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Micro lab #2
| Question | Answer |
|---|---|
| What are the genes located on the pGLO plasmid | Beta Lactamase Green flourescent protein ara C regulator protein |
| Incubation on ice | slows fluid cell membranes |
| Transformation solution - CaCl2 | neutralize charges on bacteria to allow plasmid to enter |
| Heat shock @ 42C | increases permiability of cell membrane |
| Incubation with LB broth | allows beta lactamase expression |
| Structure of T even bacteriophage | 1.head 2. tail 3. Nucleic acid 4. capsid 5. collar 6. sheath 7. tail fiber 8. spikes 9. baseplate |
| benefits of phage therapy | kills specific target baceria |
| disadvantages of phage therapy | unknown side effects |
| selective media | will only grow specific bacteria |
| differential media | changes color of colonies making them easier to identify |
| Staphylococcus aureus MSA | yellow fermentation on MSA |
| Staphylococcus epidermidis MSA | growth, non fermentation on MSA |
| S. aureus blood agar | beta - takes color out of blood agar completely |
| S. epidermidis blood agar | gamma - no effect on blood agar |
| S. aureus coagulase test | positive coagulase test |
| S. epidermidis coagulase test | negative coagulase test |
| MacConkeys agar is differential | organisms that ferment lactose turn red on this agar |
| MacConkeys agar is selective because | only gram negative organisms will grow on this agar |
| MacConkeys grows this fermenting organism | E. Coli because it ferments lactose turning pink |
| MacConkeys grows this non-fermenting organism | P. fluorescens |
| sterilization | removing all microbes through heat |
| disinfection | destruction of non endospore forming pathogens on surfaces by chemicals, UV, boiling |
| antisepsis | disinfection of living tissue by use of antiseptic |
| sanitation | lower microbe level to safe level by washing or disinfectant |
| autoclave | sterilization by steam under pressure 15 psi 120C |
| pasteurization | heating at 72C for short time to lower bacteria |
| dry heat | flaming or oven heat for 2 hours @ 170C |
| filtration | air/fluid passed through filter to remove microbes. |
| germicide | kills microorganisms minus endospores |
| bacteriostasis | inhibits growth and multiplication of bacteria |
| Pure Green | silver & citric acid |
| lysol | benzalkonium chloride |
| clorox bleach | Sodium hypochlorite |
| 409 | Alkyl Dimethyl Benzyl Ammonium Chloride |
| Blood agar differential media | certain bacteria produce hemolysins that act on the red cells |
| alpha on blood agar | green around colony |
| beta on blood agar | clear,takes all red color out of agar |
| gamma on blood agar | no effect on agar |
| Gr. A Streptococci bacitracin sensitivity test | any inhibition zone with "A" disc |
| Strepticocci pneumonia optochin test | P disc inhibition |
| Alpha hemolytic Strepticoci pneumonia optochin test | no inhibition, negative |
| normal flora nose | staphylococcus epidermidis |
| normal flora throat | Alpha hemolytic strepticocci |
| broad spectrum antibiotic | works against both gram - and gram + bacteria. SXT is an example. |
| bacteriocidal | kill growing microbes directly |
| bacteriostatic | stop microbes from growing |
| Modes of action Penicillin | Modes of action Inhibits cell wall synthesis |
| Mode of action neomycin | Mode of action Inhibits protein synthesis |
| Mode of action nalidixic acid | Mode of action Inhibits nucleic acid synthesis |
| Mode of action sulfamethoxazole | Mode of action inhibits metabolism |
| Sensitive | antibiotic inhibits or kills growth |
| Resistant | antibiotic does not inhibit growth |
| Intermediate | not resistant, but not sensitive. Can be used when a sensitive is not an option. |
Created by:
jhlcc
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