Fixatives Chapter One
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each of the black spaces below before clicking
on it to display the answer.
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| 37-40% Formaldehyde, distilled water | show 🗑
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| Hypotonic | show 🗑
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| May produce formalin pigments | show 🗑
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| show | Calcium formalin
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| REcommended to fix and preserve phospholipids | show 🗑
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| 37-40% formaldehyde, ammonium bromide, distilled water | show 🗑
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| acid in nature | show 🗑
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| show | Formalin Ammonium Bromide
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| Lyses RBC's | show 🗑
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| causes nuclei to give a positive schiff's reaction due to the feulgen hydrolysis fixation | show 🗑
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| 37-40% Formaldehyde, Sodium acetate, distilled water | show 🗑
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| This is one of the better formaldehyde solutions to use if one does not want to use a buffered reagent | show 🗑
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| show | 10% Neutralized formalin
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| Solution becomes acidic after withdrawal from the storage bottle | show 🗑
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| show | 10% Neutral Buffered formalin
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| Recommended for routine formalin fixation | show 🗑
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| Hypotonic | show 🗑
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| pH is approximately 6.8 | show 🗑
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| 37-40% Formaldehyde, distilled water, sodium phosphate monobasic, sodium hydroxide | show 🗑
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| show | Modified millonig formalin
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| pH is around 7.2-7.4 | show 🗑
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| has a dual purpose it can alsco act as a fixative for electron microscopy | show 🗑
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| show | Modified millonig formalin
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| 37-40% formaldehyde, absolute ethyl alcohol, distilled water | show 🗑
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| show | Alcoholic formalin
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| Sodium phsophate monobasic, sodium hydroxide, distilled water, gluteraldehyde | show 🗑
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| Make up before using | show 🗑
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| Most frequently used as a fixative for electronmicroscopy as prolonged fixation hardens tissues (two hours or less) | show 🗑
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| is an aldehyde like formalin but it is a dialdehyde | show 🗑
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| show | Gluteraldehyde
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| preserves ultrastructure better than aldehydes | show 🗑
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| Tissues fixed with gluteraldehyde can not be used in stains that use schiff's reagent | show 🗑
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| show | Mercuric chloride
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| is not used alone but is used in compound fixative bcuz it is a very powerful protein coagulant and enhances staining by leaving the tissue very receptive to dyes | show 🗑
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| show | Mercuric chloride
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| show | Mercuric chloride
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| bcuz of hazards associated with it mercury has been replaced by zinc | show 🗑
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| show | Osmium tetroxide
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| show | Osmium tetroxide
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| show | Osmium tetroxide
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| show | Osmium tetroxide
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| only substance that is used as a fixative and a stain | show 🗑
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| coagulant fixative | show 🗑
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| strong enough acid to hydrolyze nucleic acid thereforeit can not be used to perform DNA and RNA stains | show 🗑
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| not a fixative of lipids or carbs | show 🗑
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| decalcify tissue with small calcium deposits such as breast | show 🗑
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| constituent of fixative for glycogen | show 🗑
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| wash out picric acid with 50% alcohol before processing | show 🗑
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| show | Potassium dichromate
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| show | Potassium dichromate
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| chromium will attach to some lipids rendering them inslouble but it does not preserve them | show 🗑
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| Preserves mitochondria but dissolves DNA | show 🗑
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| show | Potassium dichromate
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| show | Zinc Salts
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| show | Zinc Salts
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| Superior nuclear detail and better paraffin infiltration than with formalin alone | show 🗑
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| show | Compound fixative
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| show | Compound fixative
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| show | Compound fixative
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| show | Compound fixative
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| Zenker and Helly | show 🗑
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| Orth | show 🗑
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| show | Compound fixative
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| Zinc formalin | show 🗑
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| show | B-5
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| show | B-5
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| show | B-5
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| Good stain for IHC and most special stains except silver stains | show 🗑
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| picric acid(shrinking effect), 37-40% formaldehyde, glacial cetic acid(swelling effect) | show 🗑
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| lyses RBC and dissolves iron and small calcium deposits | show 🗑
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| show | Bouin
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| Preserves structures with soft and delicate textures | show 🗑
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| Formalin pigments can occur | show 🗑
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| show | Gendre
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| show | Gendre
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| Wash excess picric acid with 80% alcohol | show 🗑
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| Copper acetate, picric acid, 37-40% formaldehyde, distilled water | show 🗑
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| Modification of bouin whic stabilizes RBC membranes and the granules of eosinphils and endocrine cells | show 🗑
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| decalcify small bone specimens | show 🗑
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| more widely used for GI tract bx | show 🗑
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| Mercuric chloride, potassium dichromate, sodium sulfate,distilled water | show 🗑
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| Zenker helly stock solution, Glacial acetic acid | show 🗑
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| Tissue must be treated for mercury pigment if not treated, chromate pigments may also form | show 🗑
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| show | Zenker
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| show | Zenker
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| Good for performing mallory ptah | show 🗑
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| Unsat for silver stains | show 🗑
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| Zenker helly stock solution, 37-40% formaldehyde | show 🗑
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| show | Helly
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| Formalin pigment may also occur | show 🗑
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| preserves erythrocyte | show 🗑
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| show | Helly
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| show | Orth
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| show | Orth
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| used to demonstrate chromaffin granules in the adrenal medulla | show 🗑
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| show | Zamboni buffered picric acid formaldehyde (PAF)
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| show | Zamboni buffered picric acid formaldehyde (PAF_
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| show | Alcoholic zinc formalin
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| Recommended as post fixative after NBF, antigenicity is enhanced and nuclear detail is improved | show 🗑
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| show | Alcoholic zinc formalin
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| show | Unbuffered aqueous zinc formalin
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| show | Unbuffered aqueous zinc formalin
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| show | Unbuffered aqueous zinc formalin
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| absolute ethyl alcohol, chloroform, glacial acetic acid | show 🗑
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| show | Carnoy
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| show | Carnoy
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| show | Rossman
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| similar to gender | show 🗑
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| recommended for carbohydrates particularly glycogen | show 🗑
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| show | Rossman
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| Chromic acid, osmic acid, acetic acid | show 🗑
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| excellent cytoplasmic and chromosomal fixative, especially when the acetic acid is omited | show 🗑
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| show | Flemming
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Created by:
nperez
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