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Laboratory Technique

Chemistry, Organic Chemistry, BIochemistry, Biology

TermDefinition
Vacuum Distillation often used when components have very high boiling points and would otherwise be difficult to distill.
Fractional Distillation
Column Chromatography
Boiling Chips provide nucleation sites that give the liquid a place to start forming bubbles to prevent superheating; lower the boiling points of the substances to be distilled. When transitioning from liquid to gas during boiling, the liquid needs nucleation sites, or places to start forming bubbles. This is typically achieved either by scratching the inside of the flask or by introducing boiling chips. They do not alter boiling points of liquids
Centrifugation utilizes a rapidly spinning apparatus to separate particles by density. More dense particles, such as cells, gravitate toward the bottom of the spun tube, while less dense substances remain at the top in a liquid termed the supernatant.
Chromatography
Recrystallization Used to purify a solid product that contains impurities. This process involves the dissolution of the solid in a solvent and subsequent heating. The solid then dissolves and is cooled, causing it to solidify (crystallize) again. As the lattice structures of solids tend to exclude impurities, each subsequent recrystallization results in a progressively purer compound.
nucleation sites places to start forming bubbles. This is typically achieved either by scratching the inside of the flask or by introducing boiling chips.
Vacuum Apparatus removes atmospheric pressure
Distillation to separate compounds according to boiling point. The component with the lower boiling point boils off first, leaving the remaining components in the original flask
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