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UND 363 MM Coll FE

UND 363 Muller-mowry colloidal Iron

QuestionAnswer
what is M.M. Colloidal Iron often used instead of? alcian blue PAS
purpose of M.M. Colloidal Iron demonstrate carboxylated and sulfated mucopolysaccharides and glycoproteins
what is the ADV. of M.M. Colloidal Iron over alcian blue PAS more sensitive for acid mucins particularily when they are in small amounts (ie acid mucins in mesotheliomas)
principle of M.M. Colloidal Iron colloidal ferric iron ions absorbed by carboxylated and sulfated mucosubstances because of low PH **iron bound tissue will be shown by the prussian blue reaction**
Explain how the prussian blue reaction forms potassium ferrocyanide treatment- the ferric ions will form ferric ferrocyanide (ie prussian blue)
general fixatives of M.M. Colloidal Iron 10% NBF, Carnoys, alcoholic formalin AVOID chromatic fixatives (will oxidize polysaccharides ie Zenkers, Hellys, orths)
thickness/control for M.M. Colloidal Iron 4-5micron, sm. bowel, appendix, colon
microscope for M.M. Colloidal Iron Light
list the 5 major reagents of M.M. Colloidal Iron Acetic acid *12%*, colloidal iron, ferrocyanide/hydrochloric acid, water, nuclear fast red
what is the function of the 12% acetic acid and colloidal iron in M.M. Colloidal Iron acetic acid 12% prevents watery dilution of colloidal iron, colloidal iron stains the acidic mucosubstances
what does the ferrocyanide/HCL do in M.M. Colloidal Iron stain carboxylated and sulfated mucosubstances
what does the water and nuclear fast red do in the M.M. Colloidal Iron water washes out the excess reagent, and the nuclear fast red is the counterstain
typical results of M.M. Colloidal Iron acid mucosubstances and sialomucins - dk blue, nuclei - pink red, cytoplasm - pink, cryptococcus - dk blue
what can PAS do for M.M. Colloidal Iron can act as a counterstain (if PAS positive it will stain magenta), and acid/neutral/mixes of mucosubstances will be purple, acid mucosubstances ONLY will be Blue
M.M. Colloidal Iron is not specific for what? acid mucopolysaccharides
if the background gets overstained what should be done. repeat using fresh solutions
what other stain will appear visually similar to colloidal iron alcian blue stain will appear similar to colloidal iron
Created by: mustangvxd