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Immunofluorescence
Detection of antinuclear auto-antibodies
| Question | Answer |
|---|---|
| FITC | Fluorescein isothiocyanate |
| What induces autoimmune diseases? | Self-reacting antibodies, genetic disposition, hormones, aging, infections, trauma, drugs. |
| A hallmark of autoimmune diseases? | Presence of auto-reactive antibodies directed against antigen(s). |
| FITC | Fluorescein isothiocyanate |
| SLE | Systemic lupus erythematosus |
| A hallmark of autoimmune diseases? | Auto-reactive antibodies that target antigen(s), causing organ or system malfunction. |
| A standard method for detecting antinuclear autoantibodies? | Indirect immunofluorescence |
| Indirect immunofluorescence materials | Tissue sections from rat kidney or liver (antigen). Human serum as primary antibody. Fluorescence labeled or secondary antibody for detection. |
| What produces different staining patterns? | Antinuclear autoantibodies with different nuclear targets |
| Homogenous nuclear staining is caused by? | Targeting of DNA-nucleoprotein histone complexes |
| Peripheral staining of nuclear membrane is caused by? | Antibodies against DNA. |