click below
click below
Normal Size Small Size show me how
Clinicals - Micro 1
For clinicals in Microbiology dept: Genus B through C
Question | Answer |
---|---|
Under the genus "Bordetella," what important species should you know? | Bordetella pertusis |
Microbes in the genus Bordetella are gram negative or positive? | Bordetella are gram negative and stain red or pink. |
Microbes in the genus Bordetella have what shape? | Bordetella are small coccobacilli (very short rods which may be mistaken for cocci) |
Are Bordetella microbes encapsulated or unencapsulated? | Bordetella are encapsulated |
How would you describe the motility of Bordetella species? | Bordetella are non-motile |
What class of respiration is exhibited by Bordetella microbes? | Bordetella are aerobic, so they breathe oxygen. |
What kind of growth medium should be used when identifying Bordetella microbes? | Bordetella grow on Regan-Lowe agar |
Are Bordetella microbes considered intracellular (they invade the host cells) or extracellular (they proliferate in the environment around the host cells)? | Bordetella are extracellular |
Under the genus "Borrelia," what important species should you know? | Borrelia burgdorferi |
Microbes in the genus Borrelia are gram negative or gram positive? | Borrelia are gram negative, but stain poorly. So they will stain red or pink. |
Borrelia microbes have what shape? | Borrelia are spirochetes (spiral or corkscrew shaped) |
Borrelia microbes are encapsulated or unencapsulated? | Borrelia are unencapsulated |
How would you describe the motility of Borrelia microbes? | Borrelia are highly motile |
What class of respiration is exhibited by Borrelia microbes? | Borrelia are anaerobic, so they breath CO2. |
Microbes in the genus Borrelia are __ to culture. | difficult |
Borrelia microbes are intracellular or extracellular? | Borrelia are intracellular |
When gram stained, Bordetella pertusis is what color? | Bordetella stain red or pink because they are gram negative. |
Bordetella pertusis look like what under a microscope? | Bordetella look like short rods. They are coccobacili. |
Are Bordetella pertusis microbes motile? | Bordetella are non-motile. |
Do Bordetella pertusis microbes live inside or outside of the host's cells? | Bordetella are extracellular, so they live outside the host cells. |
How is Bordetella pertusis transmitted? | Bordetella is transmitted through respiratory droplets expelled by human hosts. |
What diseases are caused by Bordetella pertusis? | Bordetella causes whooping cough. Bacterial pneumonia is a common secondary infection. (BONUS: In dogs, whooping cough is caused by B. bronchiseptica) |
How do you treat Bordetella pertusis? There are 3. | 1) Azithromycin 2) Erythromycin 3) Clarithromycin |
How do you prevent Bordetella pertusis aside from avoiding respiratory droplets? 2 answers. | 1) Pertusis vaccine 2) DPT vaccine |
How do you diagnose Bordetella pertusis in the lab? | 1) Direct immunoflorescence 2) PCR amplification |
What are the important species under the genus "Brucella?" There are 4. | 1) Brucella abortus 2) Brucella canis 3) Brucella melitensis 4) Brucells suis |
Brucella microbes are gram negative or gram positive? | Brucella are gram negative. They stain red or pink. |
Brucella microbes have what shape? | Brucella are small coccobacilli, so they look like short rods. |
Brucella microbes are encapsulated or unencapsulated? | Brucella are unencapsulated. |
Brucella microbes have what kind of motility? | Brucella are non motile. |
What class of respiration is exhibited by Brucella microbes? | Brucella are aerobic, so they breathe oxygen. |
What kind of growth medium should be used with Brucella microbes? | Brucella microbes grow on Ruiz Castaneda medium. |
Brucella microbes are intracellular or extracellular? | Brucella are intracellular microbes. |
T or F: Borrelia burgdorferi microbes stain red. | True. Borrelia are gram negative and therefore stain red. (They stain poorly, however.) |
Borrelia burgdorferi are __ motile. | highly |
What do Borrelia burgdorgeri require to breath? | CO2 |
How easy is it to culture Borrelia burgdorferi? | Borrelia are hard to culture. |
How are Borrelia burgdorferi microbes transmitted? | Borrelia is transmitted by ticks which get it from deer and rodents they've bitten. |
What disease is caused by Borrelia burgdorferi? | Lyme disease. |
How do you treat Borrelia burgorferi (or Lyme disease) in the early, prearthritic stages? There are 3. | 1) Cephalosporins 2) amoxicillin 3) doxycycline |
Once arthritis manifests in people with Lyme disease (caused by Borrelia burgdorferi) you would treat them with a __ course of antibiotics. | longer |
How do you diagnose Borrelia burgdorferi in the lab? 3 answers. | 1) Microscope with Giemsa or Wright stain 2) PCR 3) Serology, but has a low precision. |
