amyloid- Fluoresces yellow to yellow-green | show 🗑
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| show | Thioflavine T
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| show | Thioflavine T
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| lipid granules, jusxtaglomerular granules and mast cells may give a yellow fluorescence but should be differentiated easily from amyliod | show 🗑
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| show | Mayer mucicarmine
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| show | Mayer mucicarmine
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| aluminum forms a chelation complex with the carmine, resulting compound has a net positive charge and attaches to the acid group of the mucin | show 🗑
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| show | Mayer mucicarmine
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| show | Mayer mucicarmine
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| show | Mayer mucicarmine
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| Mucin - deep rose/red | show 🗑
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| Capsule of cryptococcus - deep rose to red | show 🗑
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| show | Mayer mucicarmine
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| show | Mayer mucicarmine
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| show | Mayer mucicarmine
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| show | Mayer mucicarmine
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| term used to describe intracellular secrections of a variety of cells | show 🗑
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| stain with basic dye, metachromatic, precipitated by acetic acid (except gastric mucin) soluble in alkaline solutions | show 🗑
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| appear to be microscopically similar, they differ in composition | show 🗑
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| demonstrate polysaccharides, neutral mucosubstances and basement membranes | show 🗑
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| oxidation of certain tissue elements to aldehydes by periodic acid. | show 🗑
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| show | PAS reaction
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| treat basic fuchsin w/sulfurous acid | show 🗑
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| show | PAS reaction
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| show | leucofuchsin
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| show | PAS reaction
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| show | PAS reaction
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| show | PAS reaction
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| 4 to 5 microns | show 🗑
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| show | PAS reaction
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| if demonstrating glycogen use liver containing glycogen or a section of cervix(include both endo and ecto cervix) | show 🗑
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| show | All show a positive PAS raction (Bright rose)
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| schiff staining after periodate does not demonstrate presence of carbohydrate residues | show 🗑
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| show | PAS reaction
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| show | intensitity of staining in routine PAS reaction is due to the folling factors
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| show | PAS reaction
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| show | PAS reaction
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| highly chlorinated water is capable of oxidation. if section is transferred directly into tap water, any adsorbed schiff reagent m/b reoxidized to basic fuchsin,which may stain the section | show 🗑
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| show | PAS reaction
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| show | PAS reaction
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| a control slide should be run through all steps of the procedure excep the periodate oxidation step | show 🗑
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| show | PAS reaction
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| show | Bodian
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| demonstrate polysaccharides, neutral mucosubstances and basement membranes | show 🗑
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| show | PAS reaction
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| reactive group is the 1,2 glycol group, other groups are also oxidized | show 🗑
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| show | preparing schiffs reagent
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| reduction casues loss of quinoid structures and masking of the chromophores | show 🗑
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| colorless compound | show 🗑
