For clinicals in Microbiology dept: Genus B through C
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show | Bordetella pertusis
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Microbes in the genus Bordetella are gram negative or positive? | show 🗑
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Microbes in the genus Bordetella have what shape? | show 🗑
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show | Bordetella are encapsulated
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How would you describe the motility of Bordetella species? | show 🗑
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What class of respiration is exhibited by Bordetella microbes? | show 🗑
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What kind of growth medium should be used when identifying Bordetella microbes? | show 🗑
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Are Bordetella microbes considered intracellular (they invade the host cells) or extracellular (they proliferate in the environment around the host cells)? | show 🗑
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Under the genus "Borrelia," what important species should you know? | show 🗑
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show | Borrelia are gram negative, but stain poorly. So they will stain red or pink.
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Borrelia microbes have what shape? | show 🗑
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Borrelia microbes are encapsulated or unencapsulated? | show 🗑
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How would you describe the motility of Borrelia microbes? | show 🗑
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What class of respiration is exhibited by Borrelia microbes? | show 🗑
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Microbes in the genus Borrelia are __ to culture. | show 🗑
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Borrelia microbes are intracellular or extracellular? | show 🗑
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show | Bordetella stain red or pink because they are gram negative.
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show | Bordetella look like short rods. They are coccobacili.
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Are Bordetella pertusis microbes motile? | show 🗑
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show | Bordetella are extracellular, so they live outside the host cells.
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How is Bordetella pertusis transmitted? | show 🗑
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What diseases are caused by Bordetella pertusis? | show 🗑
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show | 1) Azithromycin 2) Erythromycin 3) Clarithromycin
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show | 1) Pertusis vaccine 2) DPT vaccine
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How do you diagnose Bordetella pertusis in the lab? | show 🗑
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What are the important species under the genus "Brucella?" There are 4. | show 🗑
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Brucella microbes are gram negative or gram positive? | show 🗑
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Brucella microbes have what shape? | show 🗑
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Brucella microbes are encapsulated or unencapsulated? | show 🗑
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show | Brucella are non motile.
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show | Brucella are aerobic, so they breathe oxygen.
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show | Brucella microbes grow on Ruiz Castaneda medium.
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show | Brucella are intracellular microbes.
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T or F: Borrelia burgdorferi microbes stain red. | show 🗑
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Borrelia burgdorferi are __ motile. | show 🗑
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What do Borrelia burgdorgeri require to breath? | show 🗑
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show | Borrelia are hard to culture.
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show | Borrelia is transmitted by ticks which get it from deer and rodents they've bitten.
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show | Lyme disease.
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How do you treat Borrelia burgorferi (or Lyme disease) in the early, prearthritic stages? There are 3. | show 🗑
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Once arthritis manifests in people with Lyme disease (caused by Borrelia burgdorferi) you would treat them with a __ course of antibiotics. | show 🗑
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How do you diagnose Borrelia burgdorferi in the lab? 3 answers. | show 🗑
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show | 1) Direct contact with infected animal 2) Orally, ingestion of unpasteurized milk products.
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show | Brucellosis (duh).
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show | Combination of 1) Doxycycline 2) Streptomycin OR Gentamycin
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show | 1) culture, which is difficult 2) agglutination serology.
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What important species of bacteria fall under the genus "Campylobacter?" | show 🗑
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show | Campylobacter are gram negative, so they stain red or pink.
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show | Campylobacter are spirilla shaped - helical/spiral bacilli with a single polar flagellum.
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How would you describe the motility of Campylobacter? | show 🗑
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show | Campylobacter are microaerophilic, meaning they require lower levels of O2 than is found in the atmosphere. Campylobacter also needs low levels of CO2 (hence, they are capnophilic as well).
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show | Campylobacter grow on blood agar inhibiting other fecal flora - Skirrow's medium or, even better, Preston's agar. Grow at 42°C, the normal avian body temperature, rather than at 37°C, which is normally preferred for bacterial growth. CSM/CAMP
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show | Campylobacter are extracellular bacteria.
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Campylobacter jejuni can be tranferred via the __ route from other mammals and fowl. | show 🗑
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show | meat, especially poultry
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show | drinking contaminated water or dairy products.
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show | Acute enteritis (abdominal pain, diarrhea, fever, and malaise). Bloody stools in severe cases.
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show | No
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How do you diagnose Campylobacter jejuni in the lab? | show 🗑
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The genus Chlamydia/Chlamydophila have what 3 important species of microbe? | show 🗑
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Chlamydia microbes are gram negative or positive? | show 🗑
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What shape are Chlamydia microbes? | show 🗑
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show | Chlamydia are unencapsulated.
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Are Chlamydia microbes motile or nonmotile? | show 🗑
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How do we classify the respiration of Chlamydia microbes? | show 🗑
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Are Chlamydia microbes intracellular or extracellular bacteria? | show 🗑
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show | None. Chlamydia is detected by other means.
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show | No methods for routine use.
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How is Chlamydia pneumoniae transmitted? | show 🗑
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show | Community-acquired respiratory infection.
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show | 1) Doxycycline 2) Erythromycin
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How is Chlamydia trachomatis transmitted? 4 answers. | show 🗑
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show | 1) Nongonococcal urethritis (NGU) 2) Lympnogranuloma venereum (LGV) 3) Trachoma (eyes) 4) Inclusion conjuctivitis of newborn (ICN)
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How is Chlamydia trachomatis treated? 3 answers. | show 🗑
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show | 1) Safe sex 2) Erythromyci of silver nitrate in newborn's eyes.
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show | None.
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How is Chlamydia trachomatis diagnosed in the lab? 3 answers. | show 🗑
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show | Inhalation of dust with secretions or feces from birds, esp. parrots.
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What disease is caused by Chlamydia psittaci? | show 🗑
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show | 1) Tetracycline 2) Doxycycline 3) Erythromycin, though it's less efficient than the others.
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show | 1) Rise in antibody titer 2) Complement fixation 3) Indirect immunofloresence
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show | 1) Clostridium botulinum 2) Clostridium difficile 3) Clostridium perfringens 4) Clostridium tetani
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What color will a Clostridium microbe gram stain? | show 🗑
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How do Clostridum microbes look under the microscope? | show 🗑
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Are Clostridium microbes encapsulated or unencapsulated? | show 🗑
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show | Clostridium are mostly motile.
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How would you classify the respiration of Clostridium organisms? | show 🗑
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show | Clostridium grow best on anearobic blood agar.
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show | Clostridium are extracellular.
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How are Clostridium botulinum microbes spread? | show 🗑
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show | Botulism.
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How do you treat Botulism as caused by Clostridium botulinum? | show 🗑
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show | Proper food preservation techniques.
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How can Clostridium botulinum be diagnosted in the lab? 2 answers. | show 🗑
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How is Clostridium difficile transmitted? 2 answers. | show 🗑
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What disease is caused by Clostridium difficile? | show 🗑
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How can we treat Clostridium difficile? 4 answers. | show 🗑
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How can we detect Clostridium difficule in the lab? 2 ways. | show 🗑
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show | 1) Spores in soil 2) Human flora in vagina and GI tract.
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What diseases are caused by Clostridium perfringens? 3 answers. | show 🗑
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How do you treat gas gangrene (caused by Clostridium perfringens)? 3 ways. | show 🗑
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How do you treat food poisoning caused by Clostridium perfringens? | show 🗑
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What 4 ways are there to diagnose Clostridium perfringens in the lab? | show 🗑
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show | Spores in soil infect wound, burns, or due to surgery.
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show | Tetanus. (Duh)
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What 5 treatments can be used to fight Clostridium tetani, or tetanus? | show 🗑
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How can you prevent tetanus which is caused by Clostridium tetani? | show 🗑
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Diagnosis of Clostridium in the lab is __. | show 🗑
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show | Corynebacterium diphtheriae
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Are Corynebacterium microbes gram positive or gram negative? | show 🗑
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How can the appearance of Corynebacterium microbes be described? | show 🗑
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show | Corynebacterium are unencapsulated.
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What is really distinguishing about the appearance of Corynebacterium under the microscope? | show 🗑
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show | Corynebacterium are nonmotile.
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How do we classify the respiratory features of Corynebacterium? | show 🗑
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What is the best growth medium for Corynebacterium? | show 🗑
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show | Corynebacterium are extracellular.
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How is Corynebacterium diphtheriae transmitted? | show 🗑
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What diseases are caused by Corynebacterium diphtheriae? | show 🗑
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show | 1) Horse serum antitoxin 2) Erythromycin 3) Penicillin
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show | DPT vaccine
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show | Culture on Tinsdale agar, follow with immunologic precipitin reaction.
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