UND 363 Muller-mowry colloidal Iron
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| what is M.M. Colloidal Iron often used instead of? | alcian blue PAS
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| purpose of M.M. Colloidal Iron | demonstrate carboxylated and sulfated mucopolysaccharides and glycoproteins
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| what is the ADV. of M.M. Colloidal Iron over alcian blue PAS | more sensitive for acid mucins particularily when they are in small amounts (ie acid mucins in mesotheliomas)
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| principle of M.M. Colloidal Iron | colloidal ferric iron ions absorbed by carboxylated and sulfated mucosubstances because of low PH **iron bound tissue will be shown by the prussian blue reaction**
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| Explain how the prussian blue reaction forms | potassium ferrocyanide treatment- the ferric ions will form ferric ferrocyanide (ie prussian blue)
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| general fixatives of M.M. Colloidal Iron | 10% NBF, Carnoys, alcoholic formalin
AVOID chromatic fixatives (will oxidize polysaccharides ie Zenkers, Hellys, orths)
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| thickness/control for M.M. Colloidal Iron | 4-5micron, sm. bowel, appendix, colon
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| microscope for M.M. Colloidal Iron | Light
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| list the 5 major reagents of M.M. Colloidal Iron | Acetic acid *12%*, colloidal iron, ferrocyanide/hydrochloric acid, water, nuclear fast red
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| what is the function of the 12% acetic acid and colloidal iron in M.M. Colloidal Iron | acetic acid 12% prevents watery dilution of colloidal iron, colloidal iron stains the acidic mucosubstances
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| what does the ferrocyanide/HCL do in M.M. Colloidal Iron | stain carboxylated and sulfated mucosubstances
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| what does the water and nuclear fast red do in the M.M. Colloidal Iron | water washes out the excess reagent, and the nuclear fast red is the counterstain
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| typical results of M.M. Colloidal Iron | acid mucosubstances and sialomucins - dk blue,
nuclei - pink red,
cytoplasm - pink,
cryptococcus - dk blue
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| what can PAS do for M.M. Colloidal Iron | can act as a counterstain (if PAS positive it will stain magenta), and acid/neutral/mixes of mucosubstances will be purple, acid mucosubstances ONLY will be Blue
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| M.M. Colloidal Iron is not specific for what? | acid mucopolysaccharides
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| if the background gets overstained what should be done. | repeat using fresh solutions
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| what other stain will appear visually similar to colloidal iron | alcian blue stain will appear similar to colloidal iron
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