UND 363 Alcian Blue Ph 2.5
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| What gives the blue color of alcian blue | copper
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| Is alcian Blue an acidic or basic dye | basic dye
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| At 2.5 ph what are stained with alcian blue | carboxylated and sulfated acid mucosubstances
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| At PH 1.0 what are stained with alcian blue | ONLY sulfated acid mucosubstances are stained
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| What is used in conjunction with alcian blue to differientated epithelial and connective mucin | hyaluronic acid (which preceeds the alcian blue staining in the process)
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| what mucin will alcian blue not stain | NEUTRAL mucins
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| What is the purpose of alcian blue 2.5 | Demonstrates acid mucopolysaccharides (ie connective tissue mucins *PAS Negative*)
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| what is the principle behind alcian blue 2.5 | forms salt link w/ the acid group of acid mucopolysaccharides
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| what are the preferred fixatives for A.B. 2.5 | 10% NBF or Bouins
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| what is the preferred thickness and control specs | 4-5microns, use colon, sm. intestine, appendix
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| what is the proper microscope for A.B. 2.5 | light
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| what are the three major reagents for A.B. 2.5 | acetic acid 3%, alcian blue, nuclear fast red
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| Acetic acid 3% is used twice in A.B. 2.5 why? | pre alcian blue to protect PH, post alcian blue to prevent non-specific staining
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| what will alcian blue and nuclear fast red do in the A.B. 2.5 tech. | Alcian blue stains the mucin, nuclear fast red will counterstain
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| what are the expected results with A.B 2.5 | weakly acidic sulfated mucosubstances, hyaluronic acid and sialomucins will stain dk. blue, Background (nucleus and cytoplasm) will be pink to red
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| what will incomplete hydration (during deparrafin/hydration steps cause) | hydration is important because some alcianophilic structures hydrate slowyly, if not completely hydrated they will stain weakly.
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