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D Bac Exam 2

Enterobacteriaceae gram postive rods, non spore forming, grow on peptone media, grow on macconkey agar, usually faculatative anaerobes, ferment glucose with gas production, catalase +, oxidase -, reduce nitrate.
Friedlander's Bacillus ("brick red" sputum) K. pneumoniae
endotoxin shock (symptoms) fever, leucopenia, capilary hemorrhage, hypotension, circulatory collapse
limulus lysate assay reagent prepared from the ameboctytes of the horseshoe crab (Limulus polyphemus). Tests for endotoxin shock
lipid A toxic portion of lipopolysaccharide of gram negative cell wall that produces endotoxin
Flagellar antigen H antigen
Capsular antigen K antigen
Somatic antigen O antigen
Ewig developed the concept of tribes with Enterobacteriaceae
tribe I Eschericheae
tribe II Edwardsielleae
tribe III Salmonelleae
tribe IV Citrobactereae
tribe V Klebsielleae
tribe VI Proteeae
tribe VII Yersinieae
Methyl Red Test tests for mixed acid fermentation (red below pH 4.4 and yellow above pH 6.2)
Voges Proskauer Test tests for butanediol fermentation
Embden-Meyerhof pathway series of glycolytic cleavages that result in the production of pyruvic acid from glucose
ONPG-galactose test tests for beta galactosidase and beta galactoside permease, (+ = yellow, - = colorless)
enzyme needed for fermentation of lactose beta galactoside permease and beta galactosidase
Kligler Iron Agar (KIA) contains lactose and glucose (10:1), ferrous sulfate, and phenol red
KIA: alkaline slant (red)/ alkaline butt(red) no carbohydrate fermentation
KIA: alkaline slant (red)/ acid butt (yellow) glucose fermented but lactose not (ex. Shigella)
KIA: alkaline slant (red)/ acid butt (black) glucose fermented but lactose not, production of hydrogen sulfide (Salmonella, Citrobacter, and Proteus)
KIA: acid slant (yellow)/ acid butt (yellow) glucose and lactose fermented (ex. E. coli, Klebsiella, and Enterobactor)
MacConkey Agar differential media for selection and recovery of Enterobacteriaceae
MacConkey Agar: red colonies strong lactose fermenters (ex. Escherichia, Klebsiella, Enterobactor)
MacConkey Agar: pink colonies weak lactose fermenters (ex. Citrobactor, Providencia, Serratia, and Hafnia)
MacConkey Agar: colorless colonies Non lactose fermenters (ex. Proteus, Edwardsiella, Salmonella, Shigella)
Eosin Methylene Blue Agar differentiates between lactose and non lactose fermenters (similar to MacConkey Agar in the detection and differentiation of Enterobacteriaceae)
Eosin Methylene Blue Agar: green black colonies with metallic sheen Strong lactose fermenters (E. coli)
Eosin Methylene Blue Agar: purple colonies weak lactose fermenters
eosin methylene Blue Agar: purple to black colonies sucrose fermenters and non lactose fermenters (ex. Yersinia enterocolitica)
Hektoen Agar highly selective for recovery for Enterocacteriaceae (green media). Good for enhanced recovery of Salmonella and Shigella from material with heavy numbers of normal flora.
Hektoen Agar: bright orange, pink rapid lactose fermenters (E. coli)
Hektoen Agar: blue green colonies with black centers Salmonella
Hektoen Agar: more green colonies Shigella
Xylose lysine deoxycholate agar highly selective media for isolation of enterics, less inhibitory than HE agar (low conc. of bile salts), designed to detect shigellae in feces after enrichment in gram-negative broth.
Selenite Broth used for recovery of Salmonella and Shigella from feces or sewage. maintain predominant E. coli in lag phase
ingredient in Selenite broth that inhibits E. coli sodium selenite
indole produced by the breakdown of trptophan, also produce is pyruvate and ammonia
tryptophanase enzyme that breaksdown tryptophan
Kovac's reagent p-dimethylaminobenxaldehyde, used in the detection of indole (red = +)
Citrate utiliatization tests ability of strain to utilize citrate as a sole carbon and energy source
Citrate utilization: positive result blue color
E. coli indole +, methyl red +, VP -, Citrate -
Enterobacter aerogenes indole -, methyl red -, VP +, citrate +
Created by: rburt