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Histo Exam 1 - P/F

Properties and Fixatives

PropertyFixative
chromaffin granules Orth (potassium dichromate)
connective tissue Bouin's
electron microscopy Glutaraldehyde, Modified Millonig, Osmium tetroxide, Paraformaldehyde, Zamboni
enzymes Acetone. Frozen sections – no fixative
erythrocytes Preserved by formalin; NBF, Helly Lysed by acetic acid: Bouin, Carnoy, Zenker
fat osmium tetraoxide, Bouin's
Feulgen Reaction for DNA avoid Bouin's
Frozen section Routine stains - fix in formalin
Glycogen absolute alcohol, Gendre
H. pylori Avoid Glycoxal
Immunofluorescence Frozen section without fixation
Immunoreactivity Zinc Formalin
Lymph nodes/nuclear detail B-5
pheochromocytoma Ortho
PTAH stain for muscle cross-striation Zenker
urate crystals absolute alcohol
other special stains formalin, most commonly
Special studies requiring unfixed tissue michel's transport media
nucleic acids acetic acid, alcohol, and Carnoy
stains for DNA or RNA Avoid picric acid
Dissolves small calcium deposits Bouin's, Hollande, Zenker, acetic acid, glycoxal
Cell culture and tissue culture RPMI
Created by: liviticus