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Micro Lab #1
Pathogenic Micro Lab Quiz 1 Study Guide
| Question | Answer |
|---|---|
| Describe the position of your hands when carrying the microscope to and from your lab bench? | One hand should be under the base of the microscope to support its weight and one hand should be on the arm for balance. |
| Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eye? | the limit of resolution of the unaided human eye is about .2 mm. for the typical light microscope, the limit is .2 um. |
| What two adjustments can be made to the condenser? what effect do these adjustments have on the image? | the condenser high and diaphragm can be adjusted. illumination of the specimen is increased when the condenser is raised and the diaphragm is opened. |
| Why are condenser adjustments generally preferred over the use of light intensity control? | unlike the voltage control, condenser adjustments will increase illumination without affecting the bulb life. |
| When using the oil immersion lens what four procedures can be implemented to achieve the maximum resolution? | maximum resolution with the oil immersion lens is achieved by using a layer of oil, using a blue filter over the light source, raising the condenser to its highest point, and opening the condenser diaphragm. |
| Why is it advisable to start first with the low power lens when viewing a slide? | Starting with the low power lens, which has a larger working distance, and progressing up to the oil lens is advised. |
| Why is it necessary to use oil in conjunction with the oil immersion lens and not with the other objectives? | |
| What is the relationship between the working distance of an objective lens and its magnification power? | as the power of the objective lens increases the working distance decreases. |
| This objective lens provides the highest magnification. | Oil immersion |
| This objective lens provides the second highest magnification. | high-dry |
| This objective has the lowest magnification. | low power |
| This objective has the shortest working distance. | oil immersion |
| The coarse focus knob should be adjusted only when using this objective lens. | low power |
| This lens collects and focuses light from the lamp onto the specimen on the slide. | condenser |
| This lens, also known as the eyepiece, often comes in pairs. | ocular |
| Diopter adjustments can be made to this lens. | ocular |
| A diaphragm is used to regulate light passing through this lens. | condenser |
| Acetone is the safest solvent for cleaning an objective lens. | False |
| Only lint free, optically safe tissue should be used to wipe off microscope lenses | True |
| The total magnification capability of a light microscope is only limited by the magnifying power of the lens system | False |
| The coarse focus knob can be used to adjust the focus when using any of the objective lenses. | False |
| Once focus is achieved at one magnification, a higher power objective lens can be rotated into position without fear of striking the slide. | True |
| The resolving power of a microscope is a function of: | numerical aperture of the lens and the wavelength of light |
| The coarse and fine focus knobs adjust the distance between: | stage and the objective lens |
| A microscope that maintains focus when the objective magnification is increased is called: | Parfocal |
| The total magnification achieved when using a 100x oil immersion lens with a 10x binocular eyepiece is: | 1000x |
| The most useful adjustment for increasing image contrast in low power magnification is: | closing down the diaphragm |
| Before the oil immersion lens is rotated into place, you should: | center the object of interest in the preceding lens and place a drop of oil on the slide. |
| Resolving Power | Is the lens that separates 2 objects in a microscope field. It's given by the formula d=0.5 /\/ NA. |
| Depth of field | Is the range of distance in front of and behind a focused image within which other objectives will appear clear and sharply defined. |
| Parfocal | Is a lens that stays in focus when magnification / focal length is changed. |
| Steps for preparing a wet mount (first 2) | 1. Use a clean slide and coverslip 2. Insert the pipette into the bottom of the sample bottle to obtain the maximum number of organisms. |
| Steps for preparing a wet mount (last 2) | 3. Remove filamentous algae using forceps. Avoid using too much material. ( Pipette a drop of pond water onto a microscope slide and cover the drop with a coverslip.) |
| what can good smears be used for? | Good smears can prepare for identifying bacterium, morphology and arrangement of cells, and internal structures. |
| what are the goals for making a good smear? | By being successful with your smear allows for certain results in identifying unknown bacteria. |
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