How are Brucella microbes transmitted? | 1) Direct contact with infected animal 2) Orally, ingestion of unpasteurized milk products. |
What disease is caused by Brucella? | Brucellosis (duh). |
How do you treat Brucellosis (as caused by Brucella)? (Hint: Combination of 2, but 3 possible) | Combination of 1) Doxycycline 2) Streptomycin OR Gentamycin |
How do you diagnose Brucella microbes in the lab? | 1) culture, which is difficult 2) agglutination serology. |
What important species of bacteria fall under the genus "Campylobacter?" | Campylobacter jejuni |
Campylobacter microbes are gram negative or gram positive? | Campylobacter are gram negative, so they stain red or pink. |
How would you describe the appearance of Campylobacter microbes? | Campylobacter are spirilla shaped - helical/spiral bacilli with a single polar flagellum. |
How would you describe the motility of Campylobacter? | Campylobacter are motile with a darting motion. |
How would you classify the respiration of Campylobacter? | Campylobacter are microaerophilic, meaning they require lower levels of O2 than is found in the atmosphere. Campylobacter also needs low levels of CO2 (hence, they are capnophilic as well). |
What sort of growth medium should be used with Campylobacter? | Campylobacter grow on blood agar inhibiting other fecal flora - Skirrow's medium or, even better, Preston's agar. Grow at 42°C, the normal avian body temperature, rather than at 37°C, which is normally preferred for bacterial growth. CSM/CAMP |
Campylobacter is intracellular or extracellular? | Campylobacter are extracellular bacteria. |
Campylobacter jejuni can be tranferred via the __ route from other mammals and fowl. | fecal/oral |
Campylobacter jejuni can be transferred from contaminated __, especially __. | meat, especially poultry |
Besides the fecal/oral route, Campylobacter can also be transferred by ? | drinking contaminated water or dairy products. |
What disease can be caused by Campylobacter jejuni? | Acute enteritis (abdominal pain, diarrhea, fever, and malaise). Bloody stools in severe cases. |
Is there a vaccine available for Campylobacter jejuni? | No |
How do you diagnose Campylobacter jejuni in the lab? | Find it in patient feces. |
The genus Chlamydia/Chlamydophila have what 3 important species of microbe? | 1) Chlamydia pneumoniae 2) Chlamydia trachomatis 3) Chlamydophila psittaci |
Chlamydia microbes are gram negative or positive? | Chlamydia are considered gram negative, but are hard to gram stain. |
What shape are Chlamydia microbes? | Round (cocci) or rod (bacillus) shaped. |
Are Chlamydia microbes encapsulated or unencapsulated? | Chlamydia are unencapsulated. |
Are Chlamydia microbes motile or nonmotile? | Motile. |
How do we classify the respiration of Chlamydia microbes? | Chlamydia are facultative aerobes or strictly aerobic. |
Are Chlamydia microbes intracellular or extracellular bacteria? | Chlamydia are obligate intracellular organisms. |
What kind of growth media should be used to grow Chlamydia in the lab? | None. Chlamydia is detected by other means. |
How is Chlamydia pneumoniae diagnosed in the lab? | No methods for routine use. |
How is Chlamydia pneumoniae transmitted? | Respiratory droplets. |
What diseases are caused by Chlamydia pneumonia? | Community-acquired respiratory infection. |
How is Chlamydia pneumoniae treated? There are 2. | 1) Doxycycline 2) Erythromycin |
How is Chlamydia trachomatis transmitted? 4 answers. | 1) Sexually 2) Flies (trachoma) 3) Direct or contaminated surfaces 4) Passage through birth canal. |
What disease can be caused by Chlamydia trachomatis? 4 answers. | 1) Nongonococcal urethritis (NGU) 2) Lympnogranuloma venereum (LGV) 3) Trachoma (eyes) 4) Inclusion conjuctivitis of newborn (ICN) |
How is Chlamydia trachomatis treated? 3 answers. | 1) Azithromycin 2) Erythromycin 3) Tetracyclines like Doxycycline. |
How can you prevent Chlamydia trachomatis? 2 answers. | 1) Safe sex 2) Erythromyci of silver nitrate in newborn's eyes. |
How many vaccines are available for Chlamydia trachomatis? | None. |
How is Chlamydia trachomatis diagnosed in the lab? 3 answers. | 1) Cellular cytoplasmic inclusions by immunoflorescence 2) DNA hybridization 3) ELISA for lipopolysccharides |
How is Chlamydia psittaci transmitted? | Inhalation of dust with secretions or feces from birds, esp. parrots. |
What disease is caused by Chlamydia psittaci? | Psittacosis. (Duh.) |
What treatments work on Chlamydia psittaci? There are 3. | 1) Tetracycline 2) Doxycycline 3) Erythromycin, though it's less efficient than the others. |
How can Chlamydia psittaci be diagnosed in the lab? 3 answers. | 1) Rise in antibody titer 2) Complement fixation 3) Indirect immunofloresence |
