Histology ASCP: Lab protocol and safety
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| Removal of water | Dehydration
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| List dehydrants | Ethyl alcohol, Methyl alcohol, Isopropyl alcohol, Butyl Alcohol, Acetone
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| Reagents that perform both the dehydrating and clearing steps. | Universal solvents
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| List universal solvents | Dioxane, Tertiary Butanol, Tetrahydrofuran
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| Have high index of refraction and will render tissue transparent | Clearing agent
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| Sometimes referred to as dealcoholization agents | Clearing agent
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| List clearing agents | Xylene, Toluene, Benzene, Chloroform, Acetone, Essential oils, Limonene (Xylene substitute), Aliphatic Hydrocarbons (Xylene substitute)
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| Holds the cells and intercellular structures in their proper relationship while thin sections are cut | Infiltrating medium
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| List infiltrating medium | Paraffin, Water-Soluble Waxes (Carbowax), Celloidin, Plastics (Glycol Methacrylate and Epoxy Resins), Agar and Gelatin, 30% Sucrose
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| Better support for hard tissue | Higher melting point
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| Easier to obtain thin sections | Higher melting point
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| Ribboning becomes more difficult | Higher Melting point
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| Poor support for hard tissue | Lower melting point
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| Thin sections are more difficult to obtain | Lower Melting Point
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| Ribboning becomes easier | Lower Melting Point
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| Organic compounds that have the property of binding certain metals | Chelating agents
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| Occulars, or eyepieces | 10x magnification
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| High powered dry lens | 40x to 45x magnification
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| Scanning lens | 2.5x to 4x magnification
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| Intermediate lens | 10x to 20x magnification
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| Oil immersion lens | 90x to 100x magnification
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| Enlarging an object without revealing any increased detail | Empty magnification
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| Using to examine tissue for substances exhibiting the phenomena of double refraction, anisotropism and birefringence | Polarizing microscope
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| Used for the examination of unstained specimens, especially unstained living cells, and allows almost transparent objects to be seen clearly. | Phase-Contrast Microscope
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| Directly transmitted light is excluded and only scattered or oblique light is used | Darkfield Microscopy
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| Used primarily for the study of unstained microorganisms and for silver grains in radioactive staining procedures. | Darkfield Microscope
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| Optical phenomenon in which light of one wavelength is absorbed by a substance and almost instantly re-emitted as a light of a longer wavelength. | Flourescence
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| The specimen either transmits electrons or deflects electrons. A 2D black and white image is seen. | Transmission Electron Microscope
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| A 3D image results as the electron beam sweeps the surface of the specimen and releases secondary electrons. | Scanning Electron Microscope
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| Result when horizontal edges of the block are not parallel | Crooked ribbons
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| Occurs when block is faced too aggressively | Holes in the section
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| Caused by too little blade tilt | Lifting of section from the blade, skipped sections
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| Occurs in hard tissue or overfixed tissue | Washboarding or undulations
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| Occurs in overdehydrated tissue, or by too much blade tilt | Microscopic chatter
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| In Cryostat sections there is a natural tendency for curling and rolling of sections that can be reduced by a: | Antiroll plate
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| In Cryostat sections if a particular tissue tends to split | The temperature may be too low
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| In Cryostat sections if the sections tend to collect at the blade edge | The temperature may be too warm
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| Cryostats are operated at appoximately | -20 degree Celsius for most tissues
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| In Cryostat sections: Brain, liver,spleen, lymph node and endometrial scrapings section better at: | Warmer temperatures
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| In Cryostat sections: fat requires | A much colder temperature
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| Fires involving ordinary combustible materials such as wood, plastics, paper and textiles | Class A Fires
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| Can be extinguished with water or water-based solutions | Class A Fires
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| Fires involving flammable liquids and gases | Class B Fires
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| Requires oxygen to be blocked from the fuel in order to be extinguished | Class B Fires
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| Electrical Fires | Class C Fires
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| Must be extinguished with nonconductive media | Class C Fires
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| Fires of combustible and reactive elements, such as metallic sodium, potassium, magnesium and lithium. | Class D Fires
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| Difficult to control and extinguish because spreading and explosion can occur easily | Class D Fires
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| Health rating | Left Blue Diamond
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| Flammability hazard | Top Right Diamond
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| Reactivity | Right Yellow Diamond
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| Contains special symbols indicating properties and categories | Bottom White Diamond
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