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| show | PAS reaction
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| show | PAS reaction
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| show | PAS reaction
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| 4 to 5 microns | show 🗑
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| show | PAS reaction
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| if demonstrating glycogen use liver containing glycogen or a section of cervix(include both endo and ecto cervix) | show 🗑
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| show | All show a positive PAS raction (Bright rose)
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| schiff staining after periodate does not demonstrate presence of carbohydrate residues | show 🗑
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| show | PAS reaction
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| show | intensitity of staining in routine PAS reaction is due to the folling factors
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| show | PAS reaction
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| sulfite rinse essential to remove any uncombined leucofuchsin following exposure to schiff's reagent | show 🗑
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| highly chlorinated water is capable of oxidation. if section is transferred directly into tap water, any adsorbed schiff reagent m/b reoxidized to basic fuchsin,which may stain the section | show 🗑
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| for color development, wash in tap water after the sulfite rises | show 🗑
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| show | PAS reaction
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| show | PAS reaction - to determine if there is any previously reactive aldehyde groups present in the tissue
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| show | PAS reaction
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| Liver containing large amounts of glycogen should not be used as control for | show 🗑
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| demonstrate polysaccharides, neutral mucosubstances and basement membranes | show 🗑
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| oxidation of certain tissue elements to aldehydes by periodic acid. | show 🗑
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| show | PAS reaction
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| treat basic fuchsin w/sulfurous acid | show 🗑
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| reduction casues loss of quinoid structures and masking of the chromophores | show 🗑
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| spirochetes and bacteria - brown to black | show 🗑
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| following schiffs reagent, washing in running water causes loss of the bound sulfurous acid group attached at teh centra carbon atom, restoration of quinoid structure in the dye bound tby the aldehyde and the visualization of the typical shiff color | show 🗑
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| Metabisulfate rinses are used to remove excess schiff reagent and prevent false colorization of the tissue elements due to oxidation of adsorbed reagent | show 🗑
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| show | PAS reaction
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| show | PAS reaction
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| show | PAS reaction
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| if demonstrating glycogen use liver containing glycogen or a section of cervix(include both endo and ecto cervix) | show 🗑
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| show | All show a positive PAS raction (Bright rose)
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| schiff staining after periodate does not demonstrate presence of carbohydrate residues | show 🗑
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| show | PAS reaction
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| show | intensitity of staining in routine PAS reaction is due to the folling factors
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| fast green counterstain m/b used instead of hematoxylin. when stain is used to demonstrate fungus | show 🗑
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| show | PAS reaction
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| show | PAS reaction
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| for color development, wash in tap water after the sulfite rises | show 🗑
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| Glutaraldehyde not recommended form fixation, it might react with schiff's reagent | show 🗑
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| a control slide should be run through all steps of the procedure excep the periodate oxidation step | show 🗑
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| chromate-containing fixatives may overoxidize rective groups during fixation the resulting reaction with schiff reagent may be weak | show 🗑
