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BacterialGrowthGene
lecture 22 alto
| Question | Answer |
|---|---|
| deletion mutant | organism with the type of mutation characterized by the loss of a portion of its genome |
| best environment in which microaerophiles grow | grow best in low O2 tension but can tolerate normal atmospheric levels, possess SOD but NOT catalase |
| autotrophs | derive energy from oxidizing inorganic substrates |
| heterotrophs | use organic carbon components as energy (include all medically important bacteria) |
| characteristics of log phase | cells are large, rapidly growing and thus susceptible to agents that target cell division steps, rich in ribosomes and metabolically active |
| characteristics of stationary phase | cells are usually smaller, less active metabolically and more reistant to toxic agents |
| start codon | ATG |
| fever kills mesophiles (optimal temp is 37 deg C) | |
| direct measurement method of bacterial growth | hemocytometer (slide with grid for counting bacteria) |
| indirect measurement method of bacterial growth | turbidity of broth, bacterial dry weight |
| semi-diploid or merodiploid | occurs when a copy of just one single gene is inserted into a bacterium via plasmid or other methods |