Save
Busy. Please wait.
or

show password
Forgot Password?

Don't have an account?  Sign up 
or

Username is available taken
show password

why


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.


Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
focusNode
Didn't know it?
click below
 
Knew it?
click below
Don't know
Remaining cards (0)
Know
0:00
share
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

Embedding

Lab

QuestionAnswer
Supporting/embedding medium __ Holds cells & intercellular structures in proper relationship
Most popular embedding medium? Paraffin wax
As paraffin melting point increases ___ Paraffin becomes harder, provides better support
As paraffin melting point decreases __ Paraffin becomes softer, provides less support
Harder paraffin easier__ Thin sections
Softer paraffin easier __ Ribboning
Most common paraffin melting point? 55 °C to 58 °C
Sections may be processed at edge, soft in middle because? Sections too thick for good infiltration
Fix inadequately processed blocks b/c thick tissue? Trim unprocessed tissue off, embed, Trim sections down, xylene, tetrahydrofuran, xylene, re-infiltrate, embed
Carbowax embedding media are? Water-soluble waxes, polyethylene glycol+wax, blocks aren't permanent, dry up
Use of carbowax media doesn't require? Dehydration & clearing - waxes infiltrate directly from aq. fixatives, fat preserved
Carbowax won't infiltrate? Tissue w/ lots of fat, CNS infiltration v slow
Biggest problem w/ Carbowax + fix? Sections dissolve & "float out" on bath; To help add to bath : potassium dichromate+gelatin+water, diethylene glycol+formaldehyde +Carbowax+water
Carbowax sections are cut__? Chilled, 3 to 5 μm
Celloidin is? Any nitrocellulose embedding medium, use w/ any fixative
Most commonly used celloidin? Parlodion
Celloidin sections can be cut? Wet; keep wet w/ 80% until stained Dry: infiltrate w/ cedarwood oil first
Celloidin great for? CNS
Celloidin not often used b/c? Processing slow (weeks to months), uses very hazardous & explosive reagents, blocks can't be stored long
Celloidin processing does not require? Heat; less damage to tissue
GMA embedding medium Glycol methacrylate, acrylic resin, tolerates some water
GMA good for? Undecalcified bone, bone marrow, kidney, lymph node biopsies
GMA staining is__ More difficult than paraffin, some stains impossible b/c medium not removed from sections
Epoxy resins require__ Dehydrant & transitional fluid (like clearing agent for paraffin)
Most common epoxy transitional fluid? Propylene oxide
Most common epoxy resins? Araldite, Epon, & Spurr's, may cause dermatitis
Epoxy section thickness? Normal: 60-90 nm, thick: 0.5 μm
Agar & gelatin media used to? Make block w/ friable tissue Maintain specimen orientation during processing (double impregnation) Make frozen blocks (harden w/ formalin)
30% Sucrose use Infiltrate formalin-fixed, unprocessed tissue for frozen blocks
Tissue to be embedded diagonally? Hard tissues, e.g. bone
Tissue to be embedded on-edge? Tissues w/ wall or epithelium e.g. cysts, gallbladder, GI, skin, orient so knife through softest surface first
Tissues to be embedded in cross-section? Tubular structures
Forceps metastasis Fragments of tissue from one block are transferred to another on forceps, can lead to misdiagnosis, clean forceps between blocks
Blocks should be cooled quickly so__? Smaller paraffin crystals form, better support
Too much heat during processing or embedding may? Overfix/overharden tissue, negatively affect stainability
Celloidin processing procedure Dehydrate w/ equal parts 95% & 100%, Treat w/ equal mix 100%+ether, Infiltration w/ celloidin mixed w/ equal parts 100% & ether
Celloidin infiltration procedure Starts at 2% celloidin, gradually up to 12% to 14% celloidin Blocks hardened w/ chloroform
Microchatter Parallel tears in tissue potentially caused by overprocessed/overdehydrated tissue
Sponge artifact Caused by dry sponges being compressed into tissue
Best embedding medium for high-resolution light microscopy? GMA
Temperature of melted paraffin used for embedding? 2°C to 4°C above melting point
Polyethylene glycol can be used as embedding medium for? Lipids
OCT compound Optimal cutting temperature compound; water-soluble embedding medium, freeze quickly w/ liquid nitrogen, frozens
Other terms for embedding? Casting, blocking
Created by: CCF
 

 



Voices

Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!

"Know" box contains:
Time elapsed:
Retries:
restart all cards