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Disease Final

Gram(-) Enteric Rods

QuestionAnswer
Humans are the only host for what bug? Shigella
Live in GI tracts of animals, infect humans salmonella, yersinia enterocolitis
Shigella, salmonella, yersinia, vibria considered flora or pathogens? pathogens
anaerobid G(-) bacilli, fementation to generate energy, common fecal org bacteroides fragilis
how are enteric bacilli grouped? ability to ferment glucose
dark blude-black colonies with metallic sheen for vigorous fermentation of lactose produce green pigment for E.coli on what agar? EMB
this agar inhibits G+ orgs, colonies producing glucose fementation show red on .... MacConkey Agar
what does fecal WBC rather than exotoxin induced fluid loss indicate? bacterial invasion of mucosa
G(-) rod, non lactose fermenter, produces H2S salmonella
G(-) rod, non-motile, non lactose fermenter, no H2S, fecal-oral transmission Shigella
what special media is needed to diagnose based on stool culture? one that inhibits G(+) normal flora growth, divides colonies into +/- lactose fermenter
4 y/o M, fecal WBC, occult stool, G(-) rod, nactose non-fermenter, non-motile, oxidase negative, non-spore forming Shigella dysenteriae
mechanism of shigella damage invasion of colonic mucosa, produce enterotoxin
characterized by degeneration of epithelium, inflammation, resulting desquamation/ulceration-> dysenteric stool Shigella
26 y/o AIDS with painful bloody diarrhea. Mac medium: G(-) fac anaerobic rod, ox negative, lactose negative, H2S negative, non-motile shigella flexneri
G(-) rod that produces H2S Salmonella
G(-) rod that ferments lactose E. coli
produces NON bloody diarrhea Vibrio
causes watery diarrhea, up to 1 L/hr, rice-water appearance; oxidase +, G(-) ferments glucose, not lactose Vibrio cholera
3 oxidase negative organisms shigella, E. coli, salmonella
oxidase positive, noninvasive, significant fluid los, no pus, no RBC, no WBC Vibrio cholera
most common type of watery diarrhea in travelers Enterotoxigenic E. cloi
severe acute non-inflammatory watery diarrhea by toxin rather than invasion of epithelium Vibria cholera (enterotoxin stimulates adenylate cyclase)
another name or diarrhea caused by vibrio cholera secretory diarrhea
causes wound infections, gastroenteritis, "primary septicemia", from contaminated seafood, or open wound exposure to seawater Vibrio vulnificus
invasive, affecting primarily the colon, coastal marine saltwater from undercooked searfod (sushi) V. parahaemolyticus
G(-) bacilli caused inflamed leg in 42 y/o alcoholic liver dz, 3 days on the coast celebrating July 4th V. vulnificus
bloody stool, G(-) bacilli, glucose and lactose fermenters, cat+, ox-, purple sheen to EMB Escherichia coli (E. coli O157:H7)
major source of infection in undercooked ground beef, unpasteurized milk/juice, raw sprouts, lettuce, salami E. coli O157:H7
E.coli causes illness from mild intestinal disease to severe kidney complications called hemolytic uremic sydrome
first response is bloody diarrhea, shiga like toxin kills endothelial cells, leading to breakdown of vessel lining caused by E. coli O157:H7
a fastidious lactose non-fermenting organism campylobacter jejuni
Traveler's diarrhea, watery diarrhea due to decreased reabsorption of Na/Cl; increased secretion of Cl/HCO2 into lumen, H2O follows Enterotoxogenic E. coli
Shiga-like toxin, diarrhea with WBCs/RBCs, fever, abd pain, similar to shigellosis Enteroinvasive E. coli
2 selective media that inhibits G(+) growth and aid in ID of G(-) enterics EMB/MacConkey
G(-) bacilli, glucose and lactose fermenter, oxidase negative E. coli
Motile, non-lactose fermenter, produces H2S, capsular virulence factor, from raw eggs, reptile pets, raw milke salmonella
attacks ileocelal region, bacteremia, carrier state, type of fever dz Salmonella typhi
inflammatory diarrhea from chicken; G(-) motile bacilli, ferments glucose, not lactose, Cat-, ox- Salmonella
pt has crampy, periumbilical abd pain, OB negative, fecal eval: green, watery stool, occult negative, negative O&P, WBCs, grown at 42C: G(-) bacilli, oxidase + Campylobacter jejuni
Microaerophilic, fastidious, Ox+, non-lactose fermenter, grows at 42C, common cause infectious/invasive diarrhea Campylobacter
agar plate for lactose-fermenting G(-) pathogens, inhibiting G(+) growth MacConkey's
produced watery diarrhea with cramps, low-grade fever, causative agent in isolated countries Enterotoxogenic E.coli
protozoa causing traveler's diarrhea Giardia duodenalis (giardiasis)
regular component of intestinal flora, standard indicator of fecal pullution, abx-resistant nosocomial pathogen Enterococcus faecalis
cause abx-induced diarrhea or pseudomembranous colitis Clostridium difficile
what is a predominant specied in the colon? anaerobic, G(-) bacilli, BACTEROIDES FRAGILIS
Obligate anaerobe, G(-) rod, common fecal organism, normal flora, low virulence unless necrosis Bacteroides fragilis
G(-) rod, non-enteric, rarely deadly, diarrhea, fecal-oral route, invades cells and lymph, enterotoxin, RBC/WBC in diarrhea Yersinia enterocolitica
microaerophilic, not cultured, urease +, survives in stomach acid by creating ammonia, casue inflam changes -> gastritis; urease breath test can ID this organism Helicobacter pylori
diarrhea, Abx-induced, overgrowth of this organixm, colitis, cramps, fecal leuks, pseudomembranes, toxin is ID'd, not easily cultured Clostridium difficile
Secretory diarrhea, ingest heavily contam meats, poultry, legumes with heart-resistant spores Clostridium perfringens
infantile botulism from Honey Clost. botulinum
Created by: emtdan85