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HTO 140

H & E Staining Trouble shooting

QuestionAnswer
Pale cytoplasmic staining high eosin pH / sections too thin
Dark nuclear staining left too long in hematoxylin
Incomplete deparaffinization incomplete drying not enough time in xylene and water left in tissue
Blue-black precipitate on top of sections unfiltered hematoxylin
nuclear staining not crisp due to incomplete fixation on the processor.
pale nuclear staining not enough time in hematoxylin
Dark basophilic staining around edge of tissue produced from heat artifact, laser techniques
Dark cytoplasmic staining section left in eosin too long
eosin not properly differentiated not enough adequate time in alcohol for paper differentiation
Red or red-brown nuclei Hematoxylin is break down
Created by: Natasha Staley
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