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Clinical Micro 1

TermDefinition
Staphylococcus aureus Catalase: pos Coagulase: pos Trehalose: pos Mannitol: pos Maltose: pos Sucrose: pos Mannose: pos Beta hemolysis Smooth, creamy yellow colonies Gram pos cocci in clusters
Staphylococcus saprophyticus Catalase: pos Coagulase: neg Urea: pos Novobiocin: resistant Trehalose: pos Mannitol: delayed pos Maltose: pos Sucrose: pos Mannose: neg No hemolysis Glossy, smooth, convex, white/yellow colonies Gram positive cocci in clusters
Staphylococcus epidermidis Catalase: pos Coagulase: neg Urea: pos Novobiocin: susceptible Trehalose: neg Mannitol: neg Maltose: pos Sucrose: pos Mannose: delayed pos No hemolysis Glossy, smooth, convex, white/yellow colonies Gram pos cocci in clusters
Micrococcus spp. Catalase: pos Coagulase: neg Microdase: pos Bacitracin: susceptible No hemolysis Opaque, convex, white/yellow/tan colonies Gram positive cocci in tetrads
Streptococcus pyogenes Catalase: neg Motile: neg PYR: pos CAMP: neg Beta hemolysis Gram positive cocci in chains
Streptococcus agalactiae Catalase: neg Motile: neg PYR: neg CAMP: pos Beta hemolysis Gram positive cocci in chains
Streptococcus pneumoniae Catalase: neg Motile: neg Optochin: susceptible Alpha hemolysis Gram positive lancets singly or diplococci
Alpha Viridans streptococci Catalase: neg Motile: neg PYR: pos Optochin: resistant
Enterococcus spp. Catalase: neg Motile: neg Bile esculin: pos Alpha/gamma hemolysis Gram positive cocci singly or in pairs
Bacillus anthracis Catalase: pos No hemolysis Dry, irregular, round glass colonies Gram pos bacilli Inhalation of endospores/exposure to animals/food-borne illness
Bacillus cereus Catalase: pos No hemolysis Dry, irregular, round glass colonies Gram pos bacilli Exposure to soil/food borne illness/possible GI/eye infections
Corynebacterium spp. Catalase: pos Gray/white colonies Gram positive bacilli in palisades diphtheriae: brown halo on tellurite agar
Listeria monocytogenes Catalase: pos Motile: pos, umbrella pattern VP: pos CAMP: pos Bile esculin: pos Ferments glucose Narrow zone of beta hemolysis Gram positive rods, possible in chains
Arcanobacterium Catalase: neg Gelatinase: neg Gram positive rods, curved with pointed ends
Lactobacillus Catalase: neg Gram positive rods in long chains, pleomorphic
Erysipelothrix Catalase: neg TSI: H2S Gram positive short and long rods
Gardnerella vaginalis Catalase: neg Beta hemolysis Small, pleomorphic gram variable or gram negative coccobacilli Small, gray, opaque colonies
Trueperella Catalase: neg Gelatinase: pos Gram positive rods with pointed ends, irregular
Haemophilus influenzae Gram negative diplococci or rods Unencapsulated: small, smooth, translucent colonies Encapsulated: larger, mucoid colonies with mousy odor No hemolysis X and V factor
Haemophilus parainfluenzae Gram negative diplococci or rods large, rough, serrated edges, wrinkled, off-white to yellow colonies V factor only
Haemophilus ducreyi Gram negative diplococci or rods Small, flat, gray, smooth, translucent to opaque colonies Slight beta hemolysis X factor only
Haemophilus aegyptius Gram negative diplococci or rods Small, flat, smooth, convex, translucent colonies X and V factor
Neisseria gonorrhoeae gram negative diplococci Glucose: pos Lactose: neg Maltose: neg Sucrose: neg Small, gray/white, convex, translucent, smooth or irregular colonies
Neisseria meningitidis gram negative diplococci Glucose: pos Lactose: neg Maltose: pos Sucrose: neg Medium, smooth, round, moist, gray to white colonies
Neisseria lactamica gram negative diplococci Glucose: pos Lactose: pos Maltose: pos Sucrose: neg Small, nonpigmented or yellowish, smooth, transparent colonies
Neisseria sicca gram negative diplococci Glucose: pos Lactose: neg Maltose: pos Sucrose: pos Large, nonpigmented, wrinkled, coarse and dry colonies
