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Histo Exam 1 - P/F
Properties and Fixatives
Property | Fixative |
---|---|
chromaffin granules | Orth (potassium dichromate) |
connective tissue | Bouin's |
electron microscopy | Glutaraldehyde, Modified Millonig, Osmium tetroxide, Paraformaldehyde, Zamboni |
enzymes | Acetone. Frozen sections – no fixative |
erythrocytes | Preserved by formalin; NBF, Helly Lysed by acetic acid: Bouin, Carnoy, Zenker |
fat | osmium tetraoxide, Bouin's |
Feulgen Reaction for DNA | avoid Bouin's |
Frozen section | Routine stains - fix in formalin |
Glycogen | absolute alcohol, Gendre |
H. pylori | Avoid Glycoxal |
Immunofluorescence | Frozen section without fixation |
Immunoreactivity | Zinc Formalin |
Lymph nodes/nuclear detail | B-5 |
pheochromocytoma | Ortho |
PTAH stain for muscle cross-striation | Zenker |
urate crystals | absolute alcohol |
other special stains | formalin, most commonly |
Special studies requiring unfixed tissue | michel's transport media |
nucleic acids | acetic acid, alcohol, and Carnoy |
stains for DNA or RNA | Avoid picric acid |
Dissolves small calcium deposits | Bouin's, Hollande, Zenker, acetic acid, glycoxal |
Cell culture and tissue culture | RPMI |