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| Liver containing large amounts of glycogen should not be used as a control, the reaction can be weak but still very apparent | show 🗑
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| reagent problems are apparent if a sectio n of kidney is used a s a control, when the reaction is intended for substances other than glycogen | show 🗑
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| demosntrates glycogen in tissue | show 🗑
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| show | PAS W/diastase
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| Fixation - 10% NBF | show 🗑
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| show | PAS W/diastase
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| control - section of liver containing glycogen, label with and without diastase | show 🗑
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| show | PAS W/diastase
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| show | PAS W/diastase
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| glycogen fixed in picric acid containing fixatives may be more resistant to diastase digestion | show 🗑
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| stratified squamous epi of the ectocervix (glycogen) and glands of the endocervix (mucin) will show a positive schiff reaction on slides without digestion, | show 🗑
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| on slides with digestion, the stratified squamous epi will be negative while the gland of the endocervix remain positive | show 🗑
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| used to differntiate between collagen nd smooth muscle in tumors and identify inreasees in collagenous tissue in disease such as cirrhosis of the liver | show 🗑
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| Thicrhome = three dyes | show 🗑
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| show | Masson trichrome stain
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| show | Masson trichrome stain
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| cytoplasm is less permealbe than collagen, phosphotungsic acid and phosphomolybdic acids cause biebrich scarlet to diffuse out the collagen but not the cytoplasm | show 🗑
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| have numerous acid groups that most likely act as a link between the decolirzed collagen and aniline blue, the collagen dye | show 🗑
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| show | Masson trichrome stain
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| Fixation - bouin is preferred, 10% NBF may be used | show 🗑
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| show | Masson trichrome stain
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| Control - every tissue has internal contorl, no other control sections are needed | show 🗑
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| control - if a control is desired, use uterus, small intestine, appendix or fallopian tube | show 🗑
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| show | Masson trichrome stain
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| show | Masson trichrome stain
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| Collagen and mucus - blue | show 🗑
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| show | Masson trichrome stain
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| show | Masson trichrome stain
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| show | Masson trichrome stain
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| If blue staining of CT tissue appears faded, section has been overdifferentiated in acetic acid | show 🗑
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| altered collagen (burns) may lose affinity for aniline blue and bind to the acid dye instead | show 🗑
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| Picric acid w/less than 10% water is very explosive, its important that solution not be spilled in oven and allowed to evaporate | show 🗑
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| show | Masson trichrome stain
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| iron hematoxylin is used for nuclear staining in trichrome procedure bcuz iron hematoxylin is more resistant than aluminum hematoxylin to decolorization b in acidic dye solution | show 🗑
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| Nuclei - dark blue | show 🗑
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| cytoplasm, keratin, muscle fibers - red | show 🗑
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| show | Masson trichrome stain
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| indentify increase in collagenous CT fibers or differentiate between collagen and smooth muscle fibers | show 🗑
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| show | Gomori one step trichrome
