Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Safety
Lab
| Question | Answer |
|---|---|
| 3 types lab hazards? | Biological/infectious, mechanical, chemical |
| 4 types of infectious waste (hazards) per the CDC & EPA? | Microbiologic/cultural material, pathologic material, blood, sharp objects |
| Mechanical hazards include? | Sharp instruments, glass, electronic hazards, latex sensitivity, poor ergonomics |
| Chemical hazards include? | Toxic, corrosive, fire, explosion hazards |
| For chemical safety OSHA requires employers to? | Provide MSDS, label chemicals, maintain hazard communication program, maintain chemical hygiene plan, train employees who use hazardous chemicals, inform employees who don't work w/ chemicals of hazards |
| 3 ways hazardous chemicals enter the body & how to prevent? | Ingestion - no eating/drinking/smoking, absorption - provide gloves, inhalation - provide fume hoods |
| PEL | Permissible exposure limit, amount of chemical exposed to averaged over 8 hrs |
| STEL | Short-term exposure limit, Max amount in 15 min |
| TWA | Time-weighted average, measured concentration calculated to 8 hr exposure |
| Formaldehyde PEL & STEL? | PEL 0.75 ppm, STEL 2.0 ppm |
| Toxic substance hazards include? | Cancer, tumors, defects in offspring, death, irritation/sensitization, diminution of mental alertness |
| Common toxic chemicals? | Formaldehyde, mercury, chromium, silver, hydrocarbons |
| Corrosive substances are those that_ | By direct contact destroy mild steel faster than 6.35mm/yr |
| Common corrosive chemicals? | Acids |
| Corrosive substance hazards include? | Injury to skin, eyes by contact, damage to respiratory, alimentary by inhalation/ingestion, disruption of cell membranes, coagulation of proteins |
| A flammable solvent is a_ | Liquid w/ flash point <60°C & vapor pressure <40 psi at 37.8°C |
| Flash point of toluene? | 4.4°C |
| Flash point of xylene? | 27.2°C |
| Flash point of isopentane? | < -76°C |
| Flash point of benzene? | 0°C |
| Flash point of methanol? | 11.1°C |
| Flash point of ethanol? | 12.8°C |
| Flash point of isopropanol? | 11.7°C |
| 4 criteria EPA uses to classify waste as hazardous | Ignitability, corrosivity, reactivity, extraction procedure toxicity |
| Ignitability hazard if_ | Nonaqueous liquid <24% alcohol w/ flash point <60°C |
| Corrosivity hazard if_ | Aqueous solution w/ pH <=2 or >=12.5 |
| Reactive substances are those that_ | react violently w/ water, are unstable, explosive, contain cyanide or sulfide & generate toxic gas at pH 2 to 12.5 |
| Extraction procedure toxicity tested by__ | Extracting waste @ pH 5, & analyzing for arsenic, barium, cadmium, chromium, lead, mercury, selenium, silver |
| NFPA | National Fire Protection Association |
| NFPA number hazard rating system | 4 - severe 3 - serious 2 - dangerous 1 - minor 0 - none |
| NFPA color hazard rating system | Yellow - reactivity Red – flammability hazard White -special hazard symbol Blue - health |
| Class A fire extinguishers use_ | Water-based foam, multipurpose dry, loaded stream |
| Class B fire extinguishers use__ | CO2, Halon, dry chemical, foam, loaded stream |
| Class C fire extinguishers use__ | Halon, CO2, dry chemical |
| Class D fire extinguishers use__ | Non-reactive dry powder media |
| Class A fires are__ | Wood, paper plastic, textiles fires, extinguish w/ water |
| Class B fires are__ | Flammable liquids, gas fires, block oxygen |
| Class C fires are__ | Electrical fires, extinguish w/ non-conductive media |
| Class D fires are__ | Combustible & reactive metal fires, difficult to extinguish |
| EPA | Environmental Protection Agency, regulatory & enforcing agency for hazardous waste |
| CDC | Center for Disease Control, advisory agency to EPA |
| OSHA | Occupational Safety & Health Administration |
| PPE | Personal protective equipment; gloves, face shield, glasses, coat, apron, breathing protection |
| Sharps containers must be: | Color coded, closable, puncture-resistant, upright, easy access, easy to see when full; regulated by OSHA under blood-borne pathogens |
| MSD's are: | Musculoskeletal disorders/cumulative trauma disorders, e.g. carpal tunnel, trigger finger, tendonitis, thoracic outlet compression syndrome |
| Bloodborne Pathogen Standard | OSHA, 1993, to protect workers who may come into contact w/ potentially infectious material |
| Universal Precautions | Treat all specimens as if highly contagious |
| CJD | Creutzfeldt-Jakob disease, caused by very stable & resistant prions, causes spongiform changes to cortex & subcortical white matter, transmissible from animals to humans, latency period of 20+ yrs, |
| How to dispose of microbiologic or culture material & blood? | Down the drain w/ bleach, if known pathogen-free just water |
| How to dispose of pathological material & sharps hazards? | Incinerate or steam sterilize |
| Prions are resistant to? | Formalin, organic solvents, enzymes, heat, ionizing radiation, freezing, drying, autolysis |
| Prions are susceptible to? | Autoclaving 1 hr @ 121 °C, 1N NaOH 1 hr, conc'd formic acid 1 hr, 5% sodium hypochlorite 2 hr |
| Fix tissue for known or suspected CJD: | Fix brain in formalin approx. 48 hrs, or phenol sat'd formalin 72 hrs, whole brain fixed 10-14 days, tissue in conc'd formic acid 1 hr, tissue in fresh formalin 48 hrs |
| Solutions & equipment used w/ suspected CJD tissue must be neutralized with? | 1N NaOH 1 hr |
| Hazard Communication Standard | OSHA, 1988, "Right to Know Law," workers must be informed of hazards of chemicals used in workplace so they can protect themselves |
| Occupational Exposure to Hazardous Chemicals in the Laboratory Standard | "The laboratory Standard," mandates existence of chemical hygiene plan |
| Chemical hygiene plan includes: | Chemical hazard info, chemical use training, SDS training, chemical inventory, proper labeling of chemicals, SOPs, inform non-employees of lab hazards |
| The Formaldehyde Standard | 1992-1993, sets exposure limits & action levels |
| Fire: | Rapid oxidation in presence of ignition source w/ liberation of heat & light |
| Fire triangle: | fuel, ignition source, oxygen |
| Flash point: | Lowest temperature @ which sufficient vapors prod'd to form ignitable mixture w/ air near surface of liquid/container |
| CESQG | Conditionally exempt small quantity generator |
| SQG | Small quantity generator |
| LQG | Large quantity generator |
| SAA | Satellite waste accumulation area |
| Acids may be disposed of down the drain if__ | They are between pH 3 and pH 11 |
| What does LD50 mean? | LD50 is the calc'd doze of a chemical that is expected to kill 50% of an experimental population |
Created by:
CCF
Popular Histology sets