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HTO 140

H&E quiz

QuestionAnswer
Produced from heat artifact; laser techniques Dark basophilic staining around edges of tissue
Left too long in hematoxylin Dark nuclear staining
not enough time in hematoxylin pale nuclear staining
due to incomplete fixation on the processor nuclear staining not crisp
Not enough adequate time in alchol for proper differentiation Eosin not properly differentiated
unfiltered hematoxylin blue-black precipitate on top of sections
incomplete drying, not enough time in xylene and water left in tissue incomplete deparaffinization
section left in eosin too long Dark cytoplasmic staining
hematoxylin is breaking down red or red-brown nuclei
high eosin pH/ sections too thin Pale Cytoplasmic staining
Created by: Natasha Staley
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