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| phosphotungsic acid favors the red staining of the muscle and cytoplasm. | show 🗑
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| The tungstate ion is taken up by collagen and the CT fiber stain is subsequently bound to this complex, coloring the collagen green or blue, | show 🗑
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| show | Gomori one step trichrome
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| 4 to 5 microns | show 🗑
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| show | Gomori one step trichrome
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| show | Gomori one step trichrome
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| show | Gomori one step trichrome
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| show | Gomori one step trichrome
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| Collagen and mucus - green or blue | show 🗑
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| used to demonstrate pathological changes in elastic tissue | show 🗑
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| demonstrates atrophy of tissue, thinnin/loss that results from arteriosclrotic changes and reduplicarion, breaks/splitting from vascular disease. | show 🗑
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| show | Verhoeff elastic stain
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| show | Verhoeff elastic stain
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| ferric chloride and iodine serve as mordants, they also have oxidizing function that assists in convervting hematoxylin to hematein | show 🗑
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| this method requires that sections be overstained and then differentiated it is REGRESSIVE | show 🗑
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| differentiation is accomplished by using excess mordant, ferrich chloride, to break the tissue-mordant-dye lake complex | show 🗑
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| breaking the tissue-mordant-dye lake complex allows other elements to be decolorized and the elastic fibers to remain stained | show 🗑
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| show | Verhoeff elastic stain
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| Fixation - any well-fixed tissue m/b used. neutral buffered formalin or zenker is preferred | show 🗑
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| 4 to 5 microns | show 🗑
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| control - aorta embedded on edge or cross section of large artery | show 🗑
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| elastic fibers - blue-black to black | show 🗑
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| Nuclei - blue to black | show 🗑
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| show | Verhoeff elastic stain
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| show | Verhoeff elastic stain
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| easy to overdifferntiate this stain | show 🗑
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| show | Verhoeff elastic stain
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| show | Verhoeff elastic stain
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| not necessary to remove mercury deposits, they will be removed by staining solution | show 🗑
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| preparation of van Gieson solution is critical for proper differentiation of muscle and collagen | show 🗑
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| if picric acid is not saturated, collagen will not stain red and cytoplasm, muscle and collagen may all stain the same color | show 🗑
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| show | Verhoeff elastic stain
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| show | Verhoeff elastic stain
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| show | Verhoeff elastic stan
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|
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| Bcuz proper differentiation is sometimes difficult, it is helpful to use duplicate sections differentiated to a slightly different end point | show 🗑
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| used to demonstrate pathological changes in elastic tissue | show 🗑
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| also used to demonstrate normal elastic tissue like identification of veins and arteries to determine if the blood vessels are invaded by tumors | show 🗑
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| Hydrochloric acid and paraldehyde are added to an alcoholic solutin of basic fuchsin to form aldehyde fuchsin | show 🗑
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| show | Aldehyde fuchsin elastic stain
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| show | Aldehyde fuchsin elastic stain
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| Fixation - neutral buffered formalin preferred. Chormate fixatives should be avoided | show 🗑
