WVSOM Class of 2012 Staining Properties
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| hematoxylin is what kind of stain? | a dark blue or purple dye; anything that stains with hematoxylin is called basophilic
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| eosin is what kind of a stain? | an acidic dye that stains basic components red or pink; structures that stain with eosin are called acidophilic or eosinophilic
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| why do the nuclei in cells stain blue? | because of the heterochromatin (ionized phosphate) that resides within it
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| what color is the nucleolus? | basophilic
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| silver stain is for what kind of tissues? | nervous tissue, reticular fibers (type III collagen)
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| which stain stains glycogen & various carbohydrates with aldehyde? | PAS
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| what is the goal of immunocytochemstry? | visualize some component in a tissue section by means of an antibody
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| what structures does hematoxylin stain? | acidic structures (DNA & RNA)
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| which H & E stain is more specific? | hematoxylin
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| is the ECM basophilic or eosinophilic? | eosinophilic
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| what stain is used for elastic fibers that don't stain well with H & E? | elastic stain
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| elastic stain stains fibers what color? | purple or black
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| which stain is used to stain collagen? | masson's trichrome (also called connective tissue technique)
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| trichrome stain stains nuclei what color? cytoplasm? connective tissue? | the nuclei appear blue, the cytoplasm a bright red, and the connective tissue aqua bluegreen
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| tissues or cells that stain with a silver salt are referred to as what? | argyrophilic
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| PAS stains what color? | dark magenta
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| why do basement membranes stain magenta with PAS? | proteoglycans
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| PAS is commonly used to look at what structure? | bowman's capsule
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| these stains cause tissue to take on a different color than the dye: | metachromic stains
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| what is the most common artifact in slides? | shrinkage
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Created by:
mhassan
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