Busy. Please wait.

show password
Forgot Password?

Don't have an account?  Sign up 

Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
Didn't know it?
click below
Knew it?
click below
Don't know
Remaining cards (0)
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

H&E Trouble-Shooting


White spots in section after deparaffinization Section not dried enough or not in xylene long enough; place in absolute alcohol then xylene or xylene longer
Nuclei too pale (hematoxylin light) Not in hematoxylin long enough, hematoxylin overoxidized, differentiation too long; restain
Nuclei overstained (hematoxylin dark) Too long in hematoxylin, sections too thick, differentiation too short, Decolorize, restain, check pH, rinse bluing cup, dehydrate quickly
Red, reddish-brown nuclei Hematoxylin old, hematoxylin not ripened, insufficient bluing (can't overblue)
Pale staining w/ eosin Eosin pH >5 (maybe bluing carryover), sections too thin, over-dehydrated
Cytoplasm overstained, differentiation poor Eosin too conc'd (eosin-phloxine), section stained too long, insufficient dehydration, eosin in alcohols
Blue-black precipitate on top of sections Overoxidzed hematoxylin; filter hematoxylin
Microscopic water bubbles in section Incomplete dehydration; alcohol, xylene, recoverslip
Difficulty bringing some ares or tissue in focus w/ light microscope Mounting medium on coverslip; recoverslip
Mounting medium retracted from edge of coverslip Coverglass warped or mounting medium thinned w/ too much xylene; recoverslip, cap mounting medium
Water & slides turn milky following rehydrating alcohols Xylene incompletely removed; change alcohols, dehydrate slides
Slides hazy & milky in last xylene Water in xylene, change alcohol, redehydrate, change xylene
Mounted, stained sections don't show usual transparency & crispness under light microscope Mounting medium too thick; recoverslip
Hazy blue nuclei Too much heat on processor, too hot paraffin, under-fixed; begin dehydration w/ 65% to 70%
Uneven H&E staining, nuclei show poor chromatin detail Water in clearant, water or fixative in infiltrating paraffin, solution levels insufficient; if open processor in high humidity use toluene instead of xylene, if closed processor check for malfunction
Dark basophilic staining of nuclei & cytoplasm, esp. around edges of tissue Laser & electrocautery heat artifact, can't fix
Brown stippling resembling pigment & section shows glossy black nuclei Section air-dried before coverslipping; remove coverslip, set in water several min, dehydrate, clear, remount
"Cornflaking" artifact caused by? Section drying before it is coverslipped
Poor contrast between nuclei & cytoplasm Understained nuclei, overstained cytoplasm; run control, check/change solutions
Less than three shades of eosin Increase time in low dehydrating alcohols, best eosin differentiation occurs in 70%
Created by: CCF



Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!

"Know" box contains:
Time elapsed:
restart all cards