Moraxella catarrhalis gram negative diplococci Oxidase: pos Glucose: pos Lactose: neg Maltose: pos Sucrose: pos Large, pinkish to brown, opaque, smooth, friable, hockey puck colonies
Asaia spp. Oxidase: pos Pale and pink colonies Do not fluoresce
Eikenella corrodens Oxidase: pos Moist, clear centers with flat, spreading growth colonies May pit the agar surface Bleach odor
Weeksella virosa Oxidase: pos Mucoid, tan to brown colonies
Bergeyella zoohelcum Oxidase: pos Sticky, tan to yellow colonies
Methylobacterium spp. Oxidase: pos Pink to coral colonies Dark pink under UV light
Escherichia coli Gram negative rods Catalase: pos Oxidase: pos Glucose: pos Nitrate: pos Lactose: pos Sorbitol: pos
Escherichia coli O157:H7 Gram negative rods Catalase: pos Oxidase: pos Glucose: pos Nitrate: pos Lactose: pos Sorbitol: neg
Plesiomonas shigelloides Gram negative rods Catalase: pos Oxidase: pos Glucose: pos Nitrate: pos Indole: pos Motile: pos Lactose: neg Lysine: pos Ornithine: pos Arginine: pos
Salmonella Enteritidis Gram negative rods Catalase: pos Oxidase: pos Glucose: pos Nitrate: pos Indole: neg MR: pos Lactose: neg Sorbitol: pos H2S: pos Citrate: pos LDC: pos ODC: pos
Salmonella Typhi Gram negative rods Catalase: pos Oxidase: pos Glucose: pos Nitrate: pos Indole: neg MR: pos Lactose: neg Sorbitol: pos H2S: pos Citrate: neg LDC: pos ODC: neg
Shigella sonnei Gram negative rods Catalase: pos Oxidase: pos Glucose: pos Nitrate: pos Indole: neg Lactose: neg MR: pos Citrate: neg ODC: pos ONPG: pos KIA: K/A
Shigella spp. Gram negative rods Catalase: pos Oxidase: pos Glucose: pos Nitrate: pos Indole: neg Lactose: neg MR: pos Citrate: neg ODC: neg ONPG: neg KIA: K/A
Yersinia pestis Gram negative rods Catalase: pos Oxidase: pos Glucose: pos Nitrate: pos MR: pos Citrate: neg ODC: neg Urea: neg ONPG: pos Rough, cauliflower like colonies Safety pin staining
Yersinia enterocolitica Gram negative rods Catalase: pos Oxidase: pos Glucose: pos Nitrate: pos MR: pos Citrate: neg ODC: pos Urea: pos ONPG: pos Bulls eye colonies Safety pin staining
Citrobacter freundii Indole: neg MR: pos Citrate: pos Lactose: pos ODC: neg H2S: pos
Citrobacter koseri Indole: pos MR: pos Citrate: pos Lactose: pos ODC: pos
Edwardsiella tarda Indole: pos MR: pos H2S: pos Urea: neg LDC: pos ODC: pos ONPG: neg Sorbitol: neg
Enterobacter spp. Lactose: pos KIA: A/A gas ODC: pos VP: pos Citrate: pos Urea: weak pos Smooth, convex, gray colonies Non hemolytic
Klebsiella pneumoniae Indole: neg Lactose: pos VP: pos Citrate: pos Urea: pos ODC: neg Decarboxylate lysine: pos Mucoid colonies
Klebsiella oxytoca Indole: pos Lactose: pos VP: pos Citrate: pos Urea: pos ODC: neg Decarboxylate lysine: pos Mucoid colonies
Proteus mirabilis Indole: neg MR: neg VP: pos Citrate: pos PAD: pos Swarming colonies
Proteus vulgaris Indole: pos MR: pos VP: neg Citrate: neg PAD: pos Swarming colonies
Morganella morganii susp. morganii Indole: pos MR: pos VP: neg Citrate: neg PAD: pos
Providencia spp. Indole: pos MR: pos VP: neg Citrate: pos PAD: pos
Pseudomonas aeruginosa Gram negative bacilli Lactose: neg Catalase: pos Oxidase: pos Motile: pos Glucose: pos Xylose: pos Mannitol: pos ADH: pos LDC: neg ODC: neg Gelatin: neg Nitrate: pos Beta hemolysis Mucoid colonies
Pseudomonas fluorescens Gram negative bacilli Lactose: neg Catalase: pos Oxidase: pos Motile: pos Glucose: pos Xylose: pos ADH: pos LDC: neg ODC: neg Gelatin: pos Nitrate: neg Beta hemolysis
Pseudomonas putida Gram negative bacilli Lactose: neg Catalase: pos Oxidase: pos Motile: pos Glucose: pos Xylose: pos ADH: pos LDC: neg ODC: neg Gelatin: neg Nitrate: neg Beta hemolysis
Pseudomonas stutzeri Gram negative bacilli Lactose: neg Catalase: pos Oxidase: pos Motile: pos Glucose: pos Xylose: pos Mannitol: pos ADH: neg LDC: neg ODC: neg Gelatin: neg Nitrate: pos Beta hemolysis
