ASCP Histology Tech: Processing/Instrumentation
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| show | alcohol, acetone, dioxane, tetrahydrofuran (last two are universal solvents)
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| show | mushy soft sections
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| show | ethanol (dehydratant)
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| __________ cannot be used in processors or with isopropanol | show 🗑
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| show | dehydratant and paraffin
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| show | benzene (clearnat)
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| show | 53 degrees celsius
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| one disadvantage using dioxane for processing is | show 🗑
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| one xylene disadvantage is | show 🗑
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| show | 55 to 58 degrees C
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| show | ethanol (dehydratant)
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| show | cracking of the block
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| show | serial sections are easy to obtain
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| when cutting a block, if the tissue seems hard and brittle... | show 🗑
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| show | thick
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| when used for clearing, cedar wood oil must be followed by | show 🗑
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| show | formation of large crystals
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| show | using water-soluble wax (embedding medium)
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| show | BEST DEHYDRATANT!!!
PEL=1,000 /
60-65-95-95-absolute
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| show | ethanol + methanol or ethanol + isopropanol (dehydratant)
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| show | POISONOUS dehydratant, NOT USED ALONE PEL=200 / not for skin; breaks down to formaldehyde; causes blindness and death
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| Dioxane | show 🗑
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| show | a dehydratant that CANNOT BE USED IN PLACE OF ETHANOL WHEN STAINING! PEL=400
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| show | (dehydratant) PEL-OSHA=1,000 / PEL-NIOSH=250 / (rapid, cheap, but absorbs water from the air) USED IN TOUCH PREPS OR FAST FIXES. CAN ALSO BE USED LIKE A UNIVERSAL SOLVENT.
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| BUTYL ALCOHOL | show 🗑
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| show | (dehydratant)odorous, expensive, solidifies at room temperature,MIXES WITH WATER, ETHANOL, XYLENE / PEL=100
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| Tetrahydrofuran | show 🗑
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| PEL=100 / used as clearing agent in most labs | show 🗑
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| Toluene | show 🗑
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| show | fast, does not over-harden, WILL HARDEN MUSCLE, TENDON, UTERUS more than TOLUENE // PEL=10
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| show | ALIPHATIC HYDROCARBONS AND LIMONENE
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| CHLOROFORM PEL=50 | show 🗑
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| show | CARBOWAX: dehydrating and clearing unnecessary. Infiltrates tissue directly from fixative.BAD: floating out occurs
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| show | celloidin
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| great for undecalcified bone, kidney, bone marrow, lymph node biopsies | show 🗑
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| 5% aqueous formic acid will | show 🗑
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| show | dehydrated
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| ethylene glycol monoethyl ether | show 🗑
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| sections processed by _______ method show less shrinkage than when processed by paraffin. | show 🗑
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| best processing for enzyme demonstration | show 🗑
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| ion exchange resin & electrolytic methods of decalcification are considered | show 🗑
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| show | chelating agents
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| 30% sucrose | show 🗑
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