UND 363 Alcian Blue Ph 2.5
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| What gives the blue color of alcian blue | show 🗑
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| Is alcian Blue an acidic or basic dye | show 🗑
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| show | carboxylated and sulfated acid mucosubstances
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| At PH 1.0 what are stained with alcian blue | show 🗑
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| show | hyaluronic acid (which preceeds the alcian blue staining in the process)
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| show | NEUTRAL mucins
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| What is the purpose of alcian blue 2.5 | show 🗑
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| what is the principle behind alcian blue 2.5 | show 🗑
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| what are the preferred fixatives for A.B. 2.5 | show 🗑
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| what is the preferred thickness and control specs | show 🗑
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| what is the proper microscope for A.B. 2.5 | show 🗑
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| what are the three major reagents for A.B. 2.5 | show 🗑
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| Acetic acid 3% is used twice in A.B. 2.5 why? | show 🗑
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| show | Alcian blue stains the mucin, nuclear fast red will counterstain
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| show | weakly acidic sulfated mucosubstances, hyaluronic acid and sialomucins will stain dk. blue, Background (nucleus and cytoplasm) will be pink to red
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| show | hydration is important because some alcianophilic structures hydrate slowyly, if not completely hydrated they will stain weakly.
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Created by:
mustangvxd
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