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| show | Aldehyde fuchsin elastic stain
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| show | Aldehyde fuchsin elastic stain
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| show | Aldehyde fuchsin elastic stain
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| show | Aldehyde fuchsin elastic stain
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| Other tissue elements - Green | show 🗑
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| show | Aldehyde fuchsin elastic stain
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| old solution of aldehyde fuchsin doesnot stain well, staining time may need to be prolonged | show 🗑
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| do not use rosanili, it is nt satisfactory | show 🗑
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| show | Aldehyde fuchsin elastic stain
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| show | Aldehyde fuchsin elastic stain
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| show | Gomori stain for reticular fibers
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| a change from normal reticular fiber pattern, seen in some liver disease | show 🗑
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| show | Gomori stain for reticular fibers
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| show | Gomori stain for reticular fibers
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| show | Gomori stain for reticular fibers
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| show | Gomori stain for reticular fibers
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| toning with gold chloride and removal of unreacted silver with soidum thiosulfate. the final step is a counterstain if desired | show 🗑
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| show | Gomori stain for reticular fibers
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| show | Gomori stain for reticular fibers
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| show | Gomori stain for reticular fibers
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| show | Gomori stain for reticular fibers
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| show | Gomori stain for reticular fibers
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| show | Gomori stain for reticular fibers
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|
||||
| excess of ammonia decrases the sensitivity and results in incomplete impregnation of reticular fibers | show 🗑
|
||||
| show | Gomori stain for reticular fibers
🗑
|
||||
| show | Gomori stain for reticular fibers
🗑
|
||||
| show | Gomori stain for reticular fibers
🗑
|
||||
| the older cleaining method of using a mix of sulfuric acid and potassium dichromate is no longer used bcuz of hazards | show 🗑
|
||||
| show | Gomori stain for reticular fibers
🗑
|
||||
| aka iron alum. | show 🗑
|
||||
| term used for salts that are double or bisulfates, like potassium aluminum sulfate, ammonium aluminum sulfate, chromium potassuim sulfate, and ferric ammonium sulfate | show 🗑
|
||||
| if nuclear fast red is the counterstain, wash the sildes well w/water after staining | show 🗑
|
||||
| if slides are not transfered from counterstain directly to alcohol, they will develop a cloudiness, that can be removed by backing the slide up to water | show 🗑
|
||||
| pattern of reticular fiber staining is very important, should be seen easy w/scanning lens of microscope (liver bx) | show 🗑
|
||||
| show | Gomori stain for reticular fibers
🗑
|
||||
| counterstain, may obscure the easy visualization and therefore should not ve used when visualization of this pattern is important | show 🗑
|
||||
| show | Gomori stain for reticular fibers
🗑
|
||||
| if ammonium hydroxide looses strength, due to loss of ammonia from solution, open a fresh bottle | show 🗑
|
||||
| show | Gomori stain for reticular fibers
🗑
|
||||
| show | Gomori stain for reticular fibers
🗑
|
||||
| show | Gomori stain for reticular fibers
🗑
|
||||
| ammonical silver may form explosive compound, use care in preparation, use and storage of this solution | show 🗑
|
||||
| storage in regrigerator retards formation of explosive compound, avoid exposure to direct sunlight | show 🗑
|
||||
| show | Gomori stain for reticular fibers
🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| are a dx feature of rhabdomyosarcomas or tumors arising form striated muscle. | show 🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| tungsten binds all available hematein to give a blue-colored lake | show 🗑
|
||||
| metal hematein lake stains selected tissue components blue, while the phosphotungstic acid is thought to stain the red-brown components | show 🗑
|
||||
| This stain is referred to as a polychrome stain bcuz one solutin gives two major colors | show 🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| Cross-striations , fibrins - blue | show 🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| Collagen - red/brown | show 🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| you get better results if tissue is fixed in Zenker on in a solution containing mercury | show 🗑
