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Micro lab 4
Quiz 4
Question | Answer |
---|---|
coccus, cocci | spheres |
bacillus, bacilli | rods |
vibrios | comma shaped |
spirillum, spirilla | Spirals |
spirochetes | flexible spirals |
What cell shapes can make arrangments | cocci, and baccili |
Cell arrangement is determined by: | by the manner dividing cells hold together and wherther the cells separate after divison |
Diplo- | paired cells |
Strepto- | chains of cells |
tetrad | groups of four cells |
Sarcina | groups of 8 cells |
Staphylo | irregular clusters; grapelike |
To visualize a transparent membrane bound fluid filled bag using a light microscope you need | stain |
solvent and a dye | Stain |
consists of a charged portion (auxochrome) and the colored portioin (chromophore) | Chromogen |
The color portion of the dye is | chromophore |
the charged portion of a stain is | auxochrome |
basic stains have a net charge that is | positive |
On a stain molecule structure the chromophore group is easily identified by the what | cyclic structre |
Many auxochromes are ___ (___) and therefore positively charged (loses OH- or picks up H+) | basic (alkaline) |
Many auxochromes are basic (alkaline) and therefore ___ charged (loses OH- or picks up H+) | positively |
many auxochromes are basic (alkaline) and therefore positely charged; (__ ___ ___ ___ ) | (Losing OH- or picks up H+) |
many bacterial are ___ charged | negatively |
The basic protocol for a simple stain is | smear, heat fix, add stain, wash, blot |
Some examples of Simples stains include | methylene blue, Crystal violet and Safranin |
this type of staining is used to determine the morphology of the bacteria that are to delicate to be heat fixed | Negative stain |
Adding an ___ dye (auxochrome) is repelled by most cells leaving an outline of the cell | acidic dye |
the white outline surrounding the negative bacteria should not be confused with what? | capsule |