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Microbiology Lab 3
Quiz 3 Study Guide
| Question | Answer |
|---|---|
| What things does a medium contain? | -A source of energy/ carbon-glucose -Nitrogen, Sulfur (in the form of amino acids (peptone, beef broth) -Minerals (Na+, Ca2+, Mg2+) -Agar- Polysacharides isolated from sea weed -H2O -Vitamins and growth factors |
| A polysaccharide isolated from sea weed | Agar |
| Minerals important to a Medium? | Na+, Ca+2, Mg+2 |
| Contains nutritional components needed for the growth of many different bacteria | Undefined Media |
| Undefined media is generally used for | Nonfastidious bacteria |
| Contains many of the components found in undefined media, however, specific growth factors, minerals or other compounds are added to facilitate the growth factor of specialized bacteria (Growing specific bacteria) | Defined Media |
| Defined Media is generally used to grow what type of bacteria: | Fastidious Bacteria |
| Very attentive to and concerned about accuracy and detail. | Fastidious Bacteria |
| Types of Bacterial Culturing Techniques include | -Agar Plate -Agar Slant -Liquid Broth |
| What test can be used to obtain a isolated colony of bacteria | Agar Plate (Dilution Streak) |
| What test would you use for specific types of bacteria (pure vs. mixed culture) | Agar Plate |
| What test would you use to differientiate between bacterial species (biochemical differences) | Agar Plate |
| CFU | Colony Forming Unit |
| What test is best used for long term storage and identification purposes (stabs)? | Agar Slant |
| What test is best used for rapid growth of bacteria to provide a uniform inoculum | liquid Broth |
| Color, shape, size and texture of bacterial growth is dependent on; | genetics |
| Color, shape, size and texture of bacterial growth is dependent on genetics, however environment conditions contribute to what: | gene expression |
| Some environmental factors that contribute to gene expression include | -Temperature -Available nutrients -Cell Density |
| The ___ of a colony will allow you to ID the type of bacteria | Morphology |
| Shapes of bacteria growth include | -Circular -Irregular -Punctiform -Filamentous |
| Margins of bacteriua include: | -Entire -Undulated -Lobate -Rhizoid |
| Elevation of bacteria can be described as | -Flat -Raised -Convex -Unbonate |
| The texture of bacteria can be described as: | -Moist -Mucoid -Dry |
| The Pigmentation of a bacteria can be described by identifiying: | (color), opital prpoerties |
| When studying bacteria what Patterns of Bacteria Growth are examined: | Form (shape)---Elevation---Margin |
| When studying bacteria growth patterns in a broth what are you observing for? | Pellicle---Sediment---Turbid---Flocculent |
| Pellicle (broth) | floating |
| Sediment (broth) | formation at the bottom of the broth |
| Turbid (broth) | cloudy |
| Flocculent (broth) | Flake like clumps within the broth |
| Bacterial Growth Patterns that you might expect to see in a slant would include: | Filiform---Arborescent---Spreading---Rhizoid |
| Obligate Aerobes types of bacteria grow best in what type of environment? | O2 rich only |
| These type of bacteria respire aerobically using O2 as the final e- acceptor/oxidizing agents in the ETS | Obligate Aerobes |
| Facultative Anaerobe types of bacteria grow best in what type of environment? | Both O2 rich, and O2 absent |
| These type of bacteria respire aerobically (use O2 as a final e- acceptor) when oxygen is present, but can also use Anaerobic respiration by reducing sulfur or nitrate which is used as a e- accepters instead of oxygen. | Facultative Anaerobes |
| Obligate anaerobes survive best in what type of environment? | O2 absent environment |
| This type of bacteria uses anaerobic respiration, reduces sulfur and nitrate which is used as final e- accepters instead of oxygen | Obligate anaerobes |
| These two type of bacteria are known for causing fermentation | Facilitative Anaerobes, and Obligate Anaerobes |
| This technique uses tryptic soy agar (TSA) prepped in a tube without O2. Oxygen then diffuses into Agar forming a gradient, (aerobic at top, and anaerobinc at the bottom). It is then inoculated with a needle to test the Aerotolerence of the bacteria. | Deep Stab |
| This Aerotolerence test is made of TSA, not completely cooled, then adding the bacteria and mixing, then allowing to cool, and then waiting for bacteria to grow | Agar Shakes |
| This test is done by adding a chemical pack that reduces oxygen to water and generates H2 and CO2. Methylene Blue Indicator strips are added to monitor the oxygen level. The strip is clear when no oxygen is present and blue when oxidized | Anaerobic Jar |
| This test is performed within a typical medium, however it contains sodium thioglycollate (reduces oxygen to water), and a dye that is pink when oxidized and clear when reduced | Fluid Thioglycollate Medium |
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