Burkholderia cepacia Gram neg bacilli Lactose: neg Oxidase: weak pos Motile: pos Glucose: pos Xylose: V Mannitol: pos ADH: pos LDC: V ODC: neg Nitrate: V Gelatin: V Dirt smell
Burkholderia pseudomallei Gram neg bacilli Lactose: neg Oxidase: pos Motile: pos Glucose: pos Xylose: pos Mannitol: pos ADH: pos LDC: neg ODC: neg Nitrate: pos Gelatin: pos Earthy odor
Burkholderia mallei Gram neg bacilli Lactose: V Oxidase: weak pos Motile: pos Glucose: pos Xylose: V Mannitol: neg ADH: pos LDC: neg ODC: neg Nitrate: pos Gelatin: neg
Actinobacillus spp. Short, gram negative rods singly, in pairs, or in chains Bipolar staining Catalae: neg Oxidase: pos Urea: pos Bile esculin: neg
Aggregatibacter spp. Pleomorphic gram negative bacilli Catalase: pos Indole: neg Oxidase: pos Urea: pos Bile esculin: neg
Cardiobacterium spp. Pleomorphic gram negative rods Catalase: neg Indole: pos Oxidase: pos Nitrate: neg Slight alpha hemolysis May pit agar
Kingella spp. Short, plump, gram negative coccobacilli with square ends Catalase: neg Indole: neg Oxidase: pos Nitrate: neg Beta or gamma hemolysis May pit agar
Capnocytophaga spp. Gram negative fusiform bacilli, tapered end Catalase: neg Oxidase: neg Flat colonies with hazy edges
Acinetobacter spp. Gram negative plump coccobacilli Catalase: pos Oxidase: neg Motile: neg Lactose: neg Smooth, opaque, raised, creamy colonies Purple pigmentation on MAC
Bordetella spp. Gram negative coccobacilli or rods Lactose: neg
Stenotrophomonas maltophilia Gram negative rods Catalase: pos Oxidase: neg Motile: pos Lactose: neg Decarboxylase lysine: pos Gelatin: pos DNAse: pos Nitrate: V Large, smooth, glistening, uneven edges, lavender-green to light purple pigmented colonies Ammonia odor
Achromobacter spp. Gram negative bacilli Oxidase: pos Motile: pos Glucose: pos Xylose: pos Lactose: neg Nitrate: pos
Alcaligenes spp. Gram negative rods or coccobacilli Catalase: pos Oxidase: pos Motile: pos Urea: neg Glucose: pos Lactose: neg Nitrate: neg Gelatin: V Green apple odor
Shewanella putrefaciens Gram negative bacilli Oxidase: pos Glucose: V Lactose: neg Nitrate: pos TSI: H2S Brown/Tan pigment
Ochrobactrum spp. Gram negative bacilli Oxidase: pos Glucose: pos Xylose: pos Mannitol: pos Lactose: neg Nitrate: pos Beta galactosidase: neg
Rhizobium spp. Gram negative bacilli Oxidase: pos Glucose: pos Xylose: pos Mannitol: pos Lactose: neg Nitrate: pos Beta galactosidase: pos Red/orange pigment
Campylobacter coli Curved, seagull shaped, gram negative rods Catalase: pos Oxidase: pos Hippurate: neg Cephalothin: resistant Naladixic acid: suceptible
Acrobacter spp. Curved gram negative rods Catalase: pos Oxidase: pos Urea: neg
Helicobacter spp. Curved gram negative rods Catalase: pos Oxidase: pos Urea: pos Nitrate: pos
Campylobacter fetus Curved, seagull shaped, gram negative rods Catalase: pos Oxidase: pos Hippurate: neg Cephalothin: susceptible Naladixic acid: resistant
Campylobacter jejuni Curved, seagull shaped, gram negative rods Catalase: pos Oxidase: pos Hippurate: pos Cephalothin: resistant Naladixic acid: susceptible
Elizabethkingia meningoseptica Gram negative bacilli Oxidase: pos Indole: pos Motile: neg Glucose: pos Mannitol: pos Lactose: neg ONPG: pos Gelatin: pos Bile esculin: pos Yellow colonies Fruity odor
Moraxella catarrhalis Gram negative coccobacilli Oxidase: pos Indole: pos Motile: neg Glucose: pos Lactose: neg Yellow colonies
Vibrio Gram negative rods Oxidase: pos Indole: pos Lactose: neg LDC: pos
Aeromonas Gram negative rods or coccobacilli Oxidase: pos Indole: pos Motile: pos Sucrose: pos Lactose: neg ADH: pos LDC: pos Gelatin: pos Bile esculin: pos Beta hemolysis
Pasteurella spp. Gram negative rods Catalase: pos Oxidase: pos Urea: neg Indole: pos ODC: pos Nitrate: pos
Bartonella spp. Gram negative rods Catalase: neg Oxidase: neg Urea: neg
Brucella spp. Gram negative rods Catalase: pos Oxidase: pos Urea: pos Nitrate: pos
Created by: Ramin11
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