H & E stain
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| Stainning to desired intensity and stopping the stain | Progressive Stain
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| Tissue is overstained,decolorized or differentiated until desired components contrast w/the background | Regressive Stain
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| Not enough hematoxylin,not stained long enoug,stain overoxidized (old),differentiated too long, bones overdecalcified | Pale nuclei
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| Hematoxilyn too dark, stained too long, sections too thick, differentiation too short | Nuclei overstained
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| Hematoxylin broken down, slides not blued long enough | Red/brown Nuclei
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| PH of eosin is over 5.0, section too thin, dehydrated too long | Pale staining w/eosin
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| sections stained too long, dehydrated too quickly, eosin concentrated, sections too thick | Cytoplasm overstained
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| Metal sheen - unfiltered hematoxylin | Blue/Black percipitate on slide
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| Dont let slides dry out, keep solutions covered, keep absolute alcohol covered, filter hematoxylin, rinse sections well after ammonia waterso the ph of eason will not become basic, dont dehydrate slides too quickly or the eosin will not be differentialted | H & E helpful hints
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| fixation, infiltration w/paraffin, staining | 3 steps of tissue preparation
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| terminate cell metabolism, prevent autolysis, kill viruses, bacteria fungi | 4 purposes of fixation
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| water soluble | hematoxilyn
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| alcohol soluble | eosin
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| nuclei show coalescense of chromatin | nuclear bubbling
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| hematoxylin, absolute ethyl alc, ammonium aluminum sulfate, Distilled water, Mercuric oxide | Harris hematoxylin
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| Aluminum | Harris hematoxylin (mordant)
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| mercuric oxide | Harris hematoxylin chemical ripening agent
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| sodium iodate m/b used instead of mercuric oxide. (sodium iodate is recommended) | Harris hematoxylin
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| (solution A)Ammonium aluminum sulfate, di water, (solution B) hematoxylin, alcohol 95% | Delafied hematoxylin
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| solution A - saturated ammonium aluminum sulfate, Solution B - hematoxylin, age 3-6 mths and test for readiness | Delafied hematoxylin
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| Aluminum | Delafied hematoxylin mordant
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| oxidation occurs naturally with exposure to light and air. | Delafied hematoxylin
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| alcohol functions to preserve | Delafied hematoxylin
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| glycerol stabilizes solution agains overoxidation and aids in preventing rapid evaporation | Delafied hematoxylin
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| hematoxylin, di water, sodium iodate, ammonium or potassium aluminum sulfate, citric acid and chloral hydrate | Mayer hematoxylin
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| aluminum | Mayer hematoxylin mordant
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| sodium iodate | Mayer hematoxylin oxidizer
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| citric acid is added to adjust the pH | Mayer hematoxylin
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| citric acid and chloral hydrate help to prevent scum and precipitates that form | Mayer hematoxylin
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| hematoxylin, alc 95%, di water, glycerol, ammonium or potassium aluminum sulfate, glacial acetic acid | Ehrlich hematoxylin
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| aluminum | Ehrlich hematoxylin mordant
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| ripening c/b accomplished naturally or chemically | Ehrlich hematoxylin
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| this hematoxylin gives a very sharp nuclear stain and m/b used progressively, more commonly used regressively | Ehrlich hematoxylin
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| di water, ethylene glycol, hematoxylin anhydrous, sodium iodate, aluminum sulfate, glacial acetic acid | Gill hematoxylin
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| Ethylene glycol is excellent solvent for hematoxylin | Gill hematoxylin
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| ethylene glycol prevents formation iof surface precipitates | Gill hematoxylin
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| sodium iodate is oxidizer and aluminum sulfate is mordant | Gill hematoxylin
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| crystillanin hematoxylin m/b used instead of anhydrous hem. | Gill hematoxylin
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| Goblet cells will stain with | Gill hematoxylin
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| Solution A - Ferrich chlorided, di water, hydrochloric acid, solution B -hematoxylin, alcohol 95%. | Weigert hematoxylin
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| Celestine blue m/b substituted for hematoxylin in H&E procedure | Weigert hematoxlyin
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| Dye mordant lake is formed | Weigert hematoxylin
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| iron is the mordant | Weigert hematoxylin
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| used progressively 5 to 30 minutes | Weigert hematoxlin
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| negatively charged - anionic,dyes that combine w/cationic positive charged tissue groups | plasma stains
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| most widely used nuclear stain | hematoxylin
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| most widely used plasma stain | eosin
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| mordant/dye combination | lake
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| basic amino acids | arginine, histidine, lysine
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| arginine, histidine, lysine | combine w/eosin. (plasma stain)
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Created by:
nperez
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