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Histology: Lab
Histology ASCP: Lab protocol and safety
| Question | Answer |
|---|---|
| Removal of water | Dehydration |
| List dehydrants | Ethyl alcohol, Methyl alcohol, Isopropyl alcohol, Butyl Alcohol, Acetone |
| Reagents that perform both the dehydrating and clearing steps. | Universal solvents |
| List universal solvents | Dioxane, Tertiary Butanol, Tetrahydrofuran |
| Have high index of refraction and will render tissue transparent | Clearing agent |
| Sometimes referred to as dealcoholization agents | Clearing agent |
| List clearing agents | Xylene, Toluene, Benzene, Chloroform, Acetone, Essential oils, Limonene (Xylene substitute), Aliphatic Hydrocarbons (Xylene substitute) |
| Holds the cells and intercellular structures in their proper relationship while thin sections are cut | Infiltrating medium |
| List infiltrating medium | Paraffin, Water-Soluble Waxes (Carbowax), Celloidin, Plastics (Glycol Methacrylate and Epoxy Resins), Agar and Gelatin, 30% Sucrose |
| Better support for hard tissue | Higher melting point |
| Easier to obtain thin sections | Higher melting point |
| Ribboning becomes more difficult | Higher Melting point |
| Poor support for hard tissue | Lower melting point |
| Thin sections are more difficult to obtain | Lower Melting Point |
| Ribboning becomes easier | Lower Melting Point |
| Organic compounds that have the property of binding certain metals | Chelating agents |
| Occulars, or eyepieces | 10x magnification |
| High powered dry lens | 40x to 45x magnification |
| Scanning lens | 2.5x to 4x magnification |
| Intermediate lens | 10x to 20x magnification |
| Oil immersion lens | 90x to 100x magnification |
| Enlarging an object without revealing any increased detail | Empty magnification |
| Using to examine tissue for substances exhibiting the phenomena of double refraction, anisotropism and birefringence | Polarizing microscope |
| Used for the examination of unstained specimens, especially unstained living cells, and allows almost transparent objects to be seen clearly. | Phase-Contrast Microscope |
| Directly transmitted light is excluded and only scattered or oblique light is used | Darkfield Microscopy |
| Used primarily for the study of unstained microorganisms and for silver grains in radioactive staining procedures. | Darkfield Microscope |
| Optical phenomenon in which light of one wavelength is absorbed by a substance and almost instantly re-emitted as a light of a longer wavelength. | Flourescence |
| The specimen either transmits electrons or deflects electrons. A 2D black and white image is seen. | Transmission Electron Microscope |
| A 3D image results as the electron beam sweeps the surface of the specimen and releases secondary electrons. | Scanning Electron Microscope |
| Result when horizontal edges of the block are not parallel | Crooked ribbons |
| Occurs when block is faced too aggressively | Holes in the section |
| Caused by too little blade tilt | Lifting of section from the blade, skipped sections |
| Occurs in hard tissue or overfixed tissue | Washboarding or undulations |
| Occurs in overdehydrated tissue, or by too much blade tilt | Microscopic chatter |
| In Cryostat sections there is a natural tendency for curling and rolling of sections that can be reduced by a: | Antiroll plate |
| In Cryostat sections if a particular tissue tends to split | The temperature may be too low |
| In Cryostat sections if the sections tend to collect at the blade edge | The temperature may be too warm |
| Cryostats are operated at appoximately | -20 degree Celsius for most tissues |
| In Cryostat sections: Brain, liver,spleen, lymph node and endometrial scrapings section better at: | Warmer temperatures |
| In Cryostat sections: fat requires | A much colder temperature |
| Fires involving ordinary combustible materials such as wood, plastics, paper and textiles | Class A Fires |
| Can be extinguished with water or water-based solutions | Class A Fires |
| Fires involving flammable liquids and gases | Class B Fires |
| Requires oxygen to be blocked from the fuel in order to be extinguished | Class B Fires |
| Electrical Fires | Class C Fires |
| Must be extinguished with nonconductive media | Class C Fires |
| Fires of combustible and reactive elements, such as metallic sodium, potassium, magnesium and lithium. | Class D Fires |
| Difficult to control and extinguish because spreading and explosion can occur easily | Class D Fires |
| Health rating | Left Blue Diamond |
| Flammability hazard | Top Right Diamond |
| Reactivity | Right Yellow Diamond |
| Contains special symbols indicating properties and categories | Bottom White Diamond |
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