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Reagent uses

QuestionAnswer
Weigert stains nuclei
mucicarmine epithelial mucins
metanil yellow conunterstain
Periodic Acid oxidizer
Hematoxylin stains nuclei counter stain - Alcian Blue PAS
Phosphate buffer pH 6.0 dissolve diastase
diastase digests glycogen
schiffs stains glycogen - neutral mucins
Acetic Acid Sensitizer
Acican Blue acid mucins
NFR counterstain
80% Alcohol NaCL Cleaner backgroun
Congo Red amyloid
Crystal Violet amyloid stains gram positive organisms - Gram
aqueous mounting media hold stain used to cover slip - Oil Red O
Lugol iodine mordant/oxidizer
10% Ferric Chloride mordant/oxidizer
5% alcoholic hematoxylin stains elastic fibers
2% Ferric Chloride differentiator
Sodium Thiosulfate removes excess stain - EVG removes unreduced/unreacted silver - Retic & GMS & Bielschowsky oxidizer - PTAH
Van Gieson counterstain
Bouins mordant
Beibrich Scarlet Acid Fuchsin muscle & cytoplasm
Phosphomolybidic/Phosphotungstic Acid differentiator/decolorizer
analine blue stains collagen
acetic acid solution differentiator
Potassium permanganate oxidizer
Potassium metabisulfite removes excess Potassium permanganate
Ferric Ammonium Sulfate sensitizer
ammonical silver solution stains retic fibers
formaldehyde 37-40% reduces silver
gold chloride toner
Zenker primary Fix
Gram Iodine removes mercuric pigment Mordant - Gram
oxalic Acid Bleach
PTAH Solution muscle & skeletal fibers
Safranin O Gram neg. organisms
Tartazine counterstain
Cellosolve or Gallego Differentiator/Decolorizer
Carbol Fuchsin (basic fuschin, phenol crystals, alcohol) stains AFB bacteria
Acid Alcohol differentiator
Methylene Blue counterstain
Light Green Counterstain
4-5% Chromic Acid oxidizer
silver nitrate - methenamine silver - Na Borate Solution stains fungus
Uranyl Nitrate Sensitizer
Silver Nitrate stains organism - Steiner stains nerve fibers & senile plaques - Bielschowsky
Gum mastic Protects organism (resin)
Developer (reducing) solution Gum mastic + absolute alcohol + hydroquinone (filter)+ silver solution
Propylene Glycol or isopropanol solvent to dissolve dye
Oil Red O stains neutral fats
Luxol Fast Blue stains myelin (24 hours)
Lithium Carbonate differentiator
70% alcohol differentiator
Formaldehyde part of developer
Created by: mistysenter21
Popular Histology sets

 

 



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