|
||||
| stain is not as good when formalin fixed sections are mordanted as wehn original fixative is mercuric | show 🗑
|
||||
| uneven staining occurs in formalin fixed tissue | show 🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| when this occurs there is failure to show proper density of th blue tones. | show 🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| show | Mallory PTAH
🗑
|
||||
| sodium thiosulfate interferes w/binding of the PTAH, sections should be washe very well after application of sodium thiosulfate | show 🗑
|
||||
| delineates glomerular basement membranes | show 🗑
|
||||
| Methenamine silver demonstrates carbohydrate component of basement membrane by oxidizing the carbs to aldehydes | show 🗑
|
||||
| in silver tecnique for staining basement membrane, silver ions from the methenamine silver complex are first bound to carb compnents of basement membrane and then reduced to visible metallic silver by aldehyde group | show 🗑
|
||||
| toning is done with gold chloride and any unreduced silver is removed by sodium thiosulfate | show 🗑
|
||||
| Fixation - 10% NBF preferred, mercury containing fixatives not recommended | show 🗑
|
||||
| paraffin processed tissue cut at 2 microns | show 🗑
|
||||
| Control - kidney has an internal control. no other control slide is necessary | show 🗑
|
||||
| Basement membrane - black | show 🗑
|
||||
| Background - green | show 🗑
|
||||
| sharper staining of basement membrane and less background staining can be obtained with the use of microwave oven for silver stain | show 🗑
|
||||
| temp is critical and should be below boiling, 95C, immediately after removal from oven. | show 🗑
|
||||
| show | Periodic acid-methenamine silver microwave (PAMS)
🗑
|
||||
| very difficult stain to perform correctly | show 🗑
|
||||
| glomerular basement membrane should appear as continuous black line. stopping silver impregnation too soon results in uneven or interupted stain | show 🗑
|
||||
| application of too much counterstain will mask the silver stain and decrease contrast | show 🗑
|
||||
| demonstrate neutral lipids in frozen sections. | show 🗑
|
||||
| show | Oil red O
🗑
|
||||
| show | Oil red O
🗑
|
||||
| degenarating material containing fat, like cell membranes or myelin, may gro into fat droplets that are demonstrable with fat stain | show 🗑
|
||||
| show | Oil red O
🗑
|
||||
| staining with oil-soluble dye is based on greater solubility of the dye in the lipoid substances than in the usual hydroalcoholic dye solvent | show 🗑
|
||||
| this is a physical method of staining | show 🗑
|
||||
| dyes used must be more soluble in tissue lipid than in the solvent, must not be water soluble, m/b strongly colored, act w/tissue constituents by solution | show 🗑
|
||||
| show | Oil red O
🗑
|
||||
| Fixation 10% NBF or calcium-formal, alcoholic fixatives should not be used, bcz of their lipid dissolving ability | show 🗑
|
||||
| show | Oil red O
🗑
|
||||
| Paraffin sections cant be used bcuz dehydrating and clearing agents dissolve the fat | show 🗑
|
||||
| show | Oil red O
🗑
|
||||
| show | Oil red O
🗑
|
||||
| show | Oil red O
🗑
|
||||
| show | Oil red O
🗑
|
||||
| to improve microtomy of FZ, formalin fixed tissue may be infiltrated with 30% sucrose before freezing | show 🗑
|
||||
| aqueous mounting media must be used, organic solvent present in synthetic resinous media wll disolve fat | show 🗑
|
||||
| show | Oil red O
🗑
|
||||
| if air bubbles are present in section, remove cover slip by soaking the slide in warm water | show 🗑
|
||||
| If glycerine jelly is used for mountin, it should not be overheated, this may melt the fat and displace it | show 🗑
|
||||
| show | Oil red O
🗑
|
||||
| Demonstrates fungus | show 🗑
|
||||
| Control section w/fungs, Pneunmocytis use pneumocytis control | show 🗑
|
||||
| Principle - Polysaccharides in fungal cell wall oxidize to aldehyde by chromic acid | show 🗑
|
||||
| show | Grocott methanamine silver
🗑
|
||||
| show | Grocott methanamine silver
🗑
|
||||
| show | Grocott methanamine silver
🗑
|
||||
| show | Grocott methanamine silver
🗑
|
||||
| chemically cleaned glassware and nonmetallic forceps must be used | show 🗑
|
||||
| Demonstrates spirochetes, hpylori, legionela and cat scratch fever | show 🗑
|
||||
| Principle - argyrophilic method, organisms have ability to adsorb silver ions but not reduce the silver to a visible form, a chemical reducer hydroquinone is used for that purpose | show 🗑
|
||||
| show | Steiner and Steiner
🗑
|
||||
| Background - light yellow | show 🗑
|
||||
| demonstrate spirochetes | show 🗑
|
||||
| Principle - argyrophilic method, spirochetes have ability to bind silver ions but not reduce the silver to a visible form, a chemical reducer hydroquinone is used for this purpose | show 🗑
|
||||
| spirochetes - black | show 🗑
|
||||
| show | Warthin starry
🗑
|
||||
| Background - Pale yellow to light brown | show 🗑
|
||||
| Control - section with spirochetes | show 🗑
|
||||
| indication of reducing substances present in tissue. Melanin, argentaffin granules and formalin pigments w/b stained | show 🗑
|
||||
| show | Schmorl
🗑
|
||||
| The ferrous ions combine w/t ferricyanide present in the staining solution to form a precipitate of ferrous ferricyanide (turnbull blue | show 🗑
|
||||
| Control - seciont w/ melanin or agentaffin granules | show 🗑
|
||||
| show | Schmorl
🗑
|
||||
| show | Bodian
🗑
|
||||
| 6 to 8 | show 🗑
|
||||
| demonstrate nerve fibers and nurofibrils | show 🗑
|
||||
| argyrophil method, requires chemical reduction.Gold chloride, oxalic acid,sodium thiosulfate | show 🗑
|
||||
| is more soluble in the lipids of cell wall than acid alcohol, but is readily removed from bacterias that lack the waxy capsule | show 🗑
|
||||
| show | Kinyoun acid fast stain
🗑
|
||||
| identify mycobacteria | show 🗑
|
||||
| show | carnoy
🗑
|
||||
| lipoid capsule of mycobacteria is of high molecular weight. It is waxy at room temp, successful penetration by aqueous based staining solutions in gram staining is prevented | show 🗑
|
||||
| overcounterstaining w/methylene blue will mask any organisms | show 🗑
|
||||
| wash out acid before counterstaining or tissue will not stain | show 🗑
|
||||
| This method is not satisfactory for mycobacterium leprea | show 🗑
|
||||
| if the section is allowed to dry after carbol fuchsin application, a compound resistant to decolorization w/b formed | show 🗑
|
||||
| show | Ziehl-neelsen method for acid fast bacteria
🗑
|
||||
| lipoid capsule of acid-fast organism takes up carbol-fuchsin and resists decolorization w/dilute mineral acid | show 🗑
|
||||
| acid fast bacteria - bright red, background - light blue | show 🗑
|
||||
| show | Ziehl-neelsen method for acid fast bacteria
🗑
|
||||
| detect the presence of mycobacterium leprea (leprosy) | show 🗑
|
||||
| show | Fite acid-fast stain
🗑
|
||||
| show | Fite acid-fast stain
🗑
|
||||
| fixation any except carnoy | show 🗑
|
||||
| acid fastness of leprosy organism is enhanced when the waxy capsule is protected by the mixutre of peanut oil and xylene. avoid dehydration solutions | show 🗑
|
||||
| show | Auramine-Rhodamine fluorescence
🗑
|
||||
| the exact mechanism of the stain is unknown. both of the dyes use basic dyes that fluoresce at short wave lengths. when both dyes are used toghether, they yield a better stain | show 🗑
|
||||
| show | Carbol fuchsin (kinyoun)
🗑
|
||||
| show | Kinyoun acid fast stain
🗑
|
||||
| identify mycobacteria | show 🗑
|
||||
| show | carnoy
🗑
|
||||
| lipoid capsule of mycobacteria is of high molecular weight. It is waxy at room temp, successful penetration by aqueous based staining solutions in gram staining is prevented | show 🗑
|
||||
| overcounterstaining w/methylene blue will mask any organisms | show 🗑
|
||||
| wash out acid before counterstaining or tissue will not stain | show 🗑
|
||||
| This method is not satisfactory for mycobacterium leprea | show 🗑
|
||||
| if the section is allowed to dry after carbol fuchsin application, a compound resistant to decolorization w/b formed | show 🗑
|
||||
| show | Ziehl-neelsen method for acid fast bacteria
🗑
|
||||
| lipoid capsule of acid-fast organism takes up carbol-fuchsin and resists decolorization w/dilute mineral acid | show 🗑
|
||||
| acid fast bacteria - bright red, background - light blue | show 🗑
|
||||
| show | Ziehl-neelsen method for acid fast bacteria
🗑
|
||||
| show | Fite acid-fast stain
🗑
|
||||
| show | Fite acid-fast stain
🗑
|
||||
| norcadia will also stain with | show 🗑
|
||||
| show | Fite acid-fast stain
🗑
|
||||
| acid fastness of leprosy organism is enhanced when the waxy capsule is protected by the mixutre of peanut oil and xylene. avoid dehydration solutions | show 🗑
|
||||
| show | Auramine-Rhodamine fluorescence
🗑
|
||||
| show | Auramine-Rhodamine fluorescence
🗑
|
||||
| show | Auramine-Rhodamine fluorescence
🗑
|
||||
| show | Auramine-Rhodamine fluorescence
🗑
|
||||
| show | Auramine-Rhodamine fluorescence
🗑
|
||||
| use small amounts of Rhodamine B it will intesify the fluorescence of mycobacteria. too much Rhodamine B will quench fluoresence, even in low concentration | show 🗑
|
||||
| show | Auramine-Rhodamine fluorescence
🗑
|
||||
| Modification of Gram | show 🗑
|
||||
| to demonstrate Gram-negative and Gram-positive bacteria in tissue | show 🗑
|
||||
| crystal violet is applied first followed by an iodine mordant forming a dye lake. | show 🗑
|
||||
| both have a cell wall composed of peptidoglycan | show 🗑
|
||||
| show | Gram-negative and Gram-positive bacteria (Brown-Hopps)
🗑
|
||||
| show | gram-negative bacteria (Brown-Hopps)
🗑
|
||||
| show | large crystal violet-iodine comlex
🗑
|
||||
| can be removed from gram-negative bacteria, bcuz alcohol and acetone disrupt the outer lipopolysaccharide layer | show 🗑
|
||||
| show | Brown-Hopps
🗑
|
||||
| this is a useful method for for screening of infectious agents that cause actinomycosis, nocardiosis, coccidiomucosis, blastomycosis, aspergillosis, rhinosporidiosis and amebiasis | show 🗑
|
||||
| show | Brown-Hopps
🗑
|
||||
| can be used for the demonstration of bacteria, ricketssias and toxoplasma gondii. | show 🗑
|
||||
| show | Modified diff quick Giemsa
🗑
|
||||
| show | Modified diff quick Giemsa
🗑
|
||||
| hpylori - dark blue, other bacteria -blue, nuclei -dark blue, cytoplasm -pink | show 🗑
|
||||
| show | Hotchkiss-Mcmanus Pas reaction
🗑
|
||||
| "carbohydrates". polysaccharides present in fungal cell walls are oxidized by periodic acid to aldehyes. the aldehydes react w/schiffs reagent to yield a rose colored fungi | show 🗑
|
||||
| fungi -rose, background -green | show 🗑
|
||||
| show | Hotchkiss-Mcmanus Pas reaction
🗑
|
||||
| it is helpful to use diastase digestion in sections containing glycogen | show 🗑
|
||||
| oxidizing and schiffs reagent must not be overused or you will get a poor stain | show 🗑
|
||||
| show | Gridley
🗑
|
||||
| this is a modification of Bauer | show 🗑
|
||||
| use chromic acid to oxidize adjacent glycol groups to aldehydes. the aldehydes react w/schiffs reagent. aldehyde fuchsin acts as an aldehyde and occupies uninvolved linkages to schiffs. | show 🗑
|
||||
| is a stronger oxidizing agent than periodic acid. it further attacks and destroys aldehydes | show 🗑
|
||||
| show | Gridley
🗑
|
||||
| show | Gridley
🗑
|
||||
| show | Gridley
🗑
|
||||
| show | dieterle
🗑
|
||||
| show | Steiner and Steiner
🗑
|
||||
| chromic acid is a strong oxidant. it oxidizes many newly released aldehyde groups. it breaks down products that will not react. this helps to suppress the weak background reactions of collagen fibers and basement membranes | show 🗑
|
||||
| fungal cell walls, glycogen and mucins are substances that hvae large quantities of polysaccharide. they remain reactive with methanamine silver reducing it to visible metallic silver | show 🗑
|
||||
| show | Grocott Methanamine-silver nitrate
🗑
|
||||
| show | Grocott Methanamine-silver nitrate
🗑
|
||||
| show | dieterle
🗑
|
||||
| show | Grocott Methanamine-silver nitrate
🗑
|
||||
| Gluteraldehyde should be avoided. Bcuz teh free aldehyde groups reduce the silver and give w/g you nonspecific staining | show 🗑
|
||||
| show | Grocott Methanamine-silver nitrate
🗑
|
||||
| show | Grocott Methanamine-silver nitrate
🗑
|
||||
| show | Grocott Methanamine-silver nitrate
🗑
|
||||
| show | Grocott Methanamine-silver nitrate
🗑
|
||||
| may be used to demonstrate fungi, p Carinii, Actinomyces and related species, Nocardia asteroides and certain encapsulated bacteria | show 🗑
|
||||
| show | Grocott Methanamine-silver nitrate
🗑
|
||||
| show | Grocott Methanamine-silver nitrate
🗑
|
||||
| show | Microwave Methanamine-silver nitrate procedure
🗑
|
||||
| fixation: cytospin -95%alc, F.S. -40%formaldehyde, paraffin sections fxd 10%NBF | show 🗑
|
||||
| show | Microwave Methanamine-silver nitrate procedure
🗑
|
||||
| place plastic coplin jars in loosely closed plastic bag in the microwave oven. any solution that boils over will be left in the bag | show 🗑
|
||||
| show | Microwave Methanamine-silver nitrate procedure
🗑
|
||||
| show | Microwave Methanamine-silver nitrate procedure
🗑
|
||||
| show | Mayers mucicarmine and alcian blue
🗑
|
||||
| the mucoid capsule of cryptococcus neoforma c/b differentiated form other yeast like fungi of similar size and shape | show 🗑
|
||||
| if sections over developed, treat with iodine and sodium thiosulfate for color removal | show 🗑
|
||||
| all bacteria are nonselectively blackened by silver impregnation methods, | show 🗑
|
||||
| demonstrate small, wealky gram negative bacterial such as legionella. | show 🗑
|
||||
| provide much greater sensitivity twhen screening for small numbers of gram pos and gram neg bacteria | show 🗑
|
||||
| demonstrate spirochete and other bacteria in tissue | show 🗑
|
||||
| alipia felis (cat scratch bacillus)-black, legionella pneumophila -black, nocardia asteroides -black, hpylori -black, nuclei brown, erythrocytes -brown | show 🗑
|
||||
| results are more consistent and reliable when staining and developing are done at lowest power levels | show 🗑
|
||||
| show | microwave modification of warthin starry
🗑
|
||||
| show | dieterle
🗑
|
||||
| spirochetes are argyrophilic, the will adsorb silver from a silver solution but the adsorbed silver must be chemically reduced to visible metallic form. Hydroquinone is the reducing agent/developer | show 🗑
|
||||
| QC-tissue containing spirochetes or legionella | show 🗑
|
||||
| considered a primary CT stain, but rarely used. Its an excellent counterstain for other methods such as Verhoeff elastic | show 🗑
|
||||
| show | Van Gieson Picric Acid-Acid Fuchsin Stain
🗑
|
||||
| show | Russell modification of the movat pentachrome stain
🗑
|
||||
| acidic mucosubstances are stained by AB. the alkaline alcohol solution coverts the AB to Moastral fast blue, which is insoluble.Iron hematoxylin is used to stain elastic fibers which are then differentiated w/ferric chloride, | show 🗑
|
||||
| uses potassium ferrOcyanide | show 🗑
|
||||
| Uses potassium ferrIcyanide | show 🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
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Popular Histology sets