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ch 47 micro
Parasite Lab Methods
Question | Answer |
---|---|
Parasites of humans are classified into how many divisions? | 6 |
Name the 6 divisions of human parasites | protozoa, nematoda, platyhelminthes, pentastomids, acanthocephala, arthropoda |
name the 6 types of protozoa | amebae, flagellates, ciliates, sporozoans, coccidia, microsporidia |
nematodes are also known as | roundworms |
playthelminthes are also known as | flatworms |
pentastomids are also known as | tongue worms |
acanthocephala are also known as | thorn or hook worms |
arthropoda consist of | insects, spiders, mites or ticks |
How many routes of transmission are there in human parasites? | 4 |
Name the routes of transmission of parasites in humans | 1. venereal, 2. ingestion of contaminated food or water, 3. skin penetration of infective larvae, 4. bites of various arthropods |
Name 3 arthropods of human parasiticsm | plasmodium, trypanosoma, leishmania |
Name 3 human parasites of skin penetration of infective larvae | Strongyloides stercoralis, hookworm, schistosomes |
Name 2 parasites of contaminated food and water | Giardia lamblia, Cryptosporidium spp. |
name of a parasite transmitted venereally | Trichomonas vaginalis |
transmission of cryptosporidium spp | ingestion of contaminated food and water |
transmission of Giardia lamblia | ingestion of contaminated food and water |
transmission of hookworm | skin penetration of infective larvae |
transmission of leishmania | arthropod bite |
transmission of schistosomes | skin penetration of infective larvae |
transmission of plasmodium | bite of arthropod |
transmission of strongyloides stercoralis | skin penetration of infective larvae |
transmission of trichomonas vaginalis | venereal |
transmission of trypanosoma | arthropod bite |
protozoa causes vaginitis | Trichomonas vaginalis |
Stronglyloides stercoralis and hookworm are | intestinal worms |
Schistosomes is a | blood fluke |
plasmodium is the vector of | malaria |
vector is the fly, causes sleeping sickness | Trypanosoma |
vector is the sand fly | Leishmania |
Mulitplication and propagation stage, the obligate stage in the life cycle | spore |
Motile, dividing, metabolizing, very active stage | Trophozoite |
Thick walled stageof coccidian parasites. Shed into the feces of people infected. sexual reproductive stage can be sporulated. | Oocyst |
(Not egg or spore) hibernates, parasitic stage responsible for disease, transmission, a resting and/or dormant resistant stage. | Cyst |
O and P stand for | ova and parasite |
Purpose of PVA | fixes parasite and keeps it alive |
If a sample cannot be transported to the lab immediately it should be | preserved |
Maintains parasite morphologic characteristics | preservatives |
in specimen collection and transport , situations that are considered immediate include: | 1. CNS specimens looking for free-living amebae 2. blood films for potential malaria |
2 vial system commonly used for fecal samples. What are the vials for? | 1. 5-10% formalin for immunoassay and concentration for microscopy, 2. PVA for stained smears |
the 3rd part of the O&P exam is the permanent stained smear; designed to | ID the intestinal protozoa |
Matter that settles to the bottom of a liquid, dregs | Sediment |
coccidian oocysts are identified by | modified acid-fast stain |
purpose of the direct smear | check organism motility |
purpose of concentration | concentrates - protozoan cysts, coccidian oocysts, microsporidian spores, helminth eggs and larvae |
purpose of trichrome stain | provides contrast between background debris and parasite |
formalin is a | preservative |
formalin is called a | fixative |
Purpose of Formalin-ether | Fixes- eggs.larvae oocysts and spores |
Purpose of Trichrome stain | Distinguishes cysts and trophozoites |
Purpose of modified iron hematoxylin stain | Demonstrates cysts and trophozoites |
Purpose of Modified acid-fast stain | Highlights Coccidia |
Formalin is called an xxx-xxxxxxx fixative | all-purpose |
PVA is xxx not a fixative | adhesive |
PVA helps glue the stool material onto the | slide |
Formalin fixes what 4 groups? | eggs, larvae, oocysts and spores |
modified trichrome stains are to identify | Microsporidia spores |
Down side of a wet mount | Can disintegrate immediately |
Type of sample for suspected parasites of the intestinal tract | stool |
3 methods of O and P exam | 1. direct wet mount, 2. concentration, 3. permanent stain |
test for intestinal specimens that goes to the sigmoid colon; bottom part of a colonoscopy. | Sigmoidoscopy |
Test for intestinal specimens, a tube that pulls a vacuum, goes thru the nose down to the duodenum. | Duodenal drainage |
A fecal specimen can be preserved in 10% formalin. What 3 tests can be run? | 1. modified trichrome stain, 2. Elisa, 3. wet mount |
what does the modified trichrome stain test for? | Microsporidia |
What does ELISA test for? | Giardia and Cyclosporidium |
What does the wet mount test for? | Helminths and protozoa |
Fecal specimens can be affixed with PVA. What test is then used on the sample? | Trichrome stain |
What parasite is the trichrome stain used on? | Protozoa |
Fecal specimens can be processed with Formalin-Ethyl acetate sedimentation. What 4 tests are run off this? | 1. Safranin stain, 2. Acid fast stain, 3. Direct mound epifluorescence, 4. wet mount |
what is the safranin stain used to test for? | Cyclospora |
What is the acid-test stain used to test for/ | Cyrptosporidium, Cycloisospora, Cyclospora |
What is the direct mount epifluorescence used to test for? | Cyclospora and Isospora |
following sedimentation what is the wet mount used to test for? | Helminths and protozoa |