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MICROBIO Ex20

Selective and Differential Media

QuestionAnswer
This agar is very selective due to the high concentration of salt it contains. Mannitol salt agar.
Used to isolate Gram-positive Staphylococcus Mannitol salt agar.
PEA? Negative No or slight growth -> Gram-negative because organism inhibit by phenylethyl alcohol
PEA? Positve Moderate to abundant of growth -> Gram-positive because organism is not inhibited by phenylethyl alcohol.
EMB medium - how to know if the organism is Gram - positive? No growth or light growth without changing color of growth -> Gram-positive because organism is inhibited by eosin and methylene blue.
EMB medium - how to know if the organism is non-coliform Gram-negative Moderate or abundant of growth, and growth doesn't change color ->Gram - negative noncoliform. because organism is inhibited by eosin and methylene blue, organism is nonfermenter.
EMB medium - how to know if the organism iscoliform Gram-negative Moderate or abundant of growth with pink to metallic green color -> Gram-negative coliform. Because organism is inhibited by eosin and methylene blue, organism ferment sugar to acid.
PEA Phenylethyl Alcohol - used to isolate coliform Gram-positive organisms
EMB Eosin Methylene Blue - used to isolate Gram-negative organisms.
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Created by: pipi1402
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