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une ch 19

cellular bioenergetics: ATP and O2

study high energy bonds in notebook
equation that will represent that ΔG for any reaction can be changed by changing the concentrations of reactants and/or products. ΔG = ΔG0! + RT ln [P]/[S]
nucleoside monophosphokinase refers to adding a phosphate to a NMP
nucleoside diphosphokinase refers to adding a phosphate to a NDP
Be able to write the reaction for adenylate kinase. ADP + ADP <__> AMP + ATP adenylate cyclase transfers one phosphate from a ADP to another.
What kind of reaction is catalyzed by cytochrome P450 monooxygenase? What is oxidized and what is reduced? RH + O2 + NADPH + H+ __> ROH + H2O + NADP+ RH and NADPH are both oxidized. Oxygen is reduced.
Assume a sudden loss of oxygen supply to a cell. Give a simple scenario (sequence of events) that might lead to cell death (necrosis) and release of enzymes. hypoxia-> dec oxidative phosphorylation (due to dec o2 which is the last E acceptor in the ETC)-> dec ATP-> dec active transport-> intracell Ca++ inc and causes cellular swelling->permeability inc (inc CK/troponin in blood) ->cell dies
CK in blood after hypoxia gives us estimate of amt of cell permeability and death
Created by: carolanimal