H & E stain
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Stainning to desired intensity and stopping the stain | show 🗑
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| Tissue is overstained,decolorized or differentiated until desired components contrast w/the background | show 🗑
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| Not enough hematoxylin,not stained long enoug,stain overoxidized (old),differentiated too long, bones overdecalcified | show 🗑
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| Hematoxilyn too dark, stained too long, sections too thick, differentiation too short | show 🗑
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| show | Red/brown Nuclei
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| PH of eosin is over 5.0, section too thin, dehydrated too long | show 🗑
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| show | Cytoplasm overstained
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| Metal sheen - unfiltered hematoxylin | show 🗑
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| Dont let slides dry out, keep solutions covered, keep absolute alcohol covered, filter hematoxylin, rinse sections well after ammonia waterso the ph of eason will not become basic, dont dehydrate slides too quickly or the eosin will not be differentialted | show 🗑
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| show | 3 steps of tissue preparation
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| terminate cell metabolism, prevent autolysis, kill viruses, bacteria fungi | show 🗑
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| water soluble | show 🗑
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| alcohol soluble | show 🗑
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| show | nuclear bubbling
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| show | Harris hematoxylin
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| show | Harris hematoxylin (mordant)
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| mercuric oxide | show 🗑
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| sodium iodate m/b used instead of mercuric oxide. (sodium iodate is recommended) | show 🗑
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| (solution A)Ammonium aluminum sulfate, di water, (solution B) hematoxylin, alcohol 95% | show 🗑
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| solution A - saturated ammonium aluminum sulfate, Solution B - hematoxylin, age 3-6 mths and test for readiness | show 🗑
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| show | Delafied hematoxylin mordant
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| oxidation occurs naturally with exposure to light and air. | show 🗑
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| show | Delafied hematoxylin
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| show | Delafied hematoxylin
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| show | Mayer hematoxylin
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| aluminum | show 🗑
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| show | Mayer hematoxylin oxidizer
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| show | Mayer hematoxylin
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| citric acid and chloral hydrate help to prevent scum and precipitates that form | show 🗑
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| hematoxylin, alc 95%, di water, glycerol, ammonium or potassium aluminum sulfate, glacial acetic acid | show 🗑
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| aluminum | show 🗑
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| ripening c/b accomplished naturally or chemically | show 🗑
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| show | Ehrlich hematoxylin
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| show | Gill hematoxylin
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| show | Gill hematoxylin
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| ethylene glycol prevents formation iof surface precipitates | show 🗑
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| sodium iodate is oxidizer and aluminum sulfate is mordant | show 🗑
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| show | Gill hematoxylin
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| show | Gill hematoxylin
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| Solution A - Ferrich chlorided, di water, hydrochloric acid, solution B -hematoxylin, alcohol 95%. | show 🗑
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| show | Weigert hematoxlyin
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| Dye mordant lake is formed | show 🗑
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| show | Weigert hematoxylin
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| show | Weigert hematoxlin
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| show | plasma stains
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| most widely used nuclear stain | show 🗑
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| show | eosin
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| show | lake
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| show | arginine, histidine, lysine
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| show | combine w/eosin. (plasma stain)
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Created by:
nperez
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