Under the genus "Clostridium," what 4 species of microbe are important to know? | 1) Clostridium botulinum 2) Clostridium difficile 3) Clostridium perfringens 4) Clostridium tetani |
What color will a Clostridium microbe gram stain? | Clostridium are gram positive, so they will stain blue or purple. |
How do Clostridum microbes look under the microscope? | Clostridium are large, blunt ended rods. |
Are Clostridium microbes encapsulated or unencapsulated? | Clostridium are normally encapsulated. |
How would you describe the motility of Clostridium? | Clostridium are mostly motile. |
How would you classify the respiration of Clostridium organisms? | Clostridium are obligate anaerobes. |
What kind of growth medium works best with Clostridium microbes? | Clostridium grow best on anearobic blood agar. |
Are Clostridium microbes intracellular or extracellular? | Clostridium are extracellular. |
How are Clostridium botulinum microbes spread? | Spores from soil, aquatic sediments. Contaminated vegs, meat, fish. |
What disease is caused by Clostridium botulinum? | Botulism. |
How do you treat Botulism as caused by Clostridium botulinum? | Antitoxin from horse antiserum. |
How can you prevent infection by Clostridium botulinum? | Proper food preservation techniques. |
How can Clostridium botulinum be diagnosted in the lab? 2 answers. | 1) Mouse inoculation 2) Culture in standard aerobic culture. |
How is Clostridium difficile transmitted? 2 answers. | 1) Spores 2) Human flora - they overgrow other flora when other flora depleted. |
What disease is caused by Clostridium difficile? | Pseudomembranous colitis. |
How can we treat Clostridium difficile? 4 answers. | 1) Stop predisposing antibiotic 2) Fluid and electrolyte replacement 3) Vancomycin if severe or 4) Metronidazole if severe. |
How can we detect Clostridium difficule in the lab? 2 ways. | 1) ELISA for toxin A and B 2) Endoscopy for pseudomembrane. |
How is Clostridium perfringens transmitted? 2 ways. | 1) Spores in soil 2) Human flora in vagina and GI tract. |
What diseases are caused by Clostridium perfringens? 3 answers. | 1) Gas gangrene 2) Acute food poisoning 3) Anaerobic cellulitis |
How do you treat gas gangrene (caused by Clostridium perfringens)? 3 ways. | 1) Debridement or amputation 2)Hyperbaric meds 3) High dose penicillin G or doxycycline |
How do you treat food poisoning caused by Clostridium perfringens? | Supportive care is all that is needed. Disease is self limiting. |
What 4 ways are there to diagnose Clostridium perfringens in the lab? | 1) Microscopically 2) Blood agar culture - forms double-zone B-hemolysis 3) Sugar fermentation 4) Organic acid production |
How is Clostridium tetani transmitted? | Spores in soil infect wound, burns, or due to surgery. |
What disease is caused by Clostridium tetani? | Tetanus. (Duh) |
What 5 treatments can be used to fight Clostridium tetani, or tetanus? | 1) Tetanus immune globulin 2) Horse antitoxin 3) Sedatives 4) Muscle relaxants 5) Mechanical ventilation |
How can you prevent tetanus which is caused by Clostridium tetani? | DPT vaccine. |
Diagnosis of Clostridium in the lab is __. | difficult |
What important species of microbe fall under the genus "Corynebacterium?" | Corynebacterium diphtheriae |
Are Corynebacterium microbes gram positive or gram negative? | Corynebacterium are gram pos, so they are blue or purple. They stain unevenly. |
How can the appearance of Corynebacterium microbes be described? | Corynebacterium are small, pleomorphic rods. (They vary in size and shape depending on conditions.) |
Are Corynebacterium microbes encapsulated or unencapsulated? | Corynebacterium are unencapsulated. |
What is really distinguishing about the appearance of Corynebacterium under the microscope? | Corynebacterium look like palisades, Chinese characters or a picket fence. |
Are Corynebacterium microbes motile or nonmotile? | Corynebacterium are nonmotile. |
How do we classify the respiratory features of Corynebacterium? | Corynebacterium are mostly facultative anaerobes. |
What is the best growth medium for Corynebacterium? | Tinsdale agar. Aerobically? |
Is Corynebacterium are intracellular or extracellular microbe? | Corynebacterium are extracellular. |
How is Corynebacterium diphtheriae transmitted? | Corynebacterium diphtheriae are spread by respiratory droplets and are part of human flora. |
What diseases are caused by Corynebacterium diphtheriae? | Diphtheriae. (Duh.) |
What 3 treatments are available for diphtheriae, which is caused by Corynebacterium diphtheriae? | 1) Horse serum antitoxin 2) Erythromycin 3) Penicillin |
How can you prevent diphtheriae, which is caused by Corynebacterium diphtheriae? | DPT vaccine |
How can you diagnose Corynebacterium diphtheriae in the lab? | Culture on Tinsdale agar, follow with immunologic